Interaction of Bovine Serum Albumin with Cationic Imidazolium Surfactants Containing a Methoxyphenyl Fragment

Kuznetsova, Darya A.; Kuznetsov, Denis M.; Zakharov, V. M.; Zakharova, L. Ya.

doi:10.1134/s1070363222070143

Cited by 5 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the calculated values of K q are at least 20 times higher than the maximum dynamic constant of bimolecular quenching in an aqueous medium (2 × 10 10 L/mol s), which indicates a static mechanism of fluorescence quenching. Most likely, the formation of IA-16-HSA complexes is associated with a mixed quenching mechanism, including both the molecular collision and the formation of a non-covalently bound complex due to the adsorption of surfactant molecules on the hydrophobic domains of the protein [ 34 , 45 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

“…Determination of binding constants of components K a , the number of binding sites n, and thermodynamic characteristics of surfactant–HSA systems was performed on the basis of the primary fluorescent data by Equations (3)–(5) [ 34 ]:

ΔG 0 = ΔH 0 − TΔS 0, where Δ H 0 is the variation of standard enthalpy; Δ S 0 is the variation of standard entropy, R is a universal gas constant (8.314 J·mol −1 ·K −1 ); T is absolute temperature; Δ G 0 is the change of standard Gibbs free energy.…”

Section: Methodsmentioning

confidence: 99%

“…Essentially, the self-aggregation study of mixed surfactant–PE compositions is necessary for a comprehensive description of their solution behavior and functional activity. The knowledge gained can provide a better understanding of optimizing the composition and properties of nanocontainers based on surfactants and PEs; The systems based on surfactant and natural PE are of great importance, e.g., the interaction of cationic surfactants with globular proteins-albumins-is widely studied due to the bioapplication perspective [ 33 , 34 , 35 ]. Albumins are blood transport proteins and have unique properties (commercial availability, water solubility, biodegradability, biogenicity, and ability to accumulate in tumor tissues), which makes them promising candidates for the development of protein-based nanocontainers [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

“…The systems based on surfactant and natural PE are of great importance, e.g., the interaction of cationic surfactants with globular proteins-albumins-is widely studied due to the bioapplication perspective [ 33 , 34 , 35 ]. Albumins are blood transport proteins and have unique properties (commercial availability, water solubility, biodegradability, biogenicity, and ability to accumulate in tumor tissues), which makes them promising candidates for the development of protein-based nanocontainers [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role of Polyanions and Surfactant Head Group in the Formation of Polymer–Colloid Nanocontainers

et al. 2023

Self Cite

View full text Add to dashboard Cite

Objectives. This study was aimed at the investigation of the supramolecular systems based on cationic surfactants bearing cyclic head groups (imidazolium and pyrrolidinium) and polyanions (polyacrylic acid (PAA) and human serum albumin (HSA)), and factors governing their structural behavior to create functional nanosystems with controlled properties. Research hypothesis. Mixed PE–surfactant complexes based on oppositely charged species are characterized by multifactor behavior strongly affected by the nature of both components. It was expected that the transition from a single surfactant solution to an admixture with PE might provide synergetic effects on structural characteristics and functional activity. To test this assumption, the concentration thresholds of aggregation, dimensional and charge characteristics, and solubilization capacity of amphiphiles in the presence of PEs have been determined by tensiometry, fluorescence and UV-visible spectroscopy, and dynamic and electrophoretic light scattering. Results. The formation of mixed surfactant–PAA aggregates with a hydrodynamic diameter of 100–180 nm has been shown. Polyanion additives led to a decrease in the critical micelle concentration of surfactants by two orders of magnitude (from 1 mM to 0.01 mM). A gradual increase in the zeta potential of HAS–surfactant systems from negative to positive value indicates that the electrostatic mechanism contributes to the binding of components. Additionally, 3D and conventional fluorescence spectroscopy showed that imidazolium surfactant had little effect on HSA conformation, and component binding occurs due to hydrogen bonding and Van der Waals interactions through the tryptophan amino acid residue of the protein. Surfactant–polyanion nanostructures improve the solubility of lipophilic medicines such as Warfarin, Amphotericin B, and Meloxicam. Perspectives. Surfactant–PE composition demonstrated beneficial solubilization activity and can be recommended for the construction of nanocontainers for hydrophobic drugs, with their efficacy tuned by the variation in surfactant head group and the nature of polyanions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Role of Polyanions and Surfactant Head Group in the Formation of Polymer–Colloid Nanocontainers

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…The homologous series of MPI-n (n = 10, 12, 14, 16) was obtained via the reaction of 1-(4-methoxyphenyl)imidazole with the corresponding alkyl bromide [47,48]. Briefly: the mixture of 1-(4-methoxyphenyl)-1H-imidazole (1 mol) and corresponding alkyl bromide (1.1 mol) was stirred in dry acetonitrile under heating.…”

Section: Reagents and Probesmentioning

confidence: 99%

Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants

et al. 2022

Self Cite

View full text Add to dashboard Cite

Interaction between cationic surfactants and nucleic acids attracts much attention due to the possibility of using such systems for gene delivery. Herein, the lipoplexes based on cationic surfactants with imidazolium head group bearing methoxyphenyl fragment (MPI-n, n = 10, 12, 14, 16) and nucleic acids (oligonucleotide and plasmid DNA) were explored. The complex formation was confirmed by dynamic/electrophoretic light scattering, transmission electron microscopy, fluorescence spectroscopy, circular dichroism, and gel electrophoresis. The nanosized lipoplex formation (of about 100–200 nm), contributed by electrostatic, hydrophobic interactions, and intercalation mechanism, has been shown. Significant effects of the hydrocarbon tail length of surfactant and the type of nucleic acid on their interaction was revealed. The cytotoxic effect and transfection ability of lipoplexes studied were determined using M-HeLa, A549 cancer cell lines, and normal Chang liver cells. A selective reduced cytotoxic effect of the complexes on M-HeLa cancer cells was established, as well as a high ability of the systems to be transfected into cancer cells. MPI-n/DNA complexes showed a pronounced transfection activity equal to the commercial preparation Lipofectamine 3000. Thus, it has been shown that MPI-n surfactants are effective agents for nucleic acid condensation and can be considered as potential non-viral vectors for gene delivery.

show abstract

Assessment of the assembly behaviour and physicochemical parameters for the tetradecyltrimethylammonium bromide and promazine hydrochloride mixture: Impact of monohydroxy organic compounds

Alam,

Anis-Ul-Haque,

Khan

et al. 2024

Colloid Polym Sci

View full text Add to dashboard Cite

Interaction of Bovine Serum Albumin with Cationic Imidazolium Surfactants Containing a Methoxyphenyl Fragment

Cited by 5 publications

References 42 publications

Role of Polyanions and Surfactant Head Group in the Formation of Polymer–Colloid Nanocontainers

Role of Polyanions and Surfactant Head Group in the Formation of Polymer–Colloid Nanocontainers

Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants

Assessment of the assembly behaviour and physicochemical parameters for the tetradecyltrimethylammonium bromide and promazine hydrochloride mixture: Impact of monohydroxy organic compounds

Contact Info

Product

Resources

About