Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles

Abstract

Nanoparticle size and plasma binding profile contribute to a particle’s longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.

Colloidal stability of nano-sized particles in the peritoneal fluid: Towards optimizing drug delivery systems for intraperitoneal therapy

“…the intraperitoneal fluid in the case of IP delivery. Although several studies have addressed the colloidal stability of nanoparticles in biofluids like blood, plasma and serum [36,37], the physicochemical behavior of delivery vehicles in terms of aggregation and release of cargo in peritoneal fluids has not been investigated yet.…”

Enhanced Radiation Therapy of Gold Nanoparticles in Liver Cancer

“…They are related to many features of the particles themselves, including size and particle surface charge valence. Blood protein coated nanoparticles played an important role in toxicity [56,57]. The characteristics of these proteins, which form a parcel layer, may affect the biological response of nanoparticles, including the impact on the uptake and accumulation in the organs [49].…”

A Review Article on Muscular Dystrophy and Its Management Through Ayurveda

“…According to Dobrovolskaia et al the size range of AuNPs matches with the proteins or even small viruses, hence immune system might also react strongly to it's presence in the body resulting in induced immunotoxicity. [48] RES is part of the immune system with complex components which identify, capture, and filter foreign antigens and particulates. Any potential toxicity arising from AuNPs can be reduced by it's uptake via the RES as well as increase in the circulation time.…”