Interaction of differently functionalized fluorescent silica nanoparticles with neural stem- and tissue-type cells

Izak‐Nau, Emilia; Kenesei, Kata; Kumarasamy, Murali; Voetz, Matthias; Eiden, Stefanie; Puntes, Víctor; Duschl, Albert; Madarász, Emı́lia

doi:10.3109/17435390.2013.864427

Cited by 44 publications

(46 citation statements)

References 52 publications

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“…Despite the wide range of research about nanoparticles (NP) and nanotechnology there are few studies on the effects of these nanomaterials on health and especially on environmental impacts. Silica nanoparticles (SiNP) are extensively used in manufactured products, for example in additives to drugs, in the chemical industry, in printer toners, in cosmetics and in food (Gordon et al, 2009;Van Hoecke et al, 2011;Fede et al, 2012;Izak-Nau et al, 2014;Pisani et al, 2015). Nanoparticle materials that are between 1 and 100 nanometres are usually more toxic than bulk materials of larger size (Sager et al, 2008;Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Toxicity assessment of silica nanoparticles on Allium cepa

Silva¹,

Monteiro²

2024

Ecotoxicol. Environ. Contam.

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Nanotechnology is rapidly expanding, and several new nanoparticle products with improved performance have been developed. The environmental fate and toxicity of nanoparticles (NP) is poorly understood and should be studied to enable the safe use of this technology. This study aims to investigate genotoxic and phytotoxic impacts of silica nanoparticles (SiNP) using root tip cells of Allium cepa as an indicator system. A. cepa root tip cells were exposed to different concentrations (1.82 to 0.54 g L-1 ; dilution factor of 1.5x) of three engineered dispersions of SiNP (TM40-22 nm; HS30-12 nm; SM30-7 nm). The following endpoints were measured: mitotic index, different types of chromosomal aberrations, germination index and root length. A decrease in the mitotic index was observed with increasing NP concentration, and significant decreases in the MI for all tested NP were found. For all NP cytological effects including chromosomal aberrations were observed in treated cells. Phytotoxic effects were also observed as germination rate and root growth were significantly reduced (p<0.05). Plants are an essential component of ecosystems and these findings suggest that they should be included when evaluating the toxicological impact of NP on the environment.

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Section: Introductionmentioning

confidence: 99%

Toxicity assessment of silica nanoparticles on Allium cepa

Silva¹,

Monteiro²

2024

Ecotoxicol. Environ. Contam.

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“…While they are considered to be among the most biocompatible NPs (Huang et al, 2005;Izak-Nau et al, 2014;Kim et al, 2006), extensive knowledge of the cellular responses elicited by their interaction is still incomplete. This is of particular relevance for the nervous system (NS): nanoneuromedicine is rapidly gaining momentum (Gendelman et al, 2014),and silica NPs are good candidates for applications in this field, ranging from drug delivery (Rosenholmet al, 2011) to cell tracking and subcellular imaging (Montalti et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis

Gilardino

Catalano

Alberto

et al. 2015

The International Journal of Biochemistry & Cell Biology

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SiO 2 nanoparticles (NPs), in addition to their widespread utilization in consumer goods, are also being engineered for clinical use. They are considered to exert low toxicity both in vivo and in vitro, but the mechanisms involved in the cellular responses activated by these nanoobjects, even at non toxic doses, have not been characterized in detail.This is of particular relevance for their interaction with the nervous system: silica NPs are good candidates for nanoneuromedicine applications. Here, by using the GT1-7 neuronal cell line, derived from gonadotropin hormone releasing hormone (GnRH) neurons, we describe the mechanisms involved in the perturbation of calcium signaling, a key controller of neuronal function. At the non toxic dose of 20 µg mL -1 , 50nm SiO 2 NPs induce long lasting but reversible calcium signals, that in most cases show a complex oscillatory behavior. Using fluorescent NPs, we show that these signals do not depend on NPs internalization, are totally ascribable to calcium influx and are dependent in a complex way from size and surface charge. We provide evidence of the involvement of voltage-dependent and transient receptor potential-vanilloid 4 (TRPV4) TRPV4 channels.

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“…Quantum dots Apoptosis in mouse blastocysts, inhibited cell proliferation, early-stage blastocyst death in mouse [60] Apoptosis in mouse myoblast cells line [62] Impaired hematopoiesis in an invertebrate model organism [63] Fluorescent silica nanoparticles Abnormal morphology and altered gene expression of human neural stem cells [67] Cell death at high particle doses in neural stem cells [68] Enhanced proliferation of human adipose tissuederived stem cells through ERK1/2 activation [69] Metabolic stress in hMSCs through EGR1, CCND, and E2F1 genes [70] MSC: mesenchymal stem cells; hMSC: human mesenchymal stem cells…”

Section: Superparamagnetic Iron Oxide Nanoparticlesmentioning

confidence: 99%

“…These results may indicate the potential toxicity of accumulated NPs for long-term usage or continuous exposure. Silica NPs with different [-NH2, -SH and poly(vinylpyrrolidone) (PVP)] surface modifications cause cell death at high particle doses, except for PVP-coated SiO2 NPs [68]. Among the tested neural tissue-type cells, neural stem cells and astrocytes internalise plain SiO2, SiO2-NH2 and SiO2-SH NPs, while neurons do not take up any NPs at all.…”

Section: Fluorescent Silica Nanoparticlesmentioning

confidence: 99%