Interaction of DNA Minor Groove Binder Hoechst 33258 with Bovine Serum Albumin

Ojha, Himanshu; Murari, Bhaskar Mohan; Anand, Sneh; Hassan, Md. Imtaiyaz; Ahmad, Faizan; Chaudhury, N. K.

doi:10.1248/cpb.57.481

Cited by 47 publications

(23 citation statements)

References 43 publications

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“…Although high glass transition temperatures would keep the vaccine more stable during storage, replacing PVP by BSA enhanced vaccine virus stability (Table 1). Probably other stabilizing mechanisms such as hydrophobic interaction of BSA [22][23][24] with the enveloped ND vaccine were responsible for the vaccine storage stability, while PVP can only engage in hydrophilic interactions [25].…”

Section: Discussionmentioning

confidence: 99%

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Huyge

Reeth

Beer

et al. 2012

European Journal of Pharmaceutics and Biopharmaceutics

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Dry powders containing a live-attenuated Newcastle disease vaccine (LZ58 strain) and intended for mass vaccination of poultry were prepared by spray drying using mannitol in combination with trehalose or inositol, polyvinylpyrrolidone (PVP) and/or bovine serum albumin (BSA) as stabilizers. These powders were evaluated for vaccine stabilizing capacity during production and storage (at 6ºC and 25ºC), moisture content, hygroscopicity and dry powder dispersibility. A mixture design, varying the ratio of mannitol, inositol and BSA, was used to select the stabilizer combination which resulted in the desired powder properties (i.e. good vaccine stability during production and storage, low moisture content and hygroscopicity and good dry-dispersibility). Inositol-containing powders had the same vaccine stabilizing capacity as trehalose powders, but were less hygroscopic. Incorporation of BSA enhanced the vaccine stability in the powders compared to PVP-containing formulations. However, increasing the BSA concentration increased the hygroscopicity and reduced the dry dispersibility of the powder. No valid mathematical model could be calculated for vaccine stability during production or storage, but the individual experiments indicated that a formulation combining mannitol, inositol and BSA in a ratio of 73.3:13.3:13.3 (wt/wt) resulted in the lowest vaccine titre loss during production (1.6-2.0 log 10 50% egg infectious dose (EID 50 ) and storage at 6ºC (max. 0.8 log 10 EID 50 after 6 months) in combination with a low moisture content (1.1-1.4%), low hygroscopicity (1.9-2.1% water uptake at 60% relative humidity) and good dry-dispersibility properties.

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Section: Discussionmentioning

confidence: 99%

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Huyge

Reeth

Beer

et al. 2012

European Journal of Pharmaceutics and Biopharmaceutics

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“…Thus, letting tumor cells to apoptosis is a requirement for an anticancer agent. It is generally accepted that cytotoxic drugs eliminate malignant cells by inducing apoptosis 32,33 . The activation of caspases, a family of important signaling molecules with various tasks depending on subtype and organ, is a marker for apoptosis 34 .…”

Section: Analysis Of Caspase-3 Activationmentioning

confidence: 99%

“…Dacarbazine, an imidazole compound, inhibits DNA, RNA and protein synthesis by alkylation mechanism 31 . Bisbenzimidazole compounds such as Hoechst 33342 and Hoechst 33258 have anticancer ability to inhibit DNA topoisomerase I and many other cellular processes 32 . Natural bis(benzoxazole) product UK-11 shows cytotoxic action on cancer cells, selectively 33 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and anticancer activity evaluation of N-[4-(2-methylthiazol-4-yl)phenyl]acetamide derivatives containing (benz)azole moiety

Yurttaş¹,

Özkay²,

Akalın-Çiftçi³

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…HSA has multiple binding sites for lower and higher fatty acids, and there are dedicated binding sites for aromatic compounds. Specific binding sites named Sudlow I and II are characterized by their binding of aromatic compounds . The amino acid sequence of HSA contains 17 disulfide bridges, one free thiol (Cys34) and a single tryptophan (Trp214), which may be vital when HSA binds with ligands along the chain …”

Section: Introductionmentioning

confidence: 99%

“…In the circulation, the binding process between a particular molecule and serum albumin may impact the structure of HSA. In other words, pathological factors can significantly alter the binding of ligands, and one such pathological condition is excess glucose . As the level of glucose increases, HSA is non‐enzymatically glycated to reduce sugar from blood.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic andin silicostudy of binding mechanism of cynidine‐3‐O‐glucoside with human serum albumin and glycated human serum albumin

Gao

Jia

et al. 2016

Luminescence

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The drug-serum albumin interaction plays a dominant role in drug efficacy and disposition. The glycation of serum albumin that occurs during diabetes may affect its drug-binding properties in vivo. In order to evaluate the interactivity characteristics of cyanidin-3-O-glucoside (C3G) with human serum albumin (HSA) and glycated human serum albumin (gHSA), this study was undertaken using multiple spectroscopic techniques and molecular modeling analysis. Time-resolved fluorescence and the thermodynamic parameters indicated that the quenching mechanism was static quenching, and hydrogen bonding and Van der Waals force were the main forces. The protein fluorescence could be quenched by C3G, whereas the polarity of the fluorophore was not obviously changed. C3G significantly altered the secondary structure of the proteins. Furthermore, the interaction force that existed in the HSA-C3G system was greater than that in the gHSA-C3G system. Fluorescence excitation emission matrix spectra, red edge excitation shift, Fourier transform infrared spectroscopy and circular dichroism spectra provided further evidence that glycation could inhibit the binding between C3G and proteins. In addition, molecular modeling analysis supported the experimental results. The results provided more details for the application of C3G in the treatment of diabetes.

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Interaction of DNA Minor Groove Binder Hoechst 33258 with Bovine Serum Albumin

Cited by 47 publications

References 43 publications

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Synthesis and anticancer activity evaluation of N-[4-(2-methylthiazol-4-yl)phenyl]acetamide derivatives containing (benz)azole moiety

Spectroscopic andin silicostudy of binding mechanism of cynidine‐3‐O‐glucoside with human serum albumin and glycated human serum albumin

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