Interaction of doxorubicin and its derivatives with DNA: Elucidation by resonance Raman and surface-enhanced resonance Raman spectroscopy

Yan, Qing; Priebe, Waldemar; Chaires, Jonathan B.; Czernuszewicz, Roman S.

doi:10.1002/(sici)1520-6343(1997)3:4<307::aid-bspy6>3.0.co;2-0

Cited by 42 publications

(35 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most intense band within the simulated Raman spectrum is found at approximately 1396 cm −1 , which may be attributed to the phenyl ring vibration, and it is instead observed at approximately 1412 and 1419 cm −1 in the experimental spectra. Raman spectra are similar to the findings of the published works (Yan et al, 1998; Strekal et al, 2001).…”

Section: Resultssupporting

confidence: 90%

FT‐IR, Raman, RRS measurements and DFT calculation for doxorubicin

Das¹,

Nicastri²,

Coluccio³

et al. 2010

Microscopy Res & Technique

110

View full text Add to dashboard Cite

Doxorubicin (DOXO) is a powerful anthracycline antibiotic used to treat many human neoplasms, including acute leukemias, lymphomas, stomach, breast and ovarian cancer, and bone tumors, yet causing cardiotoxicity at the same time. For this reason, there is a great interest in medical field to gain deep insight and knowledge of this molecule. Raman, Fourier Transform Infrared (FT-IR) absorption spectroscopy, and Resonance Raman scattering were performed for the vibrational characterization of DOXO molecule. Density function theorem (DFT) modeling of Raman and FT-IR spectra were used for the assignment of the vibrational frequencies. The optimized molecular structured was obtained, first, on the basis of potential energy distribution. The simulation for vibrational bands is based on the calculations for internal force constants and potential energy distribution matrices. The calculated DOXO vibrational bands show qualitative agreement with the experimental observations (FT-IR absorption and Raman scattering).

show abstract

Section: Resultssupporting

confidence: 90%

FT‐IR, Raman, RRS measurements and DFT calculation for doxorubicin

Das¹,

Nicastri²,

Coluccio³

et al. 2010

Microscopy Res & Technique

110

View full text Add to dashboard Cite

show abstract

“…Therefore, this shift of the band of phenanthridinium ring could be used as a criterion of the EB chromophore intercalation between the base pairs of DNA. A similar effect was observed in Raman spectra of some aromatic components intercalated into DNA [16].…”

Section: Introductionsupporting

confidence: 76%

Investigation of complexation of ethidium bromide with DNA by the method of Raman spectroscopy

et al. 2009

View full text Add to dashboard Cite

The investigation of complexation features of ethidium bromide (EB) with calf thymus DNA at the high and low ratios of biopolymer/ligand molar concentrations (P/D) was carried out using Raman spectroscopy and VIS-spectrophotometry. It was shown that EB binds to DNA with formation of two types of complexes: intercalation and exterior binding. The analysis of Raman spectra revealed that the amino groups of EB form the hydrogen bonds with acceptor atom groups of DNA in both types of complexes. A low extent of filling DNA structure by the ligand (P/D = 20) does not change the DNA B-form, while a high extent (P/D = 3) results in the conformation B-A transitio

show abstract

“…[26], [27], [28] To examine whether such interaction occurs with modified TLS11a-GC and TD05-GC aptamers, fluorescence was acquired for Dox in the absence and in the presence of one of the aptamers (Fig. 2c).…”

Section: Resultsmentioning

confidence: 99%

Targeted Delivery of Chemotherapy Agents Using a Liver Cancer-Specific Aptamer

et al. 2012

View full text Add to dashboard Cite

BackgroundUsing antibody/aptamer-drug conjugates can be a promising method for decreasing toxicity, while increasing the efficiency of chemotherapy.Methodology/Principal FindingsIn this study, the antitumor agent Doxorubicin (Dox) was incorporated into the modified DNA aptamer TLS11a-GC, which specifically targets LH86, a human hepatocellular carcinoma cell line. Cell viability tests demonstrated that the TLS11a-GC-Dox conjugates exhibited both potency and target specificity. Importantly, intercalating Dox into the modified aptamer inhibited nonspecific uptake of membrane-permeable Dox to the non-target cell line. Since the conjugates are selective for cells that express higher amounts of target proteins, both criteria noted above are met, making TLS11a-GC-Dox conjugates potential candidates for targeted delivery to liver cancer cells.Conclusions/SignificanceConsidering the large number of available aptamers that have specific targets for a wide variety of cancer cells, this novel aptamer-drug intercalation method will have promising implications for chemotherapeutics in general.

show abstract

Interaction of doxorubicin and its derivatives with DNA: Elucidation by resonance Raman and surface-enhanced resonance Raman spectroscopy

Cited by 42 publications

References 35 publications

FT‐IR, Raman, RRS measurements and DFT calculation for doxorubicin

FT‐IR, Raman, RRS measurements and DFT calculation for doxorubicin

Investigation of complexation of ethidium bromide with DNA by the method of Raman spectroscopy

Targeted Delivery of Chemotherapy Agents Using a Liver Cancer-Specific Aptamer

Contact Info

Product

Resources

About