Interaction of fibrinogen with solid surfaces of varying charge and hydrophobic—hydrophilic balance

Schmitt, A.; Varoqui, R.; Uniyal, S; Brash, John L.; Pusineri, Christian

doi:10.1016/0021-9797(83)90113-3

Cited by 125 publications

(55 citation statements)

References 27 publications

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“…11,[54][55][56] We and others previously suggested that most fibrinogen molecules are oriented vertically on the surface when immobilized at high density and horizontally at low density. [57][58][59][60] Thus, on high-density fibrinogen, the ␣IIb␤3 recognition sites on the ␥-chain C-termini are likely to extend out from the surface, whereas on low-density fibrinogen the horizontal orientation is likely to reduce access to the ␥-chain C-termini and/or induce conformational changes by multiple contacts between fibrinogen molecules and the surface. This may expose regions that are cryptic in solution [61][62][63] and make them available for the interaction with the integrin, maybe contributing to the unique outside-in signaling observed on low-density fibrinogen.…”

Section: Discussionmentioning

confidence: 99%

Ligand density dramatically affects integrin αIIbβ3-mediated platelet signaling and spreading

Jiroušková

Jaiswal

Coller

2007

Blood

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The impact of ligand density on integrinmediated cell adhesion and outside-in signaling is not well understood. Using total internal reflection fluorescent microscopy, conformation-specific antibodies, and Ca 2؉ flux measurements, we found that the surface density of fibrinogen affects ␣IIb␤3-mediated platelet signaling, adhesion, and spreading. Adhesion to fibrinogen immobilized at low density leads to rapid increases in cytosolic Ca 2؉ and sequential formation of filopodia and lamellipodia. In contrast, adhesion to high-density fibrinogen results in transient or no increases in Ca 2؉ and simultaneous formation of filopodia and lamellipodia. ␣IIb␤3 receptors at the basal surface of platelets engage fibrinogen in a ringlike pattern at the cell edges under both conditions. This engagement is, however, more dynamic and easily reversed on high-density fibrinogen. Src and Rac activity and actin polymerization are important for adhesion to low-density fibrinogen, whereas PKC/PI3 kinases contribute to platelet spreading on highdensity fibrinogen. We conclude that 2 fundamentally different signaling mecha- IntroductionIntegrins mediate interaction of cells with their environment, responding to activation and inhibition signals (inside-out signaling) and transmitting information initiated by ligand-receptor interaction into the cell (outside-in signaling). These interactions are vital to gene regulation, cell migration, cell proliferation, and cellular differentiation in many processes. 1 The most abundant platelet integrin, ␣IIb␤3, and its signaling have been extensively studied using both platelets and other cell model systems. 2 Structural data on this integrin and the related integrin ␣V␤3 have brought new insights into its function, especially with the regard to ligand binding. 3,4 ␣IIb␤3-mediated platelet adhesion to fibrinogen has been implicated in a number of different physiologic and pathologic processes. After vascular injury, fibrinogen binds to the damaged surface and may act as one of the proteins to which platelets adhere. 5 This is of particular significance, since ␣IIb␤3 can bind to immobilized fibrinogen without prior platelet activation. 6,7 As fibrinogen is also present in atherosclerotic plaque, 8,9 platelet adhesion to fibrinogen may contribute to thrombus formation on ruptured or eroded plaques, or even to the atherosclerotic process itself. 10 Fibrinogen also preferentially deposits on artificial surfaces, including those used for vascular prostheses and biomaterials. Thus platelet adhesion to fibrinogen-coated surfaces is of importance in the biocompatibility of these surfaces. 11 ␣IIb␤3 interactions with immobilized fibrinogen trigger outside-in signaling followed by filopodial extensions, development of lamellipodia, and subsequent attachment and spreading (reviewed in Shattil 12 ). Platelet spreading on fibrinogen is associated with tyrosine phosphorylation of several platelet proteins including FAK, 13 Src, 13 and Syk. 14 Kinases involved in these processes have been shown to include protei...

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Section: Discussionmentioning

confidence: 99%

Ligand density dramatically affects integrin αIIbβ3-mediated platelet signaling and spreading

Jiroušková

Jaiswal

Coller

2007

Blood

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“…It was concluded that iodination as well as fluorescamine labeling can influence chromatographic and electrophoretic properties. (27)(28)(29)(30)(31)41) have reported that no effect of the presence of a label on the adsorption of proteins onto different surfaces was found. A possible preferential adsorption was determined by carrying out protein adsorption studies with a constant bulk protein concentration and a variation in the percentage of labeled protein.…”

Section: Discussionmentioning

confidence: 99%

High-performance liquid chromatography as a technique to measure the competitive adsorption of plasma proteins onto latices

Lensen¹,

Bargeman²,

Bergveld³

et al. 1984

Journal of Colloid and Interface Science

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Isotherms of human serum albumin (HSA), human immunoglobulin G (HIgG), and human fibrinogen (HFb) onto a polystyrene (PS)-latex were determined by depletion of protein in the solution, which was either followed by radioactivity measurements or by UV spectroscopy. Different adsorption isotherms for the same protein were obtained when either radioactivity measurements or UV spectroscopy was used as a detection technique. In order to obtain reliable results from competitive protein adsorption experiments, a method based on the use of high-performance liquid chromatography was developed. A strong preferential adsorption of HFb was observed when adsorption studies were carried out with mixtures of HSA, HFb, and HIgG. When adsorption studies were carried out with solutions containing HSA monomer and dimer, a strong preferential adsorption of HSA dimer was also observed.

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“…Thus, the adsorption of fibrinogen on PDMAEMA, and to some extent also on PSPMA, can be associated with attractive electrostatic interactions (though this does not explain why adsorption is higher on the nominally neutral PHEMA-co-PEG10MA than on the zwitterionic PSBMA). Early studies on the adsorption of fibrinogen on various polyelectrolyte surfaces also demonstrated high adsorption (from concentrations > 0.5 mg/mL) on those which have bulk ionexchange capacities of +0.8 meq g -1 (Schmitt et al 1983). The presence of deprotonated and hydrophobic segments in PDMAEMA at pH 7.4 might also contribute to a higher adsorption via hydrophobic interactions.…”

Section: Fouling Assaysmentioning

confidence: 99%

Charged hydrophilic polymer brushes and their relevance for understanding marine biofouling

et al. 2016

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Interaction of fibrinogen with solid surfaces of varying charge and hydrophobic—hydrophilic balance

Cited by 125 publications

References 27 publications

Ligand density dramatically affects integrin αIIbβ3-mediated platelet signaling and spreading

Ligand density dramatically affects integrin αIIbβ3-mediated platelet signaling and spreading

High-performance liquid chromatography as a technique to measure the competitive adsorption of plasma proteins onto latices

Charged hydrophilic polymer brushes and their relevance for understanding marine biofouling

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