Interaction of fibroblasts and polymer surfaces: relationship between surface free energy and fibroblast spreading

Abstract

Cell spreading and cell division rates of a transformed line of mouse lung fibroblasts were studied on various polymer surfaces in the presence of serum proteins. The bare polymer surfaces, as well as the protein-coated surfaces, were characterized by their polar (gamma ps) and dispersion (gamma ds) surface free energies. Cell spreading appeared to be dependent on the polar surface free energy. Cell spreading is low when the gamma ps of the bare surface is lower than 5 erg cm-2; marked spreading occurs when gamma ps is higher than 15 erg cm-2. A similar relationship was found between cell spreading and polarity of the protein-coated surfaces, although less pronounced than for the bare surfaces. Cell spreading appeared independent of the dispersion surface free energy. Cell division rate was the same on all surfaces tested.

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

“…This complexity in the literature is exacerbated by the fact that there are a number of methods of assessing cell-surface interaction that do not fall in the category of cell-attachment kinetics. These include measurement of cell spreading [90][91][92], cell interfacial energy [93], forces required to dislodge adherent cells [94][95][96], and use of detachment indices [97].…”

Evaluation of Cell Behaviour on Atmospheric Plasma Deposited Siloxane and Fluorosiloxane Coatings

“…Uncoated PS and tissue culture grade polystyrene (TCPS) were used as references. PS is an example of a hydrophobic surface with a low surface energy and a correspondingly relatively high contact angle of approximately 90° [22][23]. The surface of TCPS has been modified to impart an oxygen-containing surface chemistry that has a water contact angle in the range of 60°-70° [22][23][24].…”

An Assessment of Novel Biodegradable Magnesium Alloys for Endovascular Biomaterial Applications

“…The lack of cellular adhesion seen on the alloys is further supported by the low Zisman surface energy values for each alloy, which is consistent with Baier et al and van der Valk et al, who determined that materials with low surface energies show low cell attachment [31,32]. It is important to note that all tests were conducted in-vitro, which is not a true presentation of an actual implant.…”

“…Polymeric materials like poly-L-lactic acid (PLLA) have been shown to have an acceptable biocompatibility and to adapt to the conformation of the vessel geometry. Another limitation is the inability of the stent to fully expand by balloon deployment and the need for the application of heat, which is of concern to normal vessel biology [32]. Recently polylactide based polymeric stents have been developed, but data is limited to animal testing and are not widely published [33].…”

“…The corrosion rate at the cathode is reduced compared to the anode. The solid-solubility limit of a material is the extent to which an alloying element will dissolve in base materials without forming a different phase [32]. Generally, the higher the solid solubility limit the more likely the alloying element can homogenously disperse within a material.…”