Interaction of gold nanorods with cell culture media: Colloidal stability, cytotoxicity and cellular death modality

Cytotoxicity and Cellular Death Modality of Surface-Decorated Gold Nanorods against a Panel of Breast Cancer Cell Lines

“…Internalization of GNRs into the breast cancer cell lines was quantified and is demonstrated in Figure ; all cell lines show a similar percentage of cellular uptake. However, MCF-7 cells previously demonstrated a slightly higher percentage of cellular internalization than these cell lines . Our results indicate that the extent of cellular internalization is not the only factor affecting the degree of cytotoxicity; the rate of nanoparticle uptake and their interaction with other cellular components may contribute to cytotoxicity.…”

“…On the other hand, T47D revealed a low cytotoxicity towards the GNRs over the range from 48.0 to 3.0 μg/mL compared to other cell lines (Figure A,B). In our recent work, the phospholipid-GNRs demonstrated a higher IC 50 (∼2.3 μg/mL) towards MCF-7 cells than MDA-MB-231, ZR-75-1, and T47D . Variations in response of different types of breast cancer to cytotoxic chemotherapeutic agents is a known phenomenon. , It has been reported that doxorubicin and 5-fluorouracil induce different mechanisms in cancer cell death, where luminal cells repressed a large number of cell cycle regulated genes, whereas basal cell lines repressed genes that were involved in differentiation …”

“…We propose that phospholipid-decorated GNRs interact differently, and are more potent in basal-subtype cells than the luminal ones, which could be due to the interaction with other cell components that can modulate the MDA-MB-231 cells adhesion properties, thus enhancing their cytotoxicity …”

Colloidal Stability and Cytotoxicity of Polydopamine-Conjugated Gold Nanorods against Prostate Cancer Cell Lines

“…Interestingly, conjugating GNRs with PDA (GNRs-PDA and GNRs-PEG-PDA) greatly enhanced the conjugates’ colloidal stability upon mixing with cell culture medium with or without the addition of FBS. We demonstrated the crucial role of FBS in cell culture media in preserving the nanoparticles’ colloidal stability of several surface chemistries [ 30 ]. PDA-coated gold nanospheres were stable in protein-containing cell culture media; however, their stability was collapsed upon dispersion in protein-free media [ 20 ].…”

The Effect of Surface-Modified Gold Nanorods on the Early Stage of Embryonic Development and Angiogenesis: Insight into the Molecular Pathways

“…This analysis was performed to investigate changes in protein expression of regulatory factors implicated in cellular functions related to cell apoptosis and metastasis. Briefly, cultured chicken embryo fibroblasts (~1 × 10 6 cells) were seeded and treated with GNR (A) and GNR (B) (~5.5 µg/mL) and supplemented with 10% fetal bovine serum (FBS) for 48 h to enhance the colloidal stability of the nanorods [70]. The cell lysate was then collected, and an equal number of proteins were resolved in 10% SDS PAGE gel, then transferred onto PVDF membranes.…”