Interaction of human mannose-binding lectin (MBL) with Yersinia enterocolitica lipopolysaccharide

Kasperkiewicz, Katarzyna; Swierzko, Anna; Bartłomiejczyk, Marcin A.; Cedzyński, Maciej; Noszczyńska, Magdalena; Duda, Katarzyna; Michalski, Mateusz; Skurnik, Mikael

doi:10.1016/j.ijmm.2015.07.001

Cited by 16 publications

(20 citation statements)

References 48 publications

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“…It is tempting to speculate that MBL has a significant part in combating Yersinia infections. That speculation may be supported by our previous report demonstrating reactivity of MBL with cells and purified lipopolysaccharides of various mutants derived from Y. enterocolitica O:3 30 . Among our healthy controls, the frequency of primary MBL deficiency was practically the same as reported earlier for a Polish population 36 .…”

Section: Discussionsupporting

confidence: 90%

“…That speculation may be supported by our previous report demonstrating reactivity of MBL with cells and purified lipopolysaccharides of various mutants derived from Y. enterocolitica O:3. 30 Among our healthy controls, the frequency of primary MBL deficiency was practically the same as reported earlier for a Polish population. 36 Only a small proportion of individuals are considered MBL deficient, whereas a much higher proportion (A/A homozygotes) are capable of responding to an inflammatory stimulus ('acute phase') with increased synthesis.…”

Section: Discussionsupporting

confidence: 82%

“…As complement activation has a major role in antimicrobial defence, its malfunction could contribute to the pathogenesis of some infection‐associated arthropathies. Recently, we reported reactivity of the lectin pathway‐specific pattern recognition molecule, MBL, with cells (as well as endotoxins) of various mutants derived from Y. enterocolitica O:3, and subsequent complement activation 30 . We hypothesized that failure of the lectin pathway to contain Yersinia infections adequately could contribute to the development of JIA.…”

mentioning

confidence: 99%

“…Recently, we reported reactivity of the lectin pathway-specific pattern recognition molecule, MBL, with cells (as well as endotoxins) of various mutants derived from Y. enterocolitica O:3, and subsequent complement activation. 30 We hypothesized that failure of the lectin pathway to contain Yersinia infections adequately could contribute to the development of JIA. While our project was in progress, Petri et al 31 published a study of lectin pathway components in JIA, comparing relatively mild with more severe disease.…”

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confidence: 99%

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Lectin pathway factors in patients suffering from juvenile idiopathic arthritis

et al. 2017

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Both complement activation and certain infections (including those with Yersinia sp.) may contribute to the pathogenesis of juvenile idiopathic arthritis (JIA). We investigated factors specific for the lectin pathway of complement: mannose-binding lectin (MBL), ficolins and MBL-associated serine protease-2 (MASP-2), in 144 patients and 98 controls. One hundred and six patients had oligoarticular disease and 38 had polyarticular disease. In 51 patients (out of 133 tested), Yersinia-reactive antibodies were found (JIA Ye group). MBL deficiency was significantly more frequent in the JIA Ye group than in patients without Yersinia-reactive antibodies or in controls. Median serum ficolin-2 level was significantly lower (and proportion of values deemed ficolin-2 insufficient greater) in JIA patients irrespective of their Yersinia antibody status. The minority (C) allele at -64 of the FCN2 gene was less frequent among JIA patients than among control subjects. No differences were found in the frequency of FCN3 gene +1637delC or MASP2 +359 A>G mutations nor for median values of serum ficolin-1, ficolin-3 or MASP-2. However, high levels of serum ficolin-3 were under-represented in patients, in contrast to MBL. MBL, ficolin-1, ficolin-2, ficolin-3 and MASP-2 were also readily detectable in synovial fluid samples but at a considerably lower level than in serum. Our findings suggest a possible role for the lectin pathway in the pathogenesis of JIA, perhaps secondary to a role in host defence, and indicate that investigations on the specificity of lectin pathway recognition molecules towards specific infectious agents in JIA might be fruitful.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 82%

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confidence: 99%

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confidence: 99%

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Lectin pathway factors in patients suffering from juvenile idiopathic arthritis

et al. 2017

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“…The complement cascade can be activated by the classical mannose-binding lectin, as well as alternative pathways [59]. Lipid A, core, and O antigen activate one or more of these pathways but bacteria expressing long O antigen chains are usually more resistant to serum than their O antigen-deficient isogenic mutants [60][61][62][63][64][65][66]. However, specific chain lengths of O antigen have been shown to be important in conferring resistance [67][68][69][70][71][72][73].…”

Section: Lps Stimulates and Inhibits Host-mediated Bacterial Defencesmentioning

confidence: 99%

The Role of Pseudomonas aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology

2019

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The major constituent of the outer membrane of Gram-negative bacteria is lipopolysaccharide (LPS), which is comprised of lipid A, core oligosaccharide, and O antigen, which is a long polysaccharide chain extending into the extracellular environment. Due to the localization of LPS, it is a key molecule on the bacterial cell wall that is recognized by the host to deploy an immune defence in order to neutralize invading pathogens. However, LPS also promotes bacterial survival in a host environment by protecting the bacteria from these threats. This review explores the relationship between the different LPS glycoforms of the opportunistic pathogen Pseudomonas aeruginosa and the ability of this organism to cause persistent infections, especially in the genetic disease cystic fibrosis. We also discuss the role of LPS in facilitating biofilm formation, antibiotic resistance, and how LPS may be targeted by new antimicrobial therapies.

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Rapid Detection of Gram-Positive and -Negative Bacteria in Water Samples Using Mannan-Binding Lectin-Based Visual Biosensor

et al. 2022

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Waterborne bacterial infection is a health threat worldwide, making accurate and timely bacteria detection crucial to prevent waterborne disease outbreaks. Inspired by the intrinsic capability of mannan-binding lectin (MBL) in recognizing the pathogen-associated molecular patterns (PAMPs), a visual biosensor is developed here for the on-site detection of both Gram-positive and -negative bacteria. The biosensor was synthesized by immobilization of the MBL protein onto the blue carboxyl-functionalized polystyrene microparticles (PSM), which is then used in a two-step assay to detect bacterial cells in water samples. The first step involved a 20 min incubation following the MBL-PSM and calcium chloride solution addition to the samples. The second step was to add ethanol to the resultant blue mixture and observe the color change with the naked eye after 15 min. The biosensor had a binary (all-or-none) response, which in the presence of bacterial cells kept its blue color, while in their absence the color changed from blue to colorless. Testing the water samples spiked with four Gram-negative bacteria including Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa and two Gram-positive bacteria of Enterococcus faecalis and Staphylococcus aureus showed that the biosensor could detect all tested bacteria with a concentration as low as 101.5 CFU/ml. The performance of biosensor using the water samples from a water treatment plant also confirmed its capability to detect the pathogens in real-life water samples without the need for instrumentation.

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Interaction of human mannose-binding lectin (MBL) with Yersinia enterocolitica lipopolysaccharide

Cited by 16 publications

References 48 publications

Lectin pathway factors in patients suffering from juvenile idiopathic arthritis

Lectin pathway factors in patients suffering from juvenile idiopathic arthritis

The Role of Pseudomonas aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology

Rapid Detection of Gram-Positive and -Negative Bacteria in Water Samples Using Mannan-Binding Lectin-Based Visual Biosensor

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