Interaction of Hydralazine with Human Serum Albumin and Effect of β-Cyclodextrin on Binding: Insights from Spectroscopic and Molecular Docking Techniques

Bolattin, Mallavva B.; Nandibewoor, Sharanappa T.; Joshi, Shrinivas D.; Dixit, Sheshagiri R.; Chimatadar, Shivamurti A.

doi:10.1021/acs.iecr.6b00517

Cited by 70 publications

(30 citation statements)

References 51 publications

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“…HSA contains a tryptophan that is excited in the ultraviolet region to emit fluorescence, forming a fluorescent spectrum. When other molecules interact with HSA, tryptophan fluorescence may change, depending on the effect of this interaction on protein conformation [27,28]. HSA and MTO, CHP NPs, and MTO-CHP NPs were mixed and reacted at a molecular ratio of 3.6 : 1 (HSA: 1 5 × 10 −5 mol/L).…”

Section: Characterization Of the Interaction Between Hsa And Drug-loamentioning

confidence: 99%

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Interaction of Mitoxantrone-Loaded Cholesterol Modified Pullulan Nanoparticles with Human Serum Albumin and Effect on Drug Release

Yuan

Guo

Zhong

et al. 2019

Journal of Nanomaterials

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To clarify nanoparticle-protein interaction and their action characteristics, the interactions between MTO-CHP NPs and human serum albumin (HSA) were studied by isothermal titration calorimetry (ITC), fluorescence spectroscopy, dynamic light scattering (DLS), and circular dichroism spectroscopy (CD). Hydrophobically modified pullulan (CHP) nanoparticles (NPs) loaded with mitoxantrone (MTO) were prepared (MTO-CHP NPs) with size 166.9 nm. The spherical shape was verified by transmission electron microscopy (TEM). The ITC results demonstrated an interaction between MTO-CHP NPs mainly by hydrophobic interaction force, electrostatic force, and hydrogen bonding. The mean binding constant KA was 0.832×104 M−1 and mean HSA coverage 0.939±0.302. MTO-CHP NPs could quench the fluorescence intensity of HSA, which gradually decreased to be balanced in 9 h and indicated the completion of the complexation. The size and zeta potential changes of the combined particle were dynamically detected with DLS at 0, 3, 6, 9, 12, 15, and 18 h. When the reaction was completed at 9 h, the particle size and potential remained stable, accompanied by a size change from 89.91 to about 145 nm and potential change from -15 to -3 mV, respectively. The results of CD measurement showed that the change in ellipticity of HSA at 208 nm was similar to the fluorescence spectra and DLS measurements with MTO-CHP NPs combined with HSA. At the beginning of the reaction, the proportion of α-helix was 52.3% to 43.7%, which decreased by 39.1% at compound stabilization. The release of MTO from MTO-CHP NPs at pH=5.6 was significantly accelerated, whereas that of MTO from HSA-MTO-CHP NPs was significantly reduced, and the drug release was significantly slowed down even under acidic conditions, which indicates the beneficial effect of HSA on the persistence and stability of the HSA-MTO-CHP NP compound.

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Section: Characterization Of the Interaction Between Hsa And Drug-loamentioning

confidence: 99%

“…The maximum changes in fluorescence intensity with recombination time were recorded. The tryptophan chromophore in the HSA molecule was excited at 280 nm [28], and the emission spectrum was recorded at 290-450 nm. The excitation and emission slit widths were 5 and 12 nm, respectively.…”

Section: Characterization Of the Interaction Between Hsa And Drug-loamentioning

confidence: 99%

Interaction of Mitoxantrone-Loaded Cholesterol Modified Pullulan Nanoparticles with Human Serum Albumin and Effect on Drug Release

Yuan

Guo

Zhong

et al. 2019

Journal of Nanomaterials

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“…Sample probes of equal volume but different concentrations were placed onto the sensor, and luminescence intensity changes were recorded using a fluorescence spectrometer. The relationship between the luminous intensity of the sensing film and CrO 4 2concentration was described by the Stern-Volmer equation, I 0 /I = 1 + K SV [CrO 4 2-] [19,20], where I 0 and I are luminescence intensities in the absence and presence of CrO 4 2-, respectively. The mechanism of CrO 4 2--induced luminescence quenching was studied using an Agilent Cary UV-8000 spectrophotometer (Timme, China).…”

Section: Sensing Properties Of Thin Film Of Nanosheetsmentioning

confidence: 99%

Luminescent sensing film based on sulfosalicylic acid modified Tb(III)-doped yttrium hydroxide nanosheets

Yang

Zheng

et al. 2018

J Adv Ceram

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Sulfosalicylic acid (SSA) was used as an intercalation agent and an excellent antenna to synthesize layered rare-earth hydroxide (LRH) materials and directly obtain SSA-modified terbium-doped ytterbium hydroxide nanosheets by mechanical exfoliation. The crystal structure and morphologies of the LRHs and nanosheets were determined by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The particle size and zeta potential of the prepared nanosheets were also analyzed. The as-prepared nanosheets exhibited excellent luminescent properties. The positively charged nanosheets were electrophoretically deposited on a conductive glass to form a thin film. The luminescence of this thin film can be quenched by chromate (CrO 4 2-) and bilirubin (BR), which shows good sensing properties. The quenching mechanism of the sensing film by CrO 4 2and BR was discussed based on the spectra and structure of the film.

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“…where C p is the molar concentration of the protein, n is the number of amino acid residues in the protein (585 for HSA), and l is the path length in mm. The MRE is then converted to the α-helical content by the following equation: where 4000 is the MRE of the β-form or random coil conformations at 208 nm, and 33000 is the MRE of a pure α-helical conformation at 208 nm (Bolattin et al 2016).…”

Section: General Conclusionmentioning

confidence: 99%

“…1.37 ± 1.07 × 10 3 -0.0126 ± 0.0017 0.942 Figure A33: Representative absorbance spectra of 10 μM HSA with no PFOA, 10 μM PFOA, and 100 μM PFOA. To correct for the inner filter effect, the following equation (Bolattin et al 2016) is used:…”

Section: Individual Association Constants Of Emerging Pfass With CD Dmentioning

confidence: 99%

The Cyclodextrin-Perfluorinated Surfactant Host-Guest Complex: Fundamental Studies for Potential Environmental Remediation and Therapeutic Applications

Errico¹

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When experiments failed, or when vi papers were published, through the good and bad, they were always there to commiserate or celebrate with, and they will always be some of my best friends. Finally, I would like to thank my family: my husband Michael, for helping me stay strong; my sister Babs, for cheering me up; and especially my parents, for all their love, support, and encouragement. They always knew I would finish, and their belief in me motivated me more than they know.

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Interaction of Hydralazine with Human Serum Albumin and Effect of β-Cyclodextrin on Binding: Insights from Spectroscopic and Molecular Docking Techniques

Cited by 70 publications

References 51 publications

Interaction of Mitoxantrone-Loaded Cholesterol Modified Pullulan Nanoparticles with Human Serum Albumin and Effect on Drug Release

Interaction of Mitoxantrone-Loaded Cholesterol Modified Pullulan Nanoparticles with Human Serum Albumin and Effect on Drug Release

Luminescent sensing film based on sulfosalicylic acid modified Tb(III)-doped yttrium hydroxide nanosheets

The Cyclodextrin-Perfluorinated Surfactant Host-Guest Complex: Fundamental Studies for Potential Environmental Remediation and Therapeutic Applications

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