Interaction of hydrogen peroxide with superoxide dismutase from erythrocytes

Symonyan, M.A.; Nalbandyan, R.M.

doi:10.1016/0014-5793(72)80667-7

Cited by 60 publications

(34 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on previous reports (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(39)(40)(41)(42) and on the data presented in our study, the following pathogenetic events ofM. pneumoniae infection are suggested (Fig.…”

Section: Discussionsupporting

confidence: 69%

Role of superoxide anion in host cell injury induced by mycoplasma pneumoniae infection. A study in normal and trisomy 21 cells.

Almagor¹,

Kahane²,

Yatziv³

1984

J. Clin. Invest.

View full text Add to dashboard Cite

Abstract. The role ofMycoplasma pneumoniaegenerated superoxide and hydrogen peroxide in inducing host cell injury was studied in normal and trisomy 21 human cells. As a result of M. pneumoniae infection, catalase activity in infected normal skin fibroblasts and ciliated epithelial cells decreased by 74-77% as compared with uninfected controls. Addition of superoxide dismutase to the infected cultured cells totally prevented the inhibition whereas addition of catalase or catalytically inactivated superoxide dismutase had no protective effect. Trisomy 21 erythrocytes and cultured skin fibroblasts in which CuZn-superoxide dismutase content is 50% greater than in normal cells were infected by M. pneumoniae. The inhibition of catalase activity in these cells was 7-33% and 0-20.5%, respectively, as compared with 65-72% and 48-68% inhibition in normal infected controls. Following M. pneumoniae infection, the levels of malonyldialdehyde, an indicator for membrane lipid peroxidation were raised in trisomy 21 cultured fibroblasts by 10-32% while in normal cells malonyldialdehyde increased by 140-870%. Externally added superoxide dismutase, but not catalase, reduced the extent of lipid peroxidation in normal infected cells. Lactate dehydrogenase release from normal infected cells was time correlated with the increase in their malonyldialdehyde formation. It is suggested that superoxide generated during M. pneumoniae infection is involved in the inhibition of host cell catalase activity. The inactivation of this cellular antioxReceivedfor publication 9 August 1983 and in revisedform 24 October 1983. idative defense mechanism results in progressive oxidative damage to the M. pneumoniae-infected cells.

show abstract

Section: Discussionsupporting

confidence: 69%

Role of superoxide anion in host cell injury induced by mycoplasma pneumoniae infection. A study in normal and trisomy 21 cells.

Almagor¹,

Kahane²,

Yatziv³

1984

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Also, H 2 O 2 inhibits thiolate dependent enzymes by oxidation of their active-site thiolate moieties [34]. Similarly, SOD activity was inhibited by H 2 O 2 associated with the reduction of the active site of SOD (Cu 2+ to Cu+), and/or the structural alteration of SOD that restricts access to Cu 2+ [35,36].…”

Section: Cytoprotection and Antioxidant Activitymentioning

confidence: 99%

Phytochemical Composition of Extracts Prepared from Dietary Cucurbits and their Cytoprotective Efficacies against Hydrogen Peroxide Induced Oxidative Stress in GI Cell-INT407

Ahmad¹,

Irshad²,

Zafaryab³

et al. 2017

J Plant Biochem Physiol

View full text Add to dashboard Cite

“…However, there has been no prior explanation for the importance of HCO 3 Ϫ in this system. Therefore, in the present study, we evaluate the role of HCO 3 Ϫ in the peroxidase function of CuZn-* This work was supported by National Institutes of Health Grants HL-38324 and HL-52315 and a grant-in-aid from the American Heart Association (to J. L. Z.).…”

mentioning

confidence: 99%

Bicarbonate Is Required for the Peroxidase Function of Cu,Zn-Superoxide Dismutase at Physiological pH

Sankarapandi¹,

Zweíer²

1999

Journal of Biological Chemistry

View full text Add to dashboard Cite

Cu,Zn-superoxide dismutase (SOD1) acts as a peroxidase in the presence of H 2 O 2 at high pH (pH > 9). The high pH species of H 2 O 2 , HO 2 ؊ , was previously implicated as the reactive species. However, recent EPR studies of the enzyme performed in the physiological pH range 7.4 -7.6 with the spin trap 5,5-dimethyl-1-pyrolline-Noxide attributed the intense EPR signal of 5,5-dimethyl-1-pyrolline-N-oxide-OH obtained from SOD1 and H 2 O 2 to the peroxidase activity of the enzyme. The present study establishes that this intense signal is obtained only in the presence of bicarbonate.

show abstract

Interaction of hydrogen peroxide with superoxide dismutase from erythrocytes

Cited by 60 publications

References 11 publications

Role of superoxide anion in host cell injury induced by mycoplasma pneumoniae infection. A study in normal and trisomy 21 cells.

Role of superoxide anion in host cell injury induced by mycoplasma pneumoniae infection. A study in normal and trisomy 21 cells.

Phytochemical Composition of Extracts Prepared from Dietary Cucurbits and their Cytoprotective Efficacies against Hydrogen Peroxide Induced Oxidative Stress in GI Cell-INT407

Bicarbonate Is Required for the Peroxidase Function of Cu,Zn-Superoxide Dismutase at Physiological pH

Contact Info

Product

Resources

About