Interaction of
            <i>Cryptosporidium hominis</i>
            and
            <i>Cryptosporidium parvum</i>
            with Primary Human and Bovine Intestinal Cells

Hashim, Amna; Mulcahy, Grace; Bourke, Billy; Clyne, Marguerite

doi:10.1128/iai.74.1.99-107.2006

Cited by 56 publications

(54 citation statements)

References 31 publications

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“…Our data show sporozoites becoming engulfed by the host cell apical membrane. This phenomenon was proposed (Perkins et al 1999 ;Elliott and Clark, 2000 ;Pollok et al 2003 ;Chen et al 2004Chen et al a, b, 2005Hashim et al 2006) and recently confirmed by Valigurova et al (2008). It has been suggested that the parasite induces the host cell to encapsulate itself with the host cell apical membrane (Borowski et al 2008) to escape the hosts defence mechanisms.…”

Section: Trophozoites On the Host Cell Surface 48 H Post-inoculationmentioning

confidence: 80%

Morphological characterization ofCryptosporidium parvumlife-cycle stages in anin vitromodel system

et al. 2009

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S U M M A R YCryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages of C. parvum and their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect of C. parvum on host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease.

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Section: Trophozoites On the Host Cell Surface 48 H Post-inoculationmentioning

confidence: 80%

Morphological characterization ofCryptosporidium parvumlife-cycle stages in anin vitromodel system

et al. 2009

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“…The culture of primary human intestinal epithelial cells (PECs) that retain human characteristics would be an alternative for studying human intestinal infections. Short-term-cultured PECs have been used to study cryptosporidiosis (12). However, cultured PECs are short-lived cells and undergo apoptosis when cultured in vitro (13).…”

mentioning

confidence: 99%

Human Primary Intestinal Epithelial Cells as an Improved In Vitro Model for Cryptosporidium parvum Infection

et al. 2013

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The study of human intestinal pathogens has been limited by the lack of methods for the long-term culture of primary human intestinal epithelial cells (PECs). The development of infection models with PECs would allow a better understanding of hostparasite interactions. The objective of this study was to develop a novel method for prolonged in vitro cultivation of PECs that can be used to study Cryptosporidium infection. We isolated intact crypts from human intestines removed during weight loss surgery. The fragments of intestinal layers were cultivated with culture medium supplemented with growth factors and antiapoptotic molecules. After 7 days, the PECs formed self-regenerating cell clusters, forming villi that resemble intestinal epithelium. The PECs proliferated and remained viable for at least 60 days. The cells expressed markers for intestinal stem cells, epithelial cells, and mature enterocytes. The PECs were infected with Cryptosporidium. In contrast to older models in which parasite numbers decay, the burden of parasites increased for >120 h. In summary, we describe here a novel method for the cultivation of self-regenerating human epithelial cells from small intestinal crypts, which contain both intestinal stem cells and mature villus cells. We present data that suggest these cells support Cryptosporidium better than existing cell lines. PECs should provide an improved tool for studying host-parasite interactions involving Cryptosporidium and other intestinal pathogens.

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“…Globally, Cryptosporidium is responsible for the majority of gastrointestinal parasitic infections, representing a significant cause of morbidity and mortality in its hosts (35,53). There are currently 16 recognized species of Cryptosporidium (54)(55)(56)(57), and cryptosporidiosis in humans and livestock is mainly caused by the zoonotic pathogen Cryptosporidium parvum and the anthroponotic pathogen C. hominis (21). Cryptosporidiosis in humans usually results in self-limited watery diarrhea in immunocompetent subjects but has far more devastating effects on immunocompromised patients, e.g., AIDS patients, and can be life-threatening due to dehydration caused by chronic diarrhea (10,11).…”

mentioning

confidence: 99%

“…Cryptosporidium oocysts are highly resistant to chlorination (36) and can survive for several months in standing water (38,52), rendering them a serious problem for large-scale water suppliers and users of private water supplies. The recognition of Cryptosporidium as an important pathogen and the global increase in immunocompromised populations have resulted in a strong demand for sensitive and reliable detection and typing systems for both clinical and environmental water samples (21,32,51). This demand is further highlighted because different species of Cryptosporidium oocysts are morphologically indistinguishable from each other (15).…”

mentioning

confidence: 99%

Rapid and Sensitive Detection of Single Cryptosporidium Oocysts from Archived Glass Slides

Sunnotel¹,

Snelling²,

Xiao

et al. 2006

J Clin Microbiol

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In this study we report on the development and application of a novel method for efficiently extracting and detecting single Cryptosporidium oocysts from archived glass slides. Laser capture microscopy was used to extract low numbers of oocysts from archived glass slides. Highly sensitive real-time PCR methods were then developed to enable the rapid detection and identification of Cryptosporidium oocysts from these samples. The method was applied to fecal smears stained with a variety of standard oocyst stains and water samples. This application, with samples derived from both public health and water service laboratories, highlighted the strong potential of this method to be used as a rapid high-throughput screening tool for the routine monitoring of Cryptosporidium and other medically important pathogens from clinical, veterinary, and environmental water samples. Importantly, the application of our protocol could be used to type Cryptosporidium and other pathogens from stored archived glass slides in public health and water service laboratories, providing vital epidemiological updates and helping to identify and trace pathogens and their routes of infection and ultimately improve their control.

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Interaction of Cryptosporidium hominis and Cryptosporidium parvum with Primary Human and Bovine Intestinal Cells

Abstract: Cryptosporidiosis in humans is caused by the zoonotic pathogen

Cited by 56 publications

References 31 publications

Morphological characterization ofCryptosporidium parvumlife-cycle stages in anin vitromodel system

Morphological characterization ofCryptosporidium parvumlife-cycle stages in anin vitromodel system

Human Primary Intestinal Epithelial Cells as an Improved In Vitro Model for Cryptosporidium parvum Infection

Rapid and Sensitive Detection of Single Cryptosporidium Oocysts from Archived Glass Slides

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