Interaction of Integrin Receptors with Extracellular Matrix is Involved in Trophoblast Giant Cell Migration in Bovine Placentomes

Pfarrer, Christiane; Hirsch, Pierre; Guillomot, Michel; Leiser, Rudolf

doi:10.1016/s0143-4004(03)00059-6

Cited by 79 publications

(86 citation statements)

References 47 publications

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“…In goats, sheep, and cattle, constituents of uterine histotroph such as interferon gamma-inducible protein 10 (CXCL10), galactoside-binding, soluble, 15 (LGALS15), and insulin-like growth factor-binding protein 1 (IGFBP1) have been characterized to activate ITGs through their RGD domain during the trophectoderm attachment period (Nagaoka et al 2003, Farmer et al 2008, Simmons et al 2009). In the bovine species, the expression of ITGs has been characterized at the uteroplacental interface during the periods of trophectoderm attachment (MacLaren & Wildeman 1995, MacIntyre et al 2002 and placentation (Pfarrer et al 2003). ITGs characterized in the stages of bovine trophoblast giant cell migration and fusion with the uterine epithelial cells consist of five a-subunits (ITGA2B, ITGA3, ITGA5, ITGA8, and ITGAV) and two b-subunits (ITGB1 and ITGB3) (Pfarrer 2006).…”

Section: Introductionmentioning

confidence: 99%

Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium

Bai

Kuse

et al. 2014

Reproduction

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Following bidirectional communication, the conceptus and the uterine epithelium must establish a proper cell-cell interaction, resulting in the progression of implantation processes. To clarify the mechanism of conceptus attachment to the uterine endometrium, we studied whether vascular cell adhesion molecule (VCAM1) was expressed in bovine conceptuses or endometrium during the peri-attachment period. Uterine VCAM1 expression was minimal in day 17 (day 0Zday of estrus) cyclic and pregnant animals, but increased between days 20 and 22 of pregnancy. In the intercaruncular regions, VCAM1 protein was localized to the luminal and glandular epithelia, whereas in the caruncular regions, VCAM1 protein was detected in the stroma and endothelia of the uterine endometrium. In cultured endometrial epithelial cells (EECs), VCAM1 expression was up-regulated when treated with uterine flushings or growth factor and further increased when EECs were cocultured with bovine trophoblast CT1 cells. VCAM1 expression in CT1 cells was also up-regulated with the use of uterine flushings, and further increased when these cells were cocultured with EECs. Expression of VCAM1 receptor, integrin a 4 (ITGA4) mRNA, increased significantly in day 22 conceptuses. In day 22 pregnant uteri, VCAM1 protein was found in both EECs and conceptuses, but ITGA4 was localized only to trophoblasts. These observations indicate that cell-cell interactions between conceptuses and uterine epithelial cells are required for sufficient VCAM1 and ITGA4 expression in the bovine species and suggest that uterine VCAM1 and conceptus ITGA4 play a role in the establishment of conceptus adhesion to the uterine endometrium.

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Section: Introductionmentioning

confidence: 99%

Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium

Bai

Kuse

et al. 2014

Reproduction

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“…The success of implantation relies on several essential steps including the adjustment of the uterine environment to support the development of the conceptus and the profound remodeling of the endometrium structure necessary for the apposition, adhesion, and invasion phases (36). In contrast to human and rodents, the invasion of the maternal tissue by the fetal tissue is very limited in ruminants (71) and leads to a synepitheliochorial placentation (86). Since the trophoblast appears to be intrinsically invasive in mammals (11), apposition, adhesion, and invasion processes are thought to be controlled by the endometrium (83).…”

mentioning

confidence: 99%

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

Mansouri-Attia

Aubert

Reinaud

et al. 2009

Physiological Genomics

153

148

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At implantation the endometrium undergoes modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular (C) and the intercaruncular (IC) areas in cattle. Using a 13,257-element bovine oligonucleotide array, we established expression profiles at day 20 of the estrous cycle or pregnancy (implantation), revealing 446 and 1,295 differentially expressed genes (DEG) in C and IC areas, respectively (false discovery rate ϭ 0.08). The impact of the conceptus was higher on the immune response function in C but more prominent on the regulation of metabolism function in IC. The C vs. IC direct comparison revealed 1,177 and 453 DEG in cyclic and pregnant animals respectively (false discovery rate ϭ 0.05), with a major impact of the conceptus on metabolism and cell adhesion. We selected 15 genes including C11ORF34, CXCL12, CXCR4, PLAC8, SCARA5, and NPY and confirmed their differential expression by quantitative RT-PCR. The cellular localization was analyzed by in situ hybridization and, upon pregnancy, showed gene-specific patterns of cell distribution, including a high level of expression in the luminal epithelium for C11ORF34 and MX1. Using primary cultures of bovine endometrial cells, we identified PTN, PLAC8, and CXCL12 as interferon-(IFNT) target genes and MSX1 and CXCR7 as IFNT-regulated genes, whereas C11ORF34 was not an IFNT-regulated gene. Our transcriptomic data provide novel molecular insights accounting for the biological functions related to the C or IC endometrial areas and may contribute to the identification of potential biomarkers for normal and perturbed early pregnancy.transcriptome; interferon-tau; pregnancy; cattle IN MAMMALS, the establishment and maintenance of pregnancy require a subtle and tightly regulated communication between the conceptus (embryo and embryonic annexes) and the maternal environment (85). The success of implantation relies on several essential steps including the adjustment of the uterine environment to support the development of the conceptus and the profound remodeling of the endometrium structure necessary for the apposition, adhesion, and invasion phases (36). In contrast to human and rodents, the invasion of the maternal tissue by the fetal tissue is very limited in ruminants (71) and leads to a synepitheliochorial placentation (86). Since the trophoblast appears to be intrinsically invasive in mammals (11), apposition, adhesion, and invasion processes are thought to be controlled by the endometrium (83). In mammalian species presenting an invasive implantation, decidua restrains the invasion of the embryo in a spatiotemporal manner (20). The expression and the regulation of some factors involved in the apposition, adhesion, and invasion aspects of implantation have been reported in ruminants (62, 79), but, overall, the comparative cascade of molecular mechanisms remains largely unknown.The sequence of events occ...

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“…An apparented type of event has been described in the bovine, with trophoblast giant cells, migration into the endometrium and fusion with uterine epithelial cells [77]. Keeping in mind that the peculiarities of each species do not preclude a large common set of metabolic events leading to ROS production in ruminant animals, women or rodents, the numerous results obtained in the monogastric species give us some general clues on the ROS flux origins and effects.…”

Section: Interest Of Ros Phenomena In the Gestating Mammal: Implantationmentioning

confidence: 99%

Gestation linked radical oxygen species fluxes and vitamins and trace mineral deficiencies in the ruminant

2006

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-In mammals, radical oxygen species (ROS) are essential factors of cell replication, differentiation and growth (oxidative signal), notably during gestation, but are also potentially damaging agents. In Women, ROS play a role in remodeling of uterine tissues, implantation of the embryo, settlement of the villi and development of blood vessels characteristic of gestation. The body stores of vitamins and minerals of gestating females are used to keep ROS fluxes at a level corresponding to oxidative signals and to prevent an imbalance between their production and scavenging (oxidative stress), which would be detrimental to the mother and fetus. There is some evidence that, although based on different regulatory mechanisms, most of the effects of ROS reported in humans also occur in pregnant ruminant females, some of which have been actually reported. Many vitamins and trace elements have dual effects in the organism of mammals: (a) they are involved in the control of metabolic pathways or/and gene expression, (b) but most of the time they also display ROS trapping activity or their deficiencies induce high rates of ROS production. Deficiencies induce different disorders of gestation and can be induced by different kinds of stress. An example is given, corresponding to the decreased contents of cobalt of forages, when exposed to sustained heavy rains, so that the supply of vitamins B12 to the organism of the ruminant that grazes them is reduced and failure of gestation is induced. Outdoor exposure of ruminants to adverse climatic conditions by itself can increase the vitamin and trace element requirements. Adaptation of production systems taking into account these interactions between gestation and sources of stress or change of the quality of feeding stuffs as well as further developments of knowledge in that field is necessary to promote sustainable agricultural practices.gestation / ovine / bovine / vitamins / trace elements / antioxidant enzymes / radical oxygen species / radical phenomena

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Interaction of Integrin Receptors with Extracellular Matrix is Involved in Trophoblast Giant Cell Migration in Bovine Placentomes

Cited by 79 publications

References 47 publications

Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium

Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

Gestation linked radical oxygen species fluxes and vitamins and trace mineral deficiencies in the ruminant

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