Interaction of new butyltin citrate complex with lipid model membrane and DNA

Pruchnik, Hanna; Kral, Teresa; Hof, Martin

doi:10.1007/s10973-014-3805-4

Cited by 5 publications

(5 citation statements)

References 40 publications

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“…Samples for DSC were prepared from multilamellar liposomes (MLV) of phosphatidylcholine (DPPC) according to the procedure described previously (Pruchnik et al 2014 ). MLVs prepared from pure lecithin (control sample) and lecithin with cis -DTCEP were placed in Mettler Toledo standard aluminum crucibles of 40 μl capacity.…”

Section: Methodsmentioning

confidence: 99%

Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane

2017

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Structural properties of plasmid DNA and model lipid membrane treated with newly synthesized platinum(II) complex cis-[PtCl2{P(CH2CH2COOH)3}2] (cis-DTCEP for short) were studied and compared with effects of anticancer drug cisplatin, cis-[Pt(NH3)2Cl2] (cis-DDP for short). Time Correlated Single Photon Counting Fluorescence Correlation Spectroscopy (TCSPC-FCS) was employed to study interactions between those platinum complexes and DNA. The TCSPC-FCS results suggest that bonding of cis-DTCEP derivative to DNA leads to plasmid strain realignment towards much more compact structure than in the case of cis-DDP. Application of both differential scanning calorimetry and infrared spectroscopy to platinum complexes/DPPC showed that cis-DTCEP slightly increases the phospholipid’s main phase transition temperature resulting in decreased fluidity of the model membrane. The newly investigated compound—similarly to cis-DDP—interacts mainly with the DPPC head group however not only by the means of electrostatic forces: this compound probably enters into hydrophilic region of the lipid bilayer and forms hydrogen bonds with COO groups of glycerol and PO2 − group of DPPC.

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Section: Methodsmentioning

confidence: 99%

Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane

2017

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“…DSC studies were performed as described previously [17]. Samples for DSC were prepared from multilamellar liposomes (MLVs) of DPPC.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

“…The effect of the BTsbz on the lipid bilayer phase transition was examined mainly by the differential scanning calorimetry (DSC)-a method useful for studying the nature of biological systems such as lipids or proteins [14][15][16][17], and additionally by fluorescence and infrared spectroscopy. The above-mentioned methods have been employed to systematically monitor the influence of the BTsbz on different membrane regions.…”

Section: Introductionmentioning

confidence: 99%

Influence of cytotoxic butyltin complexes with 2-sulfobenzoic acid on the thermotropic phase behavior of lipid model membranes

Pruchnik

2016

J Therm Anal Calorim

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In the previous study, we showed that butyltin complexes with 2-sulfobenzoic acid (commonly referred to as BTsbz) exhibit a very wide biological activity. The BTsbz in vitro are very active cytotoxic agents against tumor cells-more effective than cisplatin and carboplatin (traditional anticancer drugs). These complexes are antibacterial agents against gram-positive and gram-negative bacteria, as well. The aim of the present study was to investigate the influence of BTsbz on the thermotropic phase behavior of dipalmitoylphosphatidylcholine (DPPC) model membranes. The effect of this compound on the multilamellar liposomes was studied mainly by the means of differential scanning calorimetry and additionally by the steady-state fluorimetry and infrared spectroscopy. All investigated butyltin complexes with 2-sulfobenzoic acid change the thermotropic properties of lipid model membranes: The temperature of the main phase transition of DPPC is slightly decreased, and the transition's cooperativity of the peak is very much reduced. With increasing concentration, all investigated compounds abolished the pretransition. The results suggest that BTsbz are very active in the hydrophilic area of DPPC bilayer and at the same time have an effect on membrane fluidity.

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“…The effect of extracts of SR, BS and BH on phase transition of model membrane was also examined using the fluorimetric method described in our previous work [50,51], with a few minor modifications. Samples for steady-state fluorimetry consisted of small unilamellar vesicles (SUVs) with DMPC.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Interaction of skullcap (Scutellaria baicalensis Georgi) and buckwheat (Fagopyrum esculentum Moench) extracts with lipid bilayers

Pruchnik

Włoch

Żyłka

et al. 2015

J Therm Anal Calorim

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In this paper, we report the effect of skullcap (Scutellaria baicalensis Georgi) root (SR) and buckwheat (Fagopyrum esculentum Moench) husk and stalk (BH and BS) extracts on lipid organization and fluidity of model membranes, examined by monitoring of the thermotropic phase transition of dimyristoylphosphatidylcholine (DMPC) with differential scanning calorimetry, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and fluorescence spectroscopy. Despite the very high biological activity of these extracts, such a study has not been undertaken previously. Calorimetric results on the thermotropic parameters of DMPC membranes suggest that BH and BS extracts modify them to a greater extent than SR. Fluorescence and ATR-FTIR results confirm that the compounds contained in the SR extract concentrate in the hydrophilic area of the DMPC bilayer, causing an increase in the order of the polar heads of the lipid, and they do not penetrate the hydrophobic area. Compounds contained in the BS and BH extracts also concentrate in the hydrophilic area of the bilayer; however, they partially affect the hydrophobic-hydrophilic interface of the bilayer as well as the upper part of the acyl chain region.

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Interaction of new butyltin citrate complex with lipid model membrane and DNA

Cited by 5 publications

References 40 publications

Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane

Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane

Influence of cytotoxic butyltin complexes with 2-sulfobenzoic acid on the thermotropic phase behavior of lipid model membranes

Interaction of skullcap (Scutellaria baicalensis Georgi) and buckwheat (Fagopyrum esculentum Moench) extracts with lipid bilayers

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