Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane

Abstract

Structural properties of plasmid DNA and model lipid membrane treated with newly synthesized platinum(II) complex cis-[PtCl2{P(CH2CH2COOH)3}2] (cis-DTCEP for short) were studied and compared with effects of anticancer drug cisplatin, cis-[Pt(NH3)2Cl2] (cis-DDP for short). Time Correlated Single Photon Counting Fluorescence Correlation Spectroscopy (TCSPC-FCS) was employed to study interactions between those platinum complexes and DNA. The TCSPC-FCS results suggest that bonding of cis-DTCEP derivative to DNA leads to plasmid strain realignment towards much more compact structure than in the case of cis-DDP. Application of both differential scanning calorimetry and infrared spectroscopy to platinum complexes/DPPC showed that cis-DTCEP slightly increases the phospholipid’s main phase transition temperature resulting in decreased fluidity of the model membrane. The newly investigated compound—similarly to cis-DDP—interacts mainly with the DPPC head group however not only by the means of electrostatic forces: this compound probably enters into hydrophilic region of the lipid bilayer and forms hydrogen bonds with COO groups of glycerol and PO2 − group of DPPC.

Using phosphine ligands with a biological role to modulate reactivity in novel platinum complexes

“…Complex 1 reactivity with a dsDNA has been recently studied in the presence of a model lipid membrane [ 31 ]. The results suggested that bonding of complex 1 to DNA leads to a much more compact structure than cisplatin's DNA bonding, revealing complex 1 interacts by hydrogen bonding on the hydrophilic regions.…”

“…The absence of oxygen is a very important condition for the isolation of the pure compound; otherwise, the TCEP oxidizes. Monitoring the reaction by 31 P-NMR detects a new signal at 15.9 ppm highly shifted from the free ligand signal at 56.7 ppm.…”

“…Complex 3 was also fully characterized by the usual techniques which allowed one to confirm the trans-[Pt(TCEP) 2 Cl 2 ] configuration with only one active ν(Pt-Cl) stretching band in the IR spectrum at 325 cm −1 expected for a D 2 h symmetry group. 31…”

Antiproliferative, Antimicrobial and Antiviral Activity of β-Aryl-δ-iodo-γ-lactones, Their Effect on Cellular Oxidative Stress Markers and Biological Membranes

“…In turn, a decrease in the wavenumber of the maximum of this band indicates that the lipid phosphate polar head group is located in more polar environments and that head group hydration increased [62]. Similar changes are also observed when the membrane embeds compounds having OH groups in their structure, which may also form hydrogen bonds with the phosphoric group lipids, e.g., polyphenols, plant extracts, carotenoids, complexes of platinum [61,[63][64][65][66][67]. Therefore, the band located at 1235 cm −1 (for RBCMs) shifted toward lower frequencies in the presence of lactones probably indicates an interaction via hydrogen bonds between the PO 2 -group of the lipid and lactone 1 molecules.…”

In vitro effects of the activity of novel platinum (II) complex in canine and human cell lines

“…Also, studies on the interaction of Pt‐TCEP with plasmid DNA revealed that it has a stronger folding effect than cisplatin. Additionally, it was shown that it interacts with hydrophilic region of the lipid bilayer and slightly increases the phospholipid's main phase transition temperature causing decrease of its fluidity . In our previous study, we showed that the compound had cytotoxic and pro‐apoptotic activity against canine leukaemia and lymphoma cell lines.…”