Interaction of perfluoroalkyl acids with human liver fatty acid-binding protein

Fatty Acid Binding Proteins Expressed at the Human Blood–Brain Barrier Bind Drugs in an Isoform-Specific Manner

“…Differences between K d determined by different biophysical methods are quite common, with 50% not being beyond of what is often observed. For example, the K d of the perfluoroalkyl acids, perfluorooctanoic acid and perfluorononanoic acid to FABP1 have been determined to be 2.36±0.34 μM and 1.32±0.20 μM by fluorescence displacement titrations and were found to be 6.49 μM and 3.14 μM by ITC (26). Given the higher throughput nature of the fluorescence binding assay it was selected so as to be able to screen a larger number of compounds.…”

Perfluorooctanoic acid exposure alters polyunsaturated fatty acid composition, induces oxidative stress and activates the AKT/AMPK pathway in mouse epididymis

“…In our research, the downregulated expression of KAR, ELOVL5 and FABPs genes provided evidence for the saturated PUFA decrease in the mice epididymides, except for FABP4 which showed a doseresponse increase after PFOA exposure. Increase in the transcriptional level of FABP4 might be a compensatory effect due to PFOA accumulation in organ, as PFOA displays a moderate affinity for FABP4 at a 1:1 M ratio (Sheng et al, 2016). Recognized as an energy sensor, AMPK plays an important role in energy homeostasis and nutrient metabolism by reversible phosphorylation activation at the Thr172 site (O'Neill et al, 2013).…”

Strategies for grouping per- and polyfluoroalkyl substances (PFAS) to protect human and environmental health

“…In vitro methods include, among others, equilibrium dialysis 40 and uorescence displacement. 41,42 In a recent paper, Yang et al 43 used a nontarget screening approach to identify novel PFAS present in aqueous lm forming foams (AFFF) that bind to human liver fatty acid binding protein. Computational methods are based on structure-property relationships and could potentially be used to estimate the bioaccumulation potential of novel and emerging PFAS.…”