Interaction of phagocytes with filamentous fungi

Brakhage, Axel A.; Bruns, Sandra; Thywißen, Andreas; Zipfel, Peter F.; Behnsen, Judith

doi:10.1016/j.mib.2010.04.009

Cited by 124 publications

(117 citation statements)

References 45 publications

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“…Moreover, in accordance with data in animals showing the importance of mouse NK cells for the clearance of A. fumigatus from the lungs (8), we demonstrated that human NK cells exert direct cytotoxicity against germlings; however, this is not the case with conidia. Both cytokine induction and antifungal activity suggest that NK cells perceive only germinated morphologies of A. fumigatus, recognizing that the fungus has escaped the first line of host defense, provided by alveolar macrophages and neutrophils (31)(32)(33). Priming of NK cells with rhIL-2 seems to be a prerequisite for NK cell-fungus interaction, suggesting an important role for cytokine-producing immune cells in the interplay of NK cells with A. fumigatus.…”

Section: Discussionmentioning

confidence: 99%

Human NK Cells Display Important Antifungal Activity againstAspergillus fumigatus, Which Is Directly Mediated by IFN-γ Release

Bouzani

et al. 2011

The Journal of Immunology

114

108

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Despite the strong interest in the NK cell-mediated immunity toward malignant cells and viruses, there is a relative lack of data on the interplay between NK cells and filamentous fungi, especially Aspergillus fumigatus, which is the major cause of invasive aspergillosis. By studying the in vitro interaction between human NK cells and A. fumigatus, we found only germinated morphologies to be highly immunogenic, able to induce a Th1-like response, and capable of upregulating cytokines such as IFN-γ and TNF-α. Moreover, priming NK cells with human rIL-2 and stimulating NK cells by direct NK cell–pathogen contact were essential to induce damage against A. fumigatus. However, the most interesting finding was that NK cells did not mediate anti-Aspergillus cytotoxicity through degranulation of their cytotoxic proteins (perforin, granzymes, granulysine), but via an alternative mechanism involving soluble factor(s). To our knowledge, our study is the first to demonstrate that IFN-γ, released by NK cells, directly damages A. fumigatus, attributing new properties to both human NK cells and IFN-γ and suggesting them as possible therapeutic tools against IA.

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Section: Discussionmentioning

confidence: 99%

Human NK Cells Display Important Antifungal Activity againstAspergillus fumigatus, Which Is Directly Mediated by IFN-γ Release

Bouzani

et al. 2011

The Journal of Immunology

114

108

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“…Nevertheless, the outset of different pathologies depends on the immunological status of the host (Brakhage et al 2010). As such, in healthy nonatopic individuals, the inhalation of conidia concentrations even as elevated as 10 8 conidia does not cause significant physical distress (Macher et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In these individuals, conidia are trapped in the epithelial mucus layer and are removed by coughing. Moreover, conidia that escape this first physical barrier and reach the lower respiratory tract are eliminated by residing immune effector cells such as alveolar macrophages or, if this mechanism is not sufficient owing to conidia overload, by neutrophil recruitment (Brakhage et al 2010). Both of these types of immune cells are able to generate reactive oxygen species (ROS) (Hamann et al 2008); it is a matter of debate whether ROS contribute to inactivation of conidia or act as signaling molecules (Giorgio et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide-induced secondary necrosis in conidia of Aspergillus fumigatus

et al. 2016

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Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell death. Additionally, the mechanism of H 2 O 2 -induced cell death was studied in A. fumigatus conidia for the first time. Data presented in this work reveal that the H 2 O 2 -induced conidial cell death was associated with a marked increase of TUNEL-and PI-positive cells. It is therefore suggested that conidia cell death occurs in a dose-dependent manner through a secondary necrosis mechanism. The knowledge of conidia cell death machinery may provide insights into the molecular mechanism of conidia-mediated toxicity to the respiratory tract and may pave the way for improved therapeutic approaches against A. fumigatus conidia-mediated diseases.Key words: Aspergillus fumigatus, conidia, hydrogen peroxide, secondary necrosis. Résumé :Les conidies d'Aspergillus fumigatus ont été mises en cause dans des cas d'aspergillose graves chez des patients immunocompromis. On a récemment rendu compte de l'action cytotoxique de métabolites secondaires issus de conidies d'A. fumigatus. Dans le présent ouvrage, on a adapté une méthode de détection de marqueurs de mort cellulaire chez les hyphes fongiques pour les besoins de l'analyse de cellules de conidies. En outre, on a étudié pour la première fois le mode d'induction de la mort cellulaire par le peroxyde d'hydrogène (H 2 O 2 ) chez les conidies d'A. fumigatus. Les données exposées dans le présent ouvrage révèlent que la mort cellulaire provoquée par le H 2 O 2 s'associait à une hausse appréciable des cellules positives d'après les marquages TUNEL et à l'IP. On en déduit donc que la mort cellulaire des conidies surviendrait de manière proportionnelle à la dose et découlerait d'un mécanisme de nécrose secondaire. Les connaissances lié aux rouages de la mort cellulaire des conidies pourraient laisser entrevoir le mécanisme moléculaire à l'origine de la toxicité des conidies envers les voies respiratoires et ouvriraient la voie à de nouvelles approches thérapeutiques contre les maladies provoquées par les conidies d'A. fumigatus. [Traduit par la Rédaction]

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“…Brakhage et al (2010) reported that fungi produce volatile organic compounds that contribute to ill health. Therefore the emerging and transfer of resistance genes by pathogenic fungi containing spores in food chain cannot be overemphasized.…”

mentioning

confidence: 99%

Antifungal effect of Coriolopsis polyzona (Pers) on fungi isolated from remnant foods and wastewater from restaurants in Akure metropolis, Nigeria

Ogidi¹,

Oyetayo²

2015

Afr. J. Microbiol. Res.

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Interaction of phagocytes with filamentous fungi

Cited by 124 publications

References 45 publications

Human NK Cells Display Important Antifungal Activity againstAspergillus fumigatus, Which Is Directly Mediated by IFN-γ Release

Human NK Cells Display Important Antifungal Activity againstAspergillus fumigatus, Which Is Directly Mediated by IFN-γ Release

Hydrogen peroxide-induced secondary necrosis in conidia of Aspergillus fumigatus

Antifungal effect of Coriolopsis polyzona (Pers) on fungi isolated from remnant foods and wastewater from restaurants in Akure metropolis, Nigeria

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