Interaction of poloxamine block copolymers with lipid membranes: Role of copolymer structure and membrane cholesterol content

Sandez-Macho, Isabel; Casas, M.; Lage, Emílio V.; Rial-Hermida, M. Isabel; Concheiro, Angel; Alvarez‐Lorenzo, Carmen

doi:10.1016/j.colsurfb.2015.06.019

Cited by 18 publications

(9 citation statements)

References 37 publications

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“…Tetronic 1107 is a key mediator of hyperfluorescence caused by these MPS. Tetronic 1107 forms polymeric micelles which are more stable and less damaging to the lipid bilayer than typical surfactant micelles (Sandez-Macho et al 2015). Polymeric micelles encapsulate and solubilise otherwise poorly water-soluble drugs, and block copolymers have drug delivery properties (Alakhov et al 1996;Harada & Kataoka 1998;Cammas et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment

Khan

Price

Morgan

et al. 2018

The International Journal of Biochemistry & Cell Biology

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Sodium fluorescein ('fluorescein') staining of the ocular surface is frequently an indicator of compromised ocular health, and increases in the presence of certain contact lens multi-purpose solutions (MPS), a phenomenon known as solution induced corneal staining (SICS). The mechanism(s) underpinning fluorescein hyperfluorescence are uncertain, though may reflect increased cellular uptake of fluorescein by corneal epithelial cells. We have developed an in vitro model to study fluorescein uptake in both 'generic' mammalian cells (murine fibroblasts) and human corneal cells. Fluorescein hyperfluorescence increased after treatment with two MPS associated with clinical corneal fluorescein staining, yet there was no cellular hyperfluorescence for two MPS that do not cause this staining. Increased fluorescein uptake did not correlate with presence of a necrotic or an apoptotic marker (propidium iodide and caspase-3 respectively). Incubation of MPS-treated cells with dynasore (an inhibitor of dynamin, implicated in endocytic pathways) reduced fluorescein uptake irrespective of MPS treatment. The non-ionic surfactant Tetronic 1107 (present in both MPS associated with corneal fluorescein staining) increased uptake of fluorescein for both cell types, whereas an unrelated surfactant (Triton X-100) did not. We conclude that the clinical hyperfluorescence profile observed after exposure to four MPS can be reproduced using a simple model of cellular fluorescein uptake, suggesting this is the biological basis for SICS. Fluorescein entry does not correlate with necrosis or apoptosis, but instead involves a dynamin-dependent active process. Moreover the surfactant Tetronic 1107 appears to be a key MPS constituent triggering increased fluorescein entry, and may be the major factor responsible for SICS.

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Section: Discussionmentioning

confidence: 99%

Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment

Khan

Price

Morgan

et al. 2018

The International Journal of Biochemistry & Cell Biology

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“…Lipid monolayers are a very simple but powerful tool to probe the interactions between a membrane and external compounds such as polymers. Using a monolayer made of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol, Sandez-Macho et al [161] have been able to show how different PEO-PPO-PEO copolymers with different sizes of the PEO blocks interacted with the membrane. They showed that the shorter the PEO blocks, the more the polymer expanded the surface area per lipid and increased the membrane permeability.…”

Section: Interactions With Model Membranesmentioning

confidence: 99%

Rational design of block copolymer self-assemblies in photodynamic therapy

Demazeau¹,

Gibot²,

Mingotaud³

et al. 2020

Beilstein J. Nanotechnol.

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Photodynamic therapy is a technique already used in ophthalmology or oncology. It is based on the local production of reactive oxygen species through an energy transfer from an excited photosensitizer to oxygen present in the biological tissue. This review first presents an update, mainly covering the last five years, regarding the block copolymers used as nanovectors for the delivery of the photosensitizer. In particular, we describe the chemical nature and structure of the block copolymers showing a very large range of existing systems, spanning from natural polymers such as proteins or polysaccharides to synthetic ones such as polyesters or polyacrylates. A second part focuses on important parameters for their design and the improvement of their efficiency. Finally, particular attention has been paid to the question of nanocarrier internalization and interaction with membranes (both biomimetic and cellular), and the importance of intracellular targeting has been addressed.

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“…Nos últimos anos, vários estudos vêm sendo realizados com o objetivo de averiguar os efeitos ocasionados pela estabilização de Poloxâmeros nos lipossomas (LE MEINS et al, 2013;SANDEZ-MACHO et al, 2015;KLEMETSRUD et al, 2016;PIPPA et al, 2016), uma vez que as propriedades físicas e químicas dos Poloxâmeros podem ser moduladas mediante a modificação da razão molar entre os blocos PEO e PPO, a qual varia de 1:9 até 8:2 (ALEXANDRIDIS; HOLZWARTHF; HATTON, 1994). Assim, sua interação com estruturas celulares pode ser ajustada para a obtenção de biomateriais mais adequados.…”

Section: Lipossomas Associados a Copolímeros Triblocounclassified

Lipossomas De Longa-Circulação Como Estratégia Promissora Para O Transporte E Biodisponibilização De Fármacos No Tratamento Do Câncer

et al. 2020

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Interaction of poloxamine block copolymers with lipid membranes: Role of copolymer structure and membrane cholesterol content

Cited by 18 publications

References 37 publications

Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment

Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment

Rational design of block copolymer self-assemblies in photodynamic therapy

Lipossomas De Longa-Circulação Como Estratégia Promissora Para O Transporte E Biodisponibilização De Fármacos No Tratamento Do Câncer

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