Interaction of porcine mycoplasmas with fresh animal serum

A mycoplasma designated strain R171T (type strain) was isolated from the infraorbital sinus of an adult chicken. This organism was assigned to the class Mollicutes and the order Mycoplasmutales on the basis of its morphological, physical, and cultural characteristics. Its deoxyribonucleic acid base composition was 24.5 mol% guanine plus cytosine. Strain R171T was sterol dependent, and since it did not produce helical forms or hydrolyze urea, it was assigned to the family Mycoplasmataceae and the genus Mycoplasma. Strain R171T fermented glucose, hydrolyzed arginine, and produced films and spots. This strain was shown to be serologically distinct from 74 currently accepted mycoplasma species or serovars by growth inhibition, immunofluorescence, and immunodiffusion tests, which were supported in some cases by metabolism inhibition tests. Thus, strain R171T appears to be a new and distinct mycoplasma s ecies, for which we propose the name Mycoplasma lipofaciens (= NCTC 10191' = ATCC 35015T).During a survey of mycoplasmosis in domestic poultry and wild birds, a number of isolates were recovered from the respiratory tracts of chickens; these isolates were apparently serologically distinct from previously recognized avian species (23). Particular interest was focussed on isolates which were biochemically similar to Mycoplasma iowae (previously avian serovar I) in having the ability to utilize both glucose and arginine but which showed no serological relationship to this group of mycoplasmas during preliminary tests.In view of the importance of mycoplasma detection to the poultry industry and of the increasing evidence of the pathogenicity of M . iowae (8,29,32,40,41), we thought that it would be worthwhile to characterize these strains further. The aims of this paper are to establish that one of these isolates, strain R171T (type strain), is a new Mycoplasma species and to provide a detailed description of the morphological, physical, cultural, biochemical, and serological properties of this organism in accordance with the recommendations of the International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes (22).(Preliminary results of this investigation were presented at the 3rd Conference of the International Organization for Mycoplasmology , Custer, S.D., in 1980.) MATERIALS AND METHODSMycoplasma strains. Most reference strains used in this study were obtained from the World Health OrganizatiodFood and Agriculture Organization Collaborating Centre for Animal Mycoplasmas, University of Aarhus , Denmark; exceptions were Mycoplasma sualvi, Mycoplasma faucium, Mycoplasma bovoculi, and Mycoplasma hominis reference strains, which were obtained from the National Collection of Type Cultures, London, England. Strain R171T was isolated from the infraorbital sinus of an adult chicken in 1975; the flock to which this chicken belonged was serologically positive for Mycoplasma synoviae but clinically normal.Media. For isolation and laboratory examination, strains were normally propagated in a modifi...

show abstract

“…This feature has been reported previously in some porcine mycoplasmas (33) and some ureaplasmas (21).…”

Section: Discussionsupporting

confidence: 86%

Mycoplasma lipofaciens, a New Species of Avian Origin

Bradbury¹,

Forrest²,

Williams³

1983

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…Since the mycoplasmacidal activity of whey is heat stable and dialysable it is distinct from the inhibitory activity previously described for myco-plasmas in serum and semen (Taylor-Robinson, Thomas & Dawson, 1969;Roberts, 1971). The mycoplasmacidal activity of this dialysable whey fraction is not confined to ureaplasmas, an action previously described (Brownlie et al 1974a).…”

Section: Discussionmentioning

confidence: 66%

Presence of a dialysable fraction in normal bovine whey capable of killing several species of bovine mycoplasmas

Howard

Brownlie

Gourlay

et al. 1975

J. Hyg.

View full text Add to dashboard Cite

SUMMARYMost normal bovine whey samples contain a fraction that survives heating at 560 C. for 30 min., passes through a dialysis membrane and kills a maximum of seven out of ten of the different bovine mycoplasma species tested. Some whey samples appear more active than others but not all affect the same strains of mycoplasma indicating some specificity in their action. Absorption of the active factor from whey by heterologous and homologous mycoplasmas and by erythrocytes was observed. Binding of the factor to mycoplasmas appears to be temperature-dependent and non-specific, but subsequent mycoplasmacidal action shows some specificity.

show abstract

Identification of Mycoplasma Hyorhinis

Roberts

Pijoan

1971

British Veterinary Journal

View full text Add to dashboard Cite

Interaction of porcine mycoplasmas with fresh animal serum

Cited by 6 publications

References 19 publications

Mycoplasma lipofaciens, a New Species of Avian Origin

Mycoplasma lipofaciens, a New Species of Avian Origin

Presence of a dialysable fraction in normal bovine whey capable of killing several species of bovine mycoplasmas

Identification of Mycoplasma Hyorhinis

Contact Info

Product

Resources

About