Interaction of procarbazine with calf thymus DNA—a biophysical and molecular docking study

Anwer, Razique; Ahmad, Naeem; Qumaizi, Khalid I. Al; Khamees, Osama A. Al; Shaqha, Waleed Mohammed Al; Fatma, Tasneem

doi:10.1002/jmr.2599

Cited by 13 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, the intercalation binding stabilize the DNA double helix leading to a significant rise in T m by about 5-8 °C, while in the case of non-intercalative interaction (groove binding or electrostatic binding at the DNA surface), a small change in the T m is observed. 19,20 The experimental melting curves of DNA upon addition of actinomycin D within the concentration range 0 < R < 1, where R = [ActD]/[DNA] ratios are presented in Fig. 2.…”

Section: Resultsmentioning

confidence: 99%

Effect of SDS Micelles on Actinomycin D – DNA Complexes

Toader

Dascalu

Enache

2022

ACSi

View full text Add to dashboard Cite

DNA thermal denaturation was evaluated as a measure of the effect of antitumor drug actinomycin D on the stability of the double helix and also the effect of SDS micelles on actinomycin D – DNA complexes. The results indicated that the melting temperature of DNA was dependent on drug concentration, increasing with actinomycin D concentration. High thermal stabilization (about 10 °C) of the DNA helix after the association with actinomycin D clearly demonstrates the intercalative binding mode. The presence of SDS micelles leads to the release of intercalated actinomcyin D molecules from DNA double helix and their further relocation in surfactant micelles. These results highlighted that the drug release can be controlled in time and by varying the concentration and nature of surfactant.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of SDS Micelles on Actinomycin D – DNA Complexes

Toader

Dascalu

Enache

2022

ACSi

View full text Add to dashboard Cite

show abstract

“…CD spectroscopy is commonly used to study drug DNA interactions . When any drug molecules interact with DNA, they may induce some conformational changes in the backbone of DNA .…”

Section: Resultsmentioning

confidence: 99%

Study on the interaction of antidiabetic drug Pioglitazone with calf thymus DNA using spectroscopic techniques

Qumaizi

Anwer

Ahmad

et al. 2018

J of Molecular Recognition

Self Cite

View full text Add to dashboard Cite

Pioglitazone is an antidiabetic drug used to treat type 2 diabetes mellitus. Interaction of Pioglitazone with calf thymus DNA was investigated using multispectroscopic techniques and molecular docking study. Quenching and binding constant was calculated at 3 different temperatures. The binding constant of Pioglitazone with calf thymus DNA was calculated to be 6.49 × 10 M at 293 K. The quenching mechanism was found to be a static process, and thermodynamic parameters revealed van der Waals interactions and hydrogen bonds to be the major force working in Pioglitazone-DNA interaction. Pioglitazone follows the nonintercalative mode of binding and was involved in complex formation with DNA through minor groove binding and electrostatic interactions. Experiments like KI quenching studies, dye displacement assays, Circular Dichroism (CD) spectroscopy, DNA melting study, and viscosity measurements studies supported the nonintercalative mode of binding. This was further corroborated by molecular docking studies.

show abstract

“…Some of the binding affinities are even comparable to that of their corresponding bioreceptors. [68][69][70] In contrast, the binding affinities of NT-syn to these drug molecules are significantly weaker (in the range of 500-7.7 × 10 4 M -1 ). The reason is unknown and may be due to better hydrogen bonding in the cavity of NT-anti.…”

Section: Binding To Organic Drug Moleculesmentioning

confidence: 99%

Biomimetic Recognition of Organic Drug Molecules in Water by Amide Naphthotubes

Quan

Lin

et al. 2021

CCS Chem

View full text Add to dashboard Cite

Molecular recognition in water is the basis of numerous biological functions. The key for efficient and selective recognition of an organic drug molecule is to bind both its polar and nonpolar groups. This is achieved by bioreceptors for which specific noncovalent interactions are efficiently used in a hydrophobic pocket. In contrast, most synthetic receptors cannot efficiently bind the neutral, polar groups of drug molecules and, thus, often exhibit poor binding selectivity and affinity. In this research, we report a systematic study on the binding behaviors of three types of macrocyclic hosts (amide naphthotubes, cucurbit[7]uril, and β-cyclodextrin) to 18 model compounds and 13 drug molecules. Our results show that the high desolvation penalty of polar groups of guests is the reason for the relatively low binding affinity of cucurbit[7]uril and β-cyclodextrin. However, amide naphthotubes with a biomimetic cavity bind efficiently and selectively to organic guests through hydrophobic effects and hydrogen bonding. Drug molecules with multiple polar groups can be better accommodated by these naphthotubes. The anti-configured naphthotube show good biocompatibility according to preliminary cell experiments and is capable of enhancing the water solubility of two poorly soluble drug molecules. Therefore, they may have practical applications in pharmaceutical sciences.

show abstract

Interaction of procarbazine with calf thymus DNA—a biophysical and molecular docking study

Cited by 13 publications

References 30 publications

Effect of SDS Micelles on Actinomycin D – DNA Complexes

Effect of SDS Micelles on Actinomycin D – DNA Complexes

Study on the interaction of antidiabetic drug Pioglitazone with calf thymus DNA using spectroscopic techniques

Biomimetic Recognition of Organic Drug Molecules in Water by Amide Naphthotubes

Contact Info

Product

Resources

About