Interaction of stattic, a STAT3 inhibitor with human serum albumin: spectroscopic and computational study

Affandi, Ida Syazwani M; Lee, Wei Qi; Feroz, Shevin Rizal; Mohamad, Saharuddin Bin; Tayyab, Saad

doi:10.1080/07391102.2016.1264887

Cited by 15 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…30 In plasma at pH 7.4, both S@C-PLGA NPs and S@PLGA NPs exhibited similar drug release patterns, where ~12% of Stattic was released rapidly within 2 h. This indicated rapid diffusion of Stattic from the polymeric matrix to the plasma protein, likely owing to affinity of Stattic towards albumin molecules. 31 A similar observation has been reported previously, where Stattic entrapped within a synthetic polymeric micellar carrier (P71D3) 32 was rapidly released when the drug-carriercomplexes were incubated in an albumin solution. 33 The Stattic burst release from S@C-PLGA NPs in this study was considerably low (< 15%), suggesting the suitability of C-PLGA NPs as the delivery vehicles for Stattic and antimetastatic drugs with a similar chemical structure.…”

Section: Stattic Releasing Profile In Pbs and Plasmasupporting

confidence: 81%

Chitosan-Coated-PLGA Nanoparticles Enhance the Antitumor and Antimigration Activity of Stattic – A STAT3 Dimerization Blocker

Fong¹,

Foo²,

Saw³

et al. 2022

IJN

View full text Add to dashboard Cite

The use of nanocarriers to improve the delivery and efficacy of antimetastatic agents is less explored when compared to cytotoxic agents. This study reports the entrapment of an antimetastatic Signal Transducer and Activator of Transcription 3 (STAT3) dimerization blocker, Stattic (S) into a chitosan-coated-poly(lactic-co-glycolic acid) (C-PLGA) nanocarrier and the improvement on the drug's physicochemical, in vitro and in vivo antimetastatic properties post entrapment. Methods: In vitro, physicochemical properties of the Stattic-entrapped C-PLGA nanoparticles (S@C-PLGA) and Stattic-entrapped PLGA nanoparticles (S@PLGA, control) in terms of size, zeta potential, polydispersity index, drug loading, entrapment efficiency, Stattic release in different medium and cytotoxicity were firstly evaluated. The in vitro antimigration properties of the nanoparticles on breast cancer cell lines were then studied by Scratch assay and Transwell assay. Study on the in vivo antitumor efficacy and antimetastatic properties of

show abstract

Section: Stattic Releasing Profile In Pbs and Plasmasupporting

confidence: 81%

Chitosan-Coated-PLGA Nanoparticles Enhance the Antitumor and Antimigration Activity of Stattic – A STAT3 Dimerization Blocker

Fong¹,

Foo²,

Saw³

et al. 2022

IJN

View full text Add to dashboard Cite

show abstract

“…Signal transducers and activators of transcription 3 (STAT3), a member of a family of seven proteins (STATs 1, 2, 3, 4, 5a, 5b, and 6), is involved in proliferation, angiogenesis, metastasis, and immunosuppression [1]. Interleukin 6 (IL-6) activates STAT3 via the phosphorylation of tyrosine 705 [2], while STAT3 inhibitors, such as STA-21 [3], Stattic [4,5], S31-201 [6], and BP-1-102 [7], inhibit the phosphorylation of STAT3 [8] via interaction with the STAT3 SH-2 domains.…”

Section: Introductionmentioning

confidence: 99%

Suppression of STAT3 Phosphorylation and RelA/p65 Acetylation Mediated by MicroRNA134 Plays a Pivotal Role in the Apoptotic Effect of Lambertianic Acid

Sim

Lee

Jung

et al. 2019

IJMS

View full text Add to dashboard Cite

As p300-mediated RelA/p65 hyperacetylation by signal transducers and activators of transcription 3 (STAT3) is critical for NF-κB activation, in the current study, the apoptotic mechanism of lambertianic acid (LA) was explored in relation to STAT3 phosphorylation and RelA/p65 acetylation in MCF-7, DU145, PC-3, and MDA-MB-453 cells. LA significantly increased the cytotoxicity, sub G 1 population, and the cleavage of poly (ADP-ribose) polymerase (PARP) in MDA-MB-453 or PC-3 cells (STAT3 mutant), more than in the MCF-7 or DU145 cells (STAT3 wild). Consistently, LA inhibited the phosphorylation of STAT3 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and disrupted the interaction between p-STAT3, p300, NF-κB, and RelA/p65 acetylation (Ac-RelA/p65) in the MCF-7 and DU145 cells. Also, LA reduced the nuclear translocation of STAT3 and NF-κB via their colocalization, and also suppressed the protein expression of XIAP, survivin, Bcl-2, Bcl-xL, vascular endothelial growth factor (VEGF), Cox-2, c-Myc and mRNA expression of interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α) in MCF-7 cells. Conversely, IL-6 blocked the ability of LA to suppress the cytotoxicity and PARP cleavage, while the depletion of STAT3 or p300 enhanced the PARP cleavage of LA in the MCF-7 cells. Notably, LA upregulated the level of miRNA134 and so miRNA134 mimic attenuated the expression of pro-PARP, p-STAT3, and Ac-RelA, while the miRNA134 inhibitor reversed the ability of LA to reduce the expression of Ac-RelA and pro-PARP in MCF-7 cells. Overall, these findings suggest that LA induced apoptosis via the miRNA-134 mediated inhibition of STAT3 and RelA/p65 acetylation.

show abstract

Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative marker with human serum albumin

et al. 2019

View full text Add to dashboard Cite

Interaction of stattic, a STAT3 inhibitor with human serum albumin: spectroscopic and computational study

Cited by 15 publications

References 13 publications

Chitosan-Coated-PLGA Nanoparticles Enhance the Antitumor and Antimigration Activity of Stattic – A STAT3 Dimerization Blocker

Chitosan-Coated-PLGA Nanoparticles Enhance the Antitumor and Antimigration Activity of Stattic – A STAT3 Dimerization Blocker

Suppression of STAT3 Phosphorylation and RelA/p65 Acetylation Mediated by MicroRNA134 Plays a Pivotal Role in the Apoptotic Effect of Lambertianic Acid

Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative marker with human serum albumin

Contact Info

Product

Resources

About