Interaction of the rumen fungus Orpinomyces joyonii with Megasphaera elsdenii and Eubacterium limosum

Abstract

The degradation and fermentation of microcrystalline cellulose were studied in monoculture of the polycentric anaerobic fungus Orpinomyces joyonii and in co-cultures with the rumen bacteria Megasphaera elsdenii and Eubacterium limosum. More than 25% of cellulose hydrolysis products (glucose and cellodextrins) were released by the fungus into the medium after 8 d of cultivation. These products were metabolized by bacteria in mixed cultures. In co-culture with the fungus M. elsdenii and E. limosum increased the extent of microcrystalline cellulose degradation by 10.12% and 7.96%, respectively. Biomass yield in co-cultures was increased by 89.9% and 59.4% for M. elsdenii and E. limosum. Y cellulose for fungus alone was 52.29 g dry matter mol-1 glucose. These values were 64.93 and 55.92 g mol-1 glucose unit in co-culture with M. elsdenii and E. limosum, respectively.

The Rumen Microbial Ecosystem

“…L2 were grown on glucose, although the fermentation product profiles shifted towards acetate only about 50% of the fungal hydrogen was utilized (Rees et aI., 1995). When E. limosum was co-cultured with 0. joyonii, cellulose degradation increased (Hodrova et al, 1995). Fungal Hz was utilized only poorly.…”

“…Release of lactate inhibition can account for the stimulation of fungal cellulolysis when fungi are co-cultured with Megasphaera elsdenii Hodrova et al, 1995), an important lactate-utilizing bacterium in the rumen (Chapter 2). However, the stimulating effects from M.…”

The Potential of Strategies Used to Mitigate Enteric Methane Emissions in Nutrition Studies of Ruminants: A Review

“…Howsoever, the abundance and activity of anaerobic fungi is regulated to a certain extent by some bacteria and protozoa species in the rumen. The solubility of crystalline cellulose, release of endoglucanase, and production of short chain fatty acids was reduced when chitinolytic Clostridium tertium was co-cultured with anaerobic fungi (Hodrová et al, 1995). Also, when anaerobic fungi were incubated with protozoa, it was evident that protozoa are capable to prey and digest fungi (Morgavi et al, 1994).…”

The effect of yellow affinity substance on cellulases of Ruminococcus flavefaciens

“…Medium believed to be a carotenoid-like compound of MW 1050-was boiled and after cooling 0·5 g of cysteine and 4 g of 1300. In the presence of oxygen it is easily oxidized with the Na 2 CO 3 were added (Holdeman et al 1977). loss of yellow colour (Ljungdahl et al 1988).…”