Interaction of Trace Elements and Ligninolytic Enzymes in Pleurotus eryngii

Abstract

Considering the fact that some trace elements have influence on the growth and physiological processes in the fungi, the aim of this study was to resolve the question of whether Fe, Zn, and Se, added to the medium in the different forms, affect activity of selected Pleurotus eryngii ligninolytic enzymes participating in mandarine peels submerged fermentation. Compared with the control, Fe and Zn showed stimulatory effect on laccase activity. Reduction in the activity was emphasized with cultivation time, but it was slower in the presence of Zn than in the presence of Fe. Among studied Se forms, SeO2 was the most unfavorable Se source for laccase activity. All tested trace elements, independent on form, showed stimulatory effect on the activity of Mn-oxidizing peroxidases on the seventh day of cultivation. Based on the results, it could be concluded that the presence of the tested trace elements can advance various biotechnological processes among which is biodegradation of agricultural wastes, potential environmental pollutants.

Influence of Trace Elements on Ligninolytic Enzyme Activity of Pleurotus ostreatus and P. pulmonarius

“…An increase of the activity in P. pulmonarius HAI 572 in the presence of Se is in consonance with previously published results for Bjerkendera adusta (Catal et al 2008) and P. eryngii (Stajić and Vukojević 2011). Contrary to B. adusta, in which enzyme activities were significantly inhibited at concentrations between 0.1 mM and 0.2 mM, Pleurotus species have shown higher tolerance and a significant level of Mn-oxidizing peroxidases activity at a concentration of 1 mM.…”

“…The stimulatory effect of Se on activity against phenol red oxidation in the absence of the external Mn 2+ , demonstrated in P. eryngii (Stajić and Vukojević 2011), was not proven in the studied species. One possible explanation is that the level of Mn-oxidizing peroxidases activity depends on Se influence on gene regulation.…”

Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper

“…Notably, the same metal can exert opposing effects in different species. Compared with the control, laccase activity significantly increased in the presence of Fe 2C in Pleurotus eryngii, [25] while Fe 2C markedly inhibited laccase production in Ganoderma lucidum. [26] Pleurotus ostreatus is a white-rot basidiomycete living as a saprophyte on dead or decaying wood secreting different enzymes involved in lignin degradation.…”

“…The mycelia were cultivated in potato dextrose agar (PDA) medium containing 200 g/L potato extract, 20 g/L D-glucose, 3 g/L KH 2 PO 4 , 1.5 g/L MgSO 4 and 20 g/L agar, at 25 C. After 7 days of growth, the mycelia were stored at 4 C, and the stocks were transferred to fresh PDA every three months. Liquid PDB medium (PDA medium without agar) was used in shaken cultures.…”

ICP-MS trace element analysis in serum and whole blood

“…Almost every metabolic process operates by involving trace elements, ultra-trace elements, or minerals, making them essential for human metabolism; biochemical-and metabolic interactions, very relevant to human medicine as well as in diagnostics and therapy, have already been described in the context of the role of these elements. Especially for trace elements such as Se, Fe, Zn and Cu, but also for minerals such as Na, K, Ca and Mg and other, specific, binding-and transport-proteins could be identified in different membranes of cells, intracellularly and in body fluids [1,2].…”