Interaction of vasoactive intestinal peptide with human blood mononuclear cells

Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes.

“…There was no binding on the brush border membrane of rat jejunal cells. These characteristics of 1251-VIP binding on the rabbit and rat enterocytes are comparable to those previously reported in rat and guinea pig intestinal cells (7)(8)(9), rat brain (31), guinea pig pancreas (21), and human mononuclear cells (32). Our study also confirmed previous reports that VIP stimulates adenylate cyclase activity on the basolateral membrane (5) and showed a good correlation between VIP binding and the increase in adenylate cyclase activity.…”

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

“…The PAC1-R gene is expressed in rat peritoneal macrophages but not in peritoneal lymphocytes (Delgado et al, 1996a;Pozo et al, 1997). VIP-preferring sites are present in human blood mononuclear cells (Guerrero et al, 1981) and in murine splenocytes (Tatsuno et al, 1991b). The VPAC1-R gene is constitutively expressed in T-lymphocytes and thymocytes (Waschek et al, 1995a;Delgado et al, 1996c,d;Johnson et al, 1996).…”

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

“…The last splice variant identified, PAC 1-3a , encodes a rat full-length receptor with an extra 24-amino acid cassette between exons III and IV, is coupled to both cAMP and inositol phosphate pathways, and is expressed during the spermatogenic cycle (Daniel et al, 2001 (Guerrero et al, 1981;O'Dorisio et al, 1981) by binding techniques (using 125 I-VIP as a ligand) and adenylyl cyclase enzyme measurements. Later on, VIP binding sites were identified in non adherent human peripheral mononuclear cells depleted of B lymphocytes and monocytes (Danek et al, 1983).…”

“…The K d value of the single class binding site and the high-affinity binding site is similar. For the high-affinity binding sites, the reported K d values are 0.05 nM in rat blood mononuclear cells (Calvo et al, 1986b) and 0.24 nM in human blood mononuclear cells (Guerrero et al, 1981), whereas in the case of single class affinity binding sites, K d values are 0.24 nM (Ottaway et al, 1983) and 0.47 nM (Danek et al, 1983) in human blood mononuclear cells and around 0.2 nM in spleen, Peyer's patches, and mesenteric and subcutaneous lymph node lymphocytes (Ottaway and Greenberg, 1984). Early experiments pointed out a lack of adenylyl cyclase stimulation by VIP in human monocytes (O'Dorisio et al, 1981), so it was reasoned that the low binding sites reported in human mononuclear cells (Guerrero et al, 1981) corresponded to the monocyte component (Danek et al, 1983).…”