Interaction of vasoactive intestinal peptide with rat small intestinal epithelial cells after intestinal resection

Partial (60%) resection of rat small bowel was performed in order to obtain a model of intestinal mucosal hyperplasia for studying specific insulin binding. The affinity, but not the binding capacity, of insulin receptors in the adaptive mucosa decreased three and seven days following enterectomy. This modification took place only in crypt cells but not in mature villous cells. Since plasma insulin levels were not altered by the surgical manipulation, the observed decrease of insulin binding could not be related to regulation by insulin concentration. These results do not support a trophic role of insulin on intestinal mucosa and appear to be more a consequence of the hyperactive status of proliferation and differentiation at the mucosal level.

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Insulin binding to rat intestinal epithelial cells following partial small-bowel resection

Fernández-Moreno

Arilla

Prieto

1986

Bioscience Reports

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Vento

Kiviluoto²,

Pakarinen³

et al. 1998

Digestive Diseases and Sciences

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The aim of this study was to evaluate possible changes in the neuropeptide innervation pattern of the remaining porcine ileum following 75% proximal resection of the small intestine. Three-month-old piglets were operated on and two months postoperatively full-thickness specimens of the proximal part of the distal ileum wall were taken. Age-matched 3- and 5-month-old unoperated piglets were used as controls. The number and intensity of VIP-, galanin-, enkephalin-, substance P-, and somatostatin-containing nerve fibers were estimated in sections processed for immunofluorescence microscopy and subjected to quantitative scoring. The VIP-, galanin-, and enkephalin-immunoreactive fibers of the circular muscle layer and villi were also quantitated by computer-assisted morphometry. The number and intensity of VIP-immunoreactive fibers in the mucosa and circular muscle layer markedly decreased after resection as compared to 3-month-old and 5-month-old controls (P < 0.05). The galanin immunoreactivity index decreased significantly after resection in the circular muscle layer as compared to both control groups (P < 0.05). The increase in the number of enkephalin-immunoreactive nerve fibers that normally occurred from 3 to 5 months of age was inhibited by the resection. We were not able to see any differences in somatostatin or substance P immunoreactivity between the groups. The results suggest that massive resection induces significant changes in the neuropeptide-containing innervation of the remaining small intestine. These findings are compatible with altered motor activity and mucosa function in the remain intestine.

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Interaction of vasoactive intestinal peptide with rat small intestinal epithelial cells after intestinal resection

Cited by 2 publications

References 13 publications

Insulin binding to rat intestinal epithelial cells following partial small-bowel resection

Insulin binding to rat intestinal epithelial cells following partial small-bowel resection

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