Interaction of xenobiotics with mercury-adsorbed phospholipid monolayers

Nelson, Andrew; Auffret, N.

doi:10.1016/0141-1136(88)90253-x

Cited by 5 publications

(1 citation statement)

References 8 publications

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“…The phospholipid monolayer on a mercury electrode is a classical electrochemical system for modeling biological membrane processes. − In spite of the fact that only a monolayer is being investigated, as opposed to a bilayer which forms the backbone of biological membranes, the sheer simplicity and manipulability of the model makes it a perfect vehicle for understanding the fundamental principles of biological membrane structure and function. This model system has also been shown to have a particular useful application to study the fundamental physical chemical mechanisms of ion channel function with incorporated monomolecular gramicidin in the monolayer. − …”

Section: Introductionmentioning

confidence: 99%

Interpreting Ion Fluxes to Channel Arrays in Monolayers

et al. 2007

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The exponentially decaying permeability model interprets the chronoamperometric currents arising from Tl+ reduction at a Hg electrode covered with a phospholipid monolayer (DOPC) containing gramicidin monomer by combining three processes: (i) the diffusion of an ion to a membrane surface with an array of channels, (ii) the conformational dynamics of the individual channels, and (iii) the passage of the ion through the channels. The introduction of a variable permeability allows us to uncouple the diffusion from the heterogeneous processes, given that the concentration of a species at the active surface can be obtained by semi-integration of the currents. Consideration of a reverse step for the dehydration process at the mouth of the channel allows the analysis of potential steps away from diffusion-limited conditions where a Nernstian-like behavior of the relevant parameter is observed. The model has been successfully applied to data with all trans retinol or benzo-alpha-pyrene as additive to the phospholipid monolayer and to monolayers without any additive at all.

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Section: Introductionmentioning

confidence: 99%

Interpreting Ion Fluxes to Channel Arrays in Monolayers

et al. 2007

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Organic Xenobiotic Metabolism in Marine Invertebrates

Livingstone

1991

Advances in Comparative and Environmental Physiology

252

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Polarization Modulation Infrared Reflection−Absorption Spectroscopy Studies of the Influence of Perfluorinated Compounds on the Properties of a Model Biological Membrane

et al. 2008

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A combination of the Langmuir-Blodgett and Langmuir-Schaefer techniques has been used to build a 1,2-dimyristoyl- sn-glycero-3-phosphocholine (DMPC) bilayer at a Au(111) electrode surface with hydrogen-substituted acyl chains in the top leaflet (solution side) and deuterium-substituted acyl chains in the bottom leaflet (gold side). Polarization modulation infrared reflection-absorption spectroscopy was used to determine changes in the conformation and orientation of the acyl chains of DMPC caused by the incorporation of two selected perfluorinated compounds, perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS), into the top leaflet of the bilayer. The incorporation of perfluorinated compounds into the DMPC bilayer caused a broadening of the methylene peaks and a shift in the methylene band positions toward higher frequencies. In addition, the tilt angle of the acyl chains decreased in comparison to the tilt angle of a pure DMPC bilayer. The reported tilt angles were smaller upon insertion of PFOS ( approximately 24 degrees ) than in the presence of PFOA ( approximately 30 degrees ). Overall, the results show that the incorporation of the perfluorinated acids has an effect on the bilayer similar to that of cholesterol by increasing the membrane fluidity and thickness due to a decrease in the tilt angle of the acyl chains.

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Interaction of xenobiotics with mercury-adsorbed phospholipid monolayers

Cited by 5 publications

References 8 publications

Interpreting Ion Fluxes to Channel Arrays in Monolayers

Interpreting Ion Fluxes to Channel Arrays in Monolayers

Organic Xenobiotic Metabolism in Marine Invertebrates

Polarization Modulation Infrared Reflection−Absorption Spectroscopy Studies of the Influence of Perfluorinated Compounds on the Properties of a Model Biological Membrane

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