Interactions among human T cell subsets

Thomas, Y; Sosman, Jeffrey A.; Irigoyen, Oscar H.; Rogozinski, Linda; Friedman, Steve; Chess, Leonard

doi:10.1016/0192-0561(81)90013-8

Cited by 23 publications

(15 citation statements)

References 23 publications

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“…In addition, we have previously reported that at the higher cell numbers required for the induction of specific antibody production by antigen, PWM-induced responses were generally absent. This latter observation is presumably explained by the finding, demonstrated here in an antigen-specific system and reported by others in polyclonal systems (1,5,6), that excessive numbers of T cells suppress a PWMdriven response.…”

Section: Discussionmentioning

confidence: 63%

“…The earliest in vitro observations on this phenomenon suggested that the suppression was solely at the level of the B cell with T cell help relatively radioresistant (23). However, subsequent studies in the murine system examining the nature of T cell help at limiting num- degree of radiosensitivity to the T cell help that could only be appreciated at limiting numbers of T cells (6). The previous studies on antigen-induced T cell help in the human system have been "conflicting," with some investigators noting radiation sensitivity and others noting a radiation resistance to antigen-induced T cell help (10,25).…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, there have been conflicting data among sev-. eral of these systems (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Of particular note has been the divergence of results with regard to the radiosensitivity of T cell help and the relative contributions of allogeneic effects in the mitogen-induced vs. antigen-induced human B cell systems.…”

Section: Introductionmentioning

confidence: 99%

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Antigen-induced human T cell help. Precursor frequency, radiation sensitivity, and allogeneic effects.

Lane¹,

Whalen²,

Fauci³

1983

J. Clin. Invest.

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A B S T R A C T We have recently noted marked differences between the in vitro responses of human B lymphocytes to stimulation with soluble antigens vs. stimulation with mitogens. In the present study, these differences were analyzed in terms of the precursor frequencies for the T cells and B cells involved and in terms of the radiation sensitivity of the T cells providing help in the two systems. Marked differences were found between antigen-induced and mitogen-induced systems with regard to T cell precursor frequencies and radiation sensitivity. In contrast, the precursor frequencies for the B cells involved in the two systems were approximately the same. In addition, having developed a system for the study of human antigen-specific B cell responses, we were interested in delineating the nature of the allogeneic effects that might be operative in this system. Marked allogeneic effects, both positive and negative, were noted in this system and will need to be taken into account in any studies that try to address the question of the genetic restriction, if any, that exists in human antigen-specific T cell-B cell collaboration.Appreciation of the marked differences between the antigen-specific and mitogen-induced activation and immunoregulation of human B cell responses will be of importance in understanding the relationship between specificity and nonspecificity of antibody production in normal and disease states. INTRODUCTIONT cell regulation of B cell reactivity is an area of major interest and importance in both animal and human systems. With the recent availability of several systems Address all correspondence to Dr. H. Clifford Lane. Received for publication 20 January 1983 and in revised form 30 March 1983. to study human B cell function in vitro, considerable interest has centered around the precise nature of the T cell regulation of human B cell responses in both polyclonal and antigen-specific assay systems. Unfortunately, there have been conflicting data among sev-. eral of these systems (1-10). Of particular note has been the divergence of results with regard to the radiosensitivity of T cell help and the relative contributions of allogeneic effects in the mitogen-induced vs. antigen-induced human B cell systems. Disagreement with regard to this latter point has led to difficulty in delineating the precise nature of genetic restrictions in T cell-B cell collaboration in antigen-specific responses as well in appreciating the scope of allogeneic effects in co-culture experiments now commonly used in the study of patients with immunemediated or immunodeficiency diseases.Recently, in vitro systems have been developed for the study of antigen-induced, antigen-specific antibody production and secretion into culture supernatants by human B cells (11)(12)(13)(14). The use of enzymelinked immunosorbent assays (ELISA)l has proven to be extremely useful in these studies because of their simplicity, sensitivity, and specificity. These advances have allowed for a more precise study of the mechanisms involved in...

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antigen-induced human T cell help. Precursor frequency, radiation sensitivity, and allogeneic effects.

Lane¹,

Whalen²,

Fauci³

1983

J. Clin. Invest.

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“…Moreover, irradiation of the ATL cells with the view of diminishing suppressor activity failed to enhance immunoglobulin production. In this regard, a number of laboratories has shown that the suppressor activity of cells is relatively radiosensitive, whereas helper activity seems to be relatively radioresistant (7,9). While no effect was seen with ATL cells, when normal T4 cells were irradiated, the effect of suboptimal numbers of irradiated T4 cells in providing help was less than the effect of unirradiated T4 cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Perhaps of greatest interest was the demonstration that communicative interactions occurred both between and within the major populations of cells in the generation of specific effector functions. For example, interactions between a subpopulation of T4 and T8 cells are required to induce suppression of Ig production in antigen specific, pokeweed mitogen (PWM)' driven and autologous mixed leukocyte reaction systems (7)(8)(9)(10). The human immunoregulatory circuit is clearly composed of discrete subsets of still poorly defined cells that interact to maintain homeostasis (10, 11).…”

Section: Introductionmentioning

confidence: 99%

Functional and phenotypic studies of Japanese adult T cell leukemia cells.

Morimoto¹,

Matsuyama²,

Oshige³

et al. 1985

J. Clin. Invest.

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The cell surface marker profile and functional analysis of peripheral blood lymphocytes from 11 Japanese adult T cell leukemia patients were studied.The phenotypic analysis of Japanese adult T cell leukemia (ATL) cells by a series of 13 monoclonal antibodies showed that all ATL cells are anti-T4 reactive but some differ in their expression of T3, Tl1, and T12 antigens. Thus, considerable phenotypic heterogeneity exists in these populations of leukemia cells. When analyzed in functional assays, ATL cells were suppressive when added to a pokeweed mitogen-(PWM) driven Ig synthesis system. However, the suppression mechanism seemed to be more complex than originally conceived. ATL cells examined in this study seem to function mainly as an inducer of suppressor cells, and as such, activate normal T8 precursors of suppressor cells rather than function as suppressor effector cells. In addition, no evidence was obtained to suggest that suppression of PWM-stimulated IgG synthesis was mediated by natural killer (NK) activity of ATL cells. Rather, ATL cells seem to be markedly deficient in NK activity.These studies suggest that the majority of ATL cells tested are representative of and seem to be the leukemic counterparts of the T4+ suppressor inducer subset.

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T-lymphocyte subpopulations in B-cell-derived non-hodgkin's lymphomas and hodgkin's disease

Knowles

Halper²,

Jakobiec³

1984

Cancer

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The authors used E‐rosette formation and OKT3 reactivity to determine the percent of T‐cells in lymph nodes involved by B‐cell non‐Hodgkin's lymphomas (B‐NHL) and by Hodgkin's disease (HD). The percent of helper and suppressor/cytotoxic T‐cells was determined by reactivity with OKT4 and OKT8, respectively. T‐cells were also analyzed for two signs of activation: acquisition of Ia antigens and loss of acid a‐naphthyl acetate esterase (ANAE) activity. The results were compared with those of lymph nodes exhibiting benign lymphoid hyperplasia (BLH). The percentage of T‐cells ranged from 50% to 82%, mean 63 ± 13%, in 25 cases of BLH, and from 6% to 62%, mean 23 ± 11%, in 51 cases of B‐NHL. The OKT4/T8 ratio was 1.0 to 6.2, mean 3.4 ± 2.2, in the cases of BLH, and 0.5 to 5.1, mean 2.4 ± 1.3, in the cases of B‐NHL. There was no obvious or significant correlation between the percent of T‐cells or the OKT4/T8 ratio and the surface immunoglobulin isotype expressed by the neoplastic B‐cells, the morphologic category of B‐NHL, or the clinical stage of disease. Activated T‐cells were ≦3% in the cases of BLH and B‐NHL. Fifteen lymph nodes involved by HD contained 44% to 96%, mean 74%, E+ (T) cells. Five of these 15 cases contained a significant number of E−OKT3+ cells suggesting that E‐rosette formation is not always a reliable T‐cell marker in HD. Three other cases contained a large number of E+OKT3− cells. The OKT4/T8 ratio ranged from 0.4 to 21.7, mean 6.7 ± 5.3, in these cases, representing the most significant T‐cell subset imbalances in this series. Large numbers of Ia+E+ and/or E+ ANAE− cells, presumably activated T‐cells, were present in 7 of these 15 cases of HD. These studies demonstrate the wide variation in the percent of T‐cells and in the T‐cell subset distribution in lymph nodes exhibiting benign lymphoid hyperplasia and in lymph nodes involved by B‐cell‐derived non‐Hodgkin's lymphomas and Hodgkin's disease.

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Interactions among human T cell subsets

Cited by 23 publications

References 23 publications

Antigen-induced human T cell help. Precursor frequency, radiation sensitivity, and allogeneic effects.

Antigen-induced human T cell help. Precursor frequency, radiation sensitivity, and allogeneic effects.

Functional and phenotypic studies of Japanese adult T cell leukemia cells.

T-lymphocyte subpopulations in B-cell-derived non-hodgkin's lymphomas and hodgkin's disease

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