Interactions between cholesterol and lecithins in monolayers at the air-water interface

Probing a Membrane Matrix Regulating Hormone Action

“…However, the shortening of molecules (and broadening of the cavity) should be relatively the same for all alkane derivatives. Tinoco and McIntosh's (22) data for the same lecithin we have pictured here indicates the maximally packed cross sectional area of this molecule in a monolayer is about 60 A2, more than would be predicted from either the linear or staggered molecular model. In reality, some more random configuration subject to thermal agitation must exist, especially well within the monolayer, while those parts of the chain nearest the hydrophilic surface and under the greatest surface pressures would be expected to assume a more uniform, regular array.…”

“…Cholesterol is about 21 A long and, because of its cyclic structure, could not undergo configuration chain shortening except for its tail. (22) have recently shown that the same phosphatidyl choline we have used for our illustration here, namely the 1-palmitoyl-2-linoleoyl compound, complexes with cholesterol. So did 1-stearoyl-2-linoleoyl, but its inverse l-linoleoyl-2-stearoyl (the only one tested) did not.…”

Condensing Effect of Cholesterol: A Physical Model

“…By assuming an area of 20 . AZ for the stearoyl moiety and 40 .AZ for the unsaturated chain (14), a value for AHC of 60 A2/molecule is determined, close to the area per molecule occupied by this lipid under high surface pressure (7). A s shown in Fig.…”

“…Of particular interest are mixed monolayers containing cholesterol and a phospholipid (1)(2)(3)(4)(5)(6)(7)(8). In most cases, the behavior of these mixed systems has been analyzed by plotting the mean area per molecule as a function of monolayer composition a t constant surface pressure.…”

Physical effects of 3-phenylindole on ion transport across bilayer lipid membranes

“…The condensing effect of cholesterol on phospholipid monolayers has been verified many times (van Deenan et !l., 1962;Standish and Pethica, 1967;Shah and Schulman, 1967;Demel and Joos, 196B;Tinoco and McIntosh, 1970;Cadenhead et !l., 1976).…”