Interactions between endogenous gamma interferon and tumor necrosis factor in host resistance against primary and secondary Listeria monocytogenes infections

Nakane, Akio; Minagawa, Tomonori; Kohanawa, Masashi; Chen, Y; Sato, Haruko; Moriyama, Masaya; Tsuruoka, Nobuo

doi:10.1128/iai.57.11.3331-3337.1989

Cited by 124 publications

(88 citation statements)

References 16 publications

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“…In our experiments, the production of endogenous IFN-3, in CNS tissue was observed from Day 5 to Day Suzuki et al, 1988;Leist et al, 1989;Nakane et al, 1989;Smith et al, 1991). There are only a few reports on the kinetics of endogenous IFN-7 production during infections.…”

Section: Discussionmentioning

confidence: 47%

“…Rat IgG~ (mAb) against the purified preparation of mouse natural IFN-7, which had been prepared from the ascites fluid in Pristane-primed CD-1 nude mice injected with hybridoma R4-6A2 (Nakane et al, 1989), was purified by (NH4)2SO 4 precipitation. The characteristics of the mAb were described previously (Spintalny and Havell, 1984).…”

Section: Antibodiesmentioning

confidence: 99%

“…IFN-y assay of the tissue homogenate was carried out by a double-sandwich enzyme-linked immunosorbent assay (ELISA) as previously reported (Nakane et al, 1990). Rat anti-mouse IFN-y mAb (R4-6A2) purified by DEAE Affi-Gel Blue column chromatography (Nakane et al, 1989) was used as a capture antibody. Rabbit anti-mouse IFN-y serum was obtained from a rabbit hyperimmunized with purified recombinant mouse IFN-T as described previously (Nakane et al, 1989).…”

Section: Ifn-t Assaymentioning

confidence: 99%

“…Rat anti-mouse IFN-y mAb (R4-6A2) purified by DEAE Affi-Gel Blue column chromatography (Nakane et al, 1989) was used as a capture antibody. Rabbit anti-mouse IFN-y serum was obtained from a rabbit hyperimmunized with purified recombinant mouse IFN-T as described previously (Nakane et al, 1989). All ELISAs were run with recombinant mouse IFN-T that was produced and purified by Genentech, Inc. (San Francisco, CA).…”

Section: Ifn-t Assaymentioning

confidence: 99%

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Endogenous gamma interferon produced in central nervous system by systemic infection infection with Theiler's virus in mice

Kohanawa¹,

Nakane²,

Minagawa³

1993

Journal of Neuroimmunology

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Theiler's virus GD VII strain causes acute encephalomyelitis by intracerebral inoculation. We established acute encephalomyelitis in mice by the intravenous (i.v.) inoculation of Theiler's virus GD VII strain. Replication of Theiler's virus injected i.v. could be observed in both the brain and spinal cord of mice, and interferon (IFN)-gamma could be detected in the extracts of brain and spinal cord in parallel with viral replication. Furthermore, by the injection of anti-IFN-gamma monoclonal antibody (mAb) on Day 1 post-infection (p.i.), mortality and virus titres in the spinal cord increased compared with the control mice treated with normal rat globulin. The histological exacerbation of inflammation was observed in spinal cord of anti-IFN-gamma mAb-treated mice. These results indicate that endogenous IFN-gamma, produced locally in the brain and spinal cord of mice through both antiviral action and anti-inflammatory action of IFN-gamma in central nervous system, plays an important role in Theiler's virus infection.

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Section: Discussionmentioning

confidence: 47%

Section: Antibodiesmentioning

confidence: 99%

Section: Ifn-t Assaymentioning

confidence: 99%

Section: Ifn-t Assaymentioning

confidence: 99%

See 2 more Smart Citations

Endogenous gamma interferon produced in central nervous system by systemic infection infection with Theiler's virus in mice

Kohanawa¹,

Nakane²,

Minagawa³

1993

Journal of Neuroimmunology

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“…The injected dose of the mAb was enough to a¡ect antilisterial resistance in mice (Fig. 3A) [38]. Therefore, it is assumed that endogenous IFN-Q production might not be enough to contribute to antilisterial resistance in aly/aly mice.…”

Section: Discussionmentioning

confidence: 98%

Dysregulation of interleukin-10 and interleukin-12 are involved in the reduced host resistance to Listeria monocytogenes infection in alymphoplastic aly mutant mice

Hasegawa

2002

FEMS Immunology and Medical Microbiology

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The aly is a unique spontaneous autosomal recessive mutation in mice that causes a systemic defect of lymph nodes and Peyer's patches and disorganized splenic and thymic structures with immunodeficiency. Our previous study demonstrated that resistance to Listeria monocytogenes infection and interferon-gamma (IFN-gamma) production are attenuated in the mutant mice. In this study, we investigated the mechanism of decrease in antilisterial resistance and IFN-gamma production in aly mice. Interleukin (IL)-12 production in response to heat-killed L. monocytogenes (HK-LM) was decreased but IL-10 production was increased in aly/aly macrophage cultures, compared with those in aly/+ macrophages. Nonadherent cells and macrophages obtained from the spleens of naive aly/+ mice and aly/aly mice were reconstituted and stimulated with HK-LM. IFN-gamma production was markedly decreased when macrophages derived from aly/aly mice were used. IFN-gamma production in aly/aly spleen cell cultures was recovered in the presence of anti-IL-10 monoclonal antibody (mAb) or recombinant IL-12. When aly/+ mice and aly/aly mice were injected with mAb against IL-10 or IL-12 p40, antilisterial resistance was inhibited by injection of anti-IL-12 p40 mAb, while anti-IL-10 mAb treatment augmented the resistance. Administration of anti-IFN-gamma mAb attenuated antilisterial resistance in aly/+ mice but not in aly/aly mice. The present results suggest that downregulation of IL-12 and upregulation of IL-10 in macrophages might be involved in the decrease in antilisterial resistance and IFN-gamma production in aly/aly mice in addition to the structural defect in lymphoid organs. Moreover, the results predict that an IL-12-dependent and IFN-gamma-independent mechanism may be also involved in the decrease in antilisterial resistance in aly/aly mice.

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Reduced antilisterial activity of TNF-deficient bone marrow-derived macrophages is due to impaired superoxide production

Müller

Althaus²,

Fröhlich

et al. 1999

Eur. J. Immunol.

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Mice deficient for TNF ligand and receptor type 1 have demonstrated the importance of TNF in the host defense against Listeria monocytogenes. To investigate the particular deficiency of macrophages derived from TNF/lymphotoxin (LT)‐α− / − mice in antilisterial growth control, bone marrow‐derived macrophages (BMDM) were used for in vitro infection experiments. After the combined treatment with IFN‐γ and lipopolysaccharide (LPS), production of NO by wild‐type (wt) and TNF/LT‐α− / − BMDM was induced to comparable levels, but only wt BMDM controlled L. monocytogenes growth efficiently. Nevertheless, inhibition of NO production led to a remarkable loss of antilisterial activity. This suggests that presence of NO is necessary but not sufficient for L. monocytogenes killing and that elimination of L. monocytogenes requires additional effector molecules. The LPS‐inducible superoxide production of TNF/LT‐α− / − BMDM was impaired. Accordingly both scavenging of superoxide and peroxynitrite led to reduced L. monocytogenes killing by wt BMDM. In addition, peroxynitrite was able to kill L. monocytogenes in vitro. Together these findings suggest that the defective host defense of TNF/LT‐α‐deficient mice against L. monocytogenes partially stems from reduced superoxide production of macrophages due to the absence of TNF and imply a function for peroxynitrite, the reaction product of NO and superoxide, in the intracellular killing of L. monocytogenes.

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Interactions between endogenous gamma interferon and tumor necrosis factor in host resistance against primary and secondary Listeria monocytogenes infections

Cited by 124 publications

References 16 publications

Endogenous gamma interferon produced in central nervous system by systemic infection infection with Theiler's virus in mice

Endogenous gamma interferon produced in central nervous system by systemic infection infection with Theiler's virus in mice

Dysregulation of interleukin-10 and interleukin-12 are involved in the reduced host resistance to Listeria monocytogenes infection in alymphoplastic aly mutant mice

Reduced antilisterial activity of TNF-deficient bone marrow-derived macrophages is due to impaired superoxide production

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