Interactions between human neutrophils and mucin-coated surfaces

Abstract

Recently, we showed microscopically that bovine (BSM), porcine (PGM) and human (MG1) mucin coatings could suppress the adhesion of neutrophils to a polyethylene terephthalate-based model biomaterial (Thermanox). Here, using the release of reactive oxygen species (ROS) as a marker of material-induced neutrophil activation, the strong surface-passivating effects of these mucin coatings were corroborated. Under optimal adsorption conditions, all mucin species performed equally well, thus indicating a high degree of functional homology between the mucins. Cell adhesion and morphology correlated well with the release of ROS. Quartz crystal microbalance (QCM-D) analysis linked low neutrophil activation to efficient mucin surface-shielding. Interestingly, the shielding power appeared equal for thick expanded and thin compact mucin coatings. Combined mucin-serum coatings were found to be highly surface-passivating. Particularly, since our data suggested partly synergistic mucin-serum action, we highlight the possibility that pre-adsorbed mucins could provide favorable support for adsorbing host components.

Potential use of mucins as biomaterial coatings. II. Mucin coatings affect the conformation and neutrophil‐activating properties of adsorbed host proteins—Toward a mucosal mimic

“…While the BSM and PGM mucins were prepared to represent highly purified mucin species, the MG1 mucin was prepared to retain naturally associated biomolecules and thus represent a complex and presumed functional mucin. In two subsequent investigations, we have studied the activation of human neutrophils, which are important cellular mediators of material‐induced inflammation, upon contact with a polyethylene terephthalate‐based model biomaterial (Thermanox) that was coated with these mucins 31, 32. Our results showed all three mucins to be capable of reducing the neutrophil adhesion and activation.…”

“…Our results showed all three mucins to be capable of reducing the neutrophil adhesion and activation. Specifically, from a combination of microscopic analyses of the neutrophil adherence and morphology with fundamental surface analyses we showed that the overall performance of the formed mucin coatings relied on the ability of the mucin layer to shield the underlying surface 32. In case of the complex MG1 coating, the surface‐shielding effect was believed to depend on associated components in the mucin structure 31, 32.…”

Real-time analysis of dual-display phage immobilization and autoantibody screening using quartz crystal microbalance with dissipation monitoring

“…19 Specifically, the slope of the line between the initial and final point of adsorption, k D-f = ∆D/∆f scales differently with different adlayer conformations. This means that rigid and compact layers yield low k D-f values, whereas bulky and diffuse layers yield high k D−f values.…”

“…This means that rigid and compact layers yield low k D-f values, whereas bulky and diffuse layers yield high k D−f values. 19 Figure 2A shows the different slopes upon STV and SBP displaying phage adsorption. As it can be observed, adsorption of STV yields a k D-f =-0.01, while adsorption of SBP displaying phage k D-f =-2.09.…”

Polymer Thin Films for Biomedical Applications

“…The sulfated brushes also reduced the production of complement factor products C3a, C4a, and C5a, in comparison to the control surfaces. Other polysaccharide -based glycocalyx -mimicking polymer coatings that reduce platelet adhesion and improve blood compatibility have also been described [68,69] .…”