Potential use of mucins as biomaterial coatings. II. Mucin coatings affect the conformation and neutrophil‐activating properties of adsorbed host proteins—Toward a mucosal mimic

Abstract

In continuation of our recent fractionation and characterization study on mucins of bovine salivary (BSM), porcine gastric (PGM), and human salivary (MG1) origin, this study evaluates the effect of mucin precoating on the conformation and neutrophil-activating properties of host proteins adsorbed to a polyethylene terephthalate-based model biomaterial. Microscopy combined with assays for the neutrophil releases of reactive oxygen species and human neutrophil lipocalin showed that mucin precoating greatly reduced the strong immune-response normally induced by adsorbed immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), respectively. A similar finding was made for the proinflammatory fibrinogen. Although the total uptakes of these proteins depended on the mucin surface concentration, a detailed composite analysis suggested the fraction of surface-exposed protein to be a stronger determinant of coating performance. The unexpectedly low neutrophil activation showed by composites containing near-monolayer concentrations of exposed IgG and sIgA, respectively, suggested that these act synergistically with mucin on the surface. In support of this hypothesis, quartz crystal microbalance with dissipation monitoring measurements revealed that a preadsorbed BSM layer stabilizes IgG through complexation on a polymeric model surface. Our findings link well to the complex in vivo situation and suggest that functional mucosal mimics can be created in situ for improved biomaterials performance.

Influence of impurities and contact scale on the lubricating properties of bovine submaxillary mucin (BSM) films on a hydrophobic surface

“…Previous studies have shown that various other biophysical properties than lubrication of commercial mucins, such as viscoelasticity and surface forces, are also greatly changed after chromatographic purification, and the differences were mainly attributed to the presence of BSA in as-received BSM samples [18,20,22,39]. Thus, tribological properties of BSA (1 mg mL −1 in PBS), as well as a mixture of ae-BSM/BSA (20/1 v/v) to simulate ar-BSM [20] have also been characterized.…”

Chitosan adsorption on hydroxyapatite and its role in preventing acid erosion

“…Co. USA) and a conductivity meter (Mettler-Toledo, Switzerland), respectively. For QCM-D studies, we used an artificial saliva solution, which contains only two proteins (mucin and albumin) [56,57], because the composition of this layer is less complex than that of the many component salivary film [58]. First, the porcine mucin and the salts solutions were made separately.…”

Isolation, Biochemical Characterization and Anti-adhesion Property of Mucin from the Blue Blubber Jellyfish (<i>Catostylus mosaicus</i>)

“…Mucins in general may have potential for wider commercial applications due to their moisture retention, high viscosity and lubrication abilities. For example, they are considered to be potent protective coating substances for use in the field of biomaterials (Chen et al, 2004;Sandberg et al, 2009) due to their resistance to proteolytic degradation and ability to aggregate and form lubricating gels. They are also generally biocompatible and non-toxic.…”