Chitosan adsorption on hydroxyapatite and its role in preventing acid erosion

Lee, Hyun Su; Tsai, Shannon; Kuo, Chung‐Hao; Bassani, Alice W.; Pepe-Mooney, Brian; Miksa, Davide; Masters, James G.; Sullivan, Richard; Composto, Russell J.

doi:10.1016/j.jcis.2012.06.074

Cited by 57 publications

(48 citation statements)

References 65 publications

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“…The interaction of chitosan with such negatively charged surfaces is complex. It is assumed that the molecule forms multilayers stable at acidic pH [Claesson and Ninham, 1992], resulting in a lower susceptibility of the underlying substrate to erosive demineralisation [Lee et al, 2012]. It can also bind to mucin, occurring in the pellicle structure, with the possibility to form multilayers with the mucin molecule [Dedinaite et al, 2005;Svensson et al, 2006].…”

Section: Discussionmentioning

confidence: 99%

Randomised in situ Study on the Efficacy of a Tin/Chitosan Toothpaste on Erosive-Abrasive Enamel Loss

Schlueter

Klimek²,

Ganß³

2013

Caries Res

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Tin is a notable anti-erosive agent, and the biopolymer chitosan has also shown demineralisation-inhibiting properties. Therefore, the anti-erosive/anti-abrasive efficacy of the combination of both compounds was tested under in situ conditions. Twenty-seven volunteers were included in a randomised, double-blind, three-cell crossover in situ trial. Enamel specimens were recessed on the buccal aspects of mandibular appliances, extraorally demineralised (6 × 2 min/day) and intraorally treated with toothpaste slurries (2 × 2 min/day). Within the slurry treatment time, one-half of the specimens received additional intraoral brushing (5 s, 2.5 N). The tested toothpastes included a placebo toothpaste, an experimental NaF toothpaste (1,400 ppm F^-) and an experimental F/Sn/chitosan toothpaste (1,400 ppm F^-, 3,500 ppm Sn²⁺, 0.5% chitosan). The percentage reduction of tissue loss (slurry exposure/slurry exposure + brushing) compared to placebo was 19.0 ± 47.3/21.3 ± 22.4 after use of NaF and 52.5 ± 30.9/50.2 ± 34.3 after use of F/Sn/chitosan. F/Sn/chitosan was significantly more effective than NaF (p ≤ 0.001) and showed good efficacy against erosive and erosive-abrasive tissue loss. This study suggests that the F/Sn/chitosan toothpaste could provide good protection for patients who frequently consume acidic foodstuffs.

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Section: Discussionmentioning

confidence: 99%

Randomised in situ Study on the Efficacy of a Tin/Chitosan Toothpaste on Erosive-Abrasive Enamel Loss

Schlueter

Klimek²,

Ganß³

2013

Caries Res

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“…Consequently, an important challenge consists in synthesizing a chitosan-based dental implant resistant at low pH values. Previous work has already highlighted acidic resistance of chitosan film on hydroxyapatite substrate [30]. Our group has also recently proved the acidic pH resistance of grafted chitosan using TESPSA as coupling agent [24,31].…”

Section: Introductionmentioning

confidence: 93%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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“…Chitosan is a natural polysaccharide derived from chitin, mainly from shells of shrimps and other crustaceous [Synowiecki and Al-Khateeb, 2003]. It is a biopolymer, which is positively charged [Tharanathan and Kittur, 2003] and strongly binds with its amino groups [Lee et al, 2012] to surfaces with negative zeta-potential [Young et al, 1997], such as dental hard tissue. It is more than likely that the reactive molecule chitosan reacted with the enamel surface in all the chitosan-based groups of the present study and led to a coverage of, at least, a thin, ubiquitous chitosan layer [Lee et al, 2012].…”

Section: Discussionmentioning

confidence: 99%

“…It is a biopolymer, which is positively charged [Tharanathan and Kittur, 2003] and strongly binds with its amino groups [Lee et al, 2012] to surfaces with negative zeta-potential [Young et al, 1997], such as dental hard tissue. It is more than likely that the reactive molecule chitosan reacted with the enamel surface in all the chitosan-based groups of the present study and led to a coverage of, at least, a thin, ubiquitous chitosan layer [Lee et al, 2012]. Such chitosan-based layers have a thickness of few nanometres [Tiraferri et al, 2014] and are, therefore, not visible under the SEM with the magnifications used in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…One can, therefore, only speculate about what really happened on the surface. However, a comprehensive study investigating the reaction between hydroxyapatite, coated with artificial saliva, and chitosan could give indications [Lee et al, 2012]. It revealed that chitosan forms layers on the surface, which are differently structured depending on the environment.…”

Section: Discussionmentioning

confidence: 99%

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In vitro Efficacy of Experimental Chitosan-Containing Solutions as Anti-Erosive Agents in Enamel

Pini

Lima²,

Lovadino³

et al. 2016

Caries Res

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The present study evaluated the effect of chitosans with different viscosities, dissolved in an AmF/SnCl₂ solution, against erosion or erosion/abrasion. A total of 192 specimens were assigned to 2 × 6 groups (n = 16 specimens each): negative control, 4 chitosan solutions (groups Ch50, Ch500, Ch1000, and Ch2000, with viscosity of 50, 500, 1,000, or 2,000 mPas, respectively, 0.5% chitosan, 500 ppm F^-, 800 ppm Sn²⁺, pH 4.4), and positive control (500 ppm F^-, 800 ppm Sn²⁺, pH 4.3). One half of the groups was demineralized (experiment 1, E1; 10 days, 6 × 2 min/day, 0.5% citric acid, pH 2.8) and exposed to solutions (2 × 2 min/day); the other half was additionally brushed (15 s, 200 g) with non-fluoridated toothpaste before solution immersion (experiment 2, E2). Treatment effects were investigated by profilometry, energy-dispersive X-ray spectroscopy and scanning electron microscopy (SEM). In E1, all the chitosan-containing solutions reduced enamel loss by 77-80%, to the same extent as the positive control, except for Ch2000 (p ≤ 0.05), which completely inhibited tissue loss by the formation of precipitates. In E2, Ch50 and Ch500 showed best performance, with approximately 60% reduction of tissue loss compared to the negative control group (p ≤ 0.05 compared to other groups). SEM analysis showed differences between negative control and the other groups but only minor differences amongst the groups treated with active agents. In both E1 and E2, treatment with active agents resulted in surface enrichment of carbon and tin compared to negative control (p ≤ 0.001); brushing removed parts of carbon and tin (p ≤ 0.001). Chitosan shows different properties under erosive and erosive/abrasive conditions. Under erosive conditions high viscosity might be helpful, whereas lower viscosity seems to be more effective in cases of chemo-mechanical challenges.

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Chitosan adsorption on hydroxyapatite and its role in preventing acid erosion

Cited by 57 publications

References 65 publications

Randomised in situ Study on the Efficacy of a Tin/Chitosan Toothpaste on Erosive-Abrasive Enamel Loss

Randomised in situ Study on the Efficacy of a Tin/Chitosan Toothpaste on Erosive-Abrasive Enamel Loss

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

In vitro Efficacy of Experimental Chitosan-Containing Solutions as Anti-Erosive Agents in Enamel

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