Interactions between hydrogen sulphide and nitric oxide regulate two soybean citrate transporters during the alleviation of aluminium toxicity

Abstract

Hydrogen sulphide (H 2 S) is emerging as an important signalling molecule involved in plant resistance to various stresses. However, the underlying mechanism of H 2 S in aluminium (Al) resistance and the crosstalk between H 2 S and nitric oxide (NO) in Al stress signalling remain elusive. Citrate secretion is a wide-spread strategy for plants against Al toxicity. Here, two citrate transporter genes, GmMATE13 and GmMATE47, were identified and characterized in soybean. Functional analysis in Xenopus oocytes and transgenic Arabidopsis showed that GmMATE13 and GmMATE47 mediated citrate exudation and enhanced Al resistance. Al treatment triggered H 2 S generation and citrate exudation in soybean roots. Pretreatment with an H 2 S donor significantly elevated Al-induced citrate exudation, reduced Al accumulation in root tips, and alleviated Al-induced inhibition of root elongation, whereas application of an H 2 S scavenger elicited the opposite effect. Furthermore, H 2 S and NO mediated Al-induced GmMATE expression and plasma membrane (PM) H + -ATPase activity and expression. Further investigation showed that NO induced H 2 S production by regulating the key enzymes involved in biosynthesis and degradation of H 2 S. These findings indicate that H 2 S acts downstream of NO in mediating Al-induced citrate secretion through the upregulation of PM H + -ATPase-coupled citrate transporter cotransport systems, thereby conferring plant resistance to Al toxicity.

Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage

“…However, pretreatment with the NO donor sodium nitroprusside (SNP) enhances the activity of l -CD, which, in turn, induces accumulation of endogenous H 2 S in maize seedlings [49]. NO induces H 2 S production by regulating the key enzymes involved in biosynthesis and degradation of H 2 S in soybean [50]. Nitrate reductase-dependent NO production is involved in H 2 S-induced nitrate stress tolerance in tomato seedling [51].…”

OsProT1 and OsProT3 Function to Mediate Proline- and γ-aminobutyric acid-specific Transport in Yeast and are Differentially Expressed in Rice (Oryza sativa L.)

“…Transformed protoplasts were incubated in the dark at 22 °C overnight, and eGFP images were subsequently obtained using confocal microscopy (Olympus-FV1000) (Peng et al 2017). The fluorescent dye Dil was used at a concentration of 10 μM to indicate the plasma membrane (Wang et al 2019).…”

Plant Proton Pumps and Cytosolic pH-Homeostasis

“…This might be due to H 2 S-mediated release of inhibitory disulfide bridges ( Kabala et al, 2019 ). Similarly, PM-ATPase activity was reported to be enhanced by H 2 S in soy bean roots ( Wang et al, 2019 ).…”