Interactions between leaf pubescence genes in bread wheat as assessed by high throughput phenotyping

Дорошков, А. В.; Afonnikov, D. A.; Dobrovolskaya, O. B.; Пшеничникова, Т. А.

doi:10.1007/s10681-015-1520-2

Cited by 16 publications

(10 citation statements)

References 28 publications

(39 reference statements)

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“…The hexaploid Triticum aestivum L. cultivars Saratovskaya 29 (S29, a resistant cultivar) and Yanetzkis Probat (YP, a susceptible cultivar) (Doroshkov, Pshenichnikova & Afonnikov, 2011;Doroshkov et al, 2016) were subjected to transcriptome analyses upon the standard, water deficiency (WD) and low-temperature conditions. Germinated seeds for both cultivars were planted in plastic containers with drainage (PET, size 56 × 39 × 28 cm) filled with expanded clay balls.…”

Section: Plant Material Stress Treatments and Samplingmentioning

confidence: 99%

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Konstantinov

Зубаирова

Ermakov

et al. 2021

PeerJ

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Bread wheat (Triticum aestivum L.) is one of the most important agricultural plants wearing abiotic stresses, such as water deficit and cold, that cause its productivity reduction. Since resistance to abiotic factors is a multigenic trait, therefore modern genome-wide approaches can help to involve various genetic material in breeding. One technique is full transcriptome analysis that reveals groups of stress response genes serving marker-assisted selection markers. Comparing transcriptome profiles of the same genetic material under several stresses is essential and makes the whole picture. Here, we addressed this by studying the transcriptomic response to water deficit and cold stress for two evolutionarily distant bread wheat varieties: stress-resistant cv. Saratovskaya 29 (S29) and stress-sensitive cv. Yanetzkis Probat (YP). For the first time, transcriptomes for these cultivars grown under abiotic stress conditions were obtained using Illumina based MACE technology. We identified groups of genes involved in response to cold and water deficiency stresses, including responses to each stress factor and both factors simultaneously that may be candidates for resistance genes. We discovered a core group of genes that have a similar pattern of stress-induced expression changes. The particular expression pattern was revealed not only for the studied varieties but also for the published transcriptomic data on cv. Jing 411 and cv. Fielder. Comparative transcriptome profiling of cv. S29 and cv. YP in response to water deficit and cold stress confirmed the hypothesis that stress-induced expression change is unequal within a homeologous gene group. As a rule, at least one changed significantly while the others had a relatively lower expression. Also, we found several SNPs distributed throughout the genomes of cv. S29 and cv. YP and distinguished the studied varieties from each other and the reference cv. Chinese Spring. Our results provide new data for genomics-assisted breeding of stress-tolerant wheat cultivars.

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Section: Plant Material Stress Treatments and Samplingmentioning

confidence: 99%

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Konstantinov

Зубаирова

Ermakov

et al. 2021

PeerJ

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“…In line i: S29 Hl2 aesp with gene introgression from Ae. speltoides, the density of trichomes on different sides of the leaf and under different conditions in creased 1.08-1.17 times, and the length of trichomes increased 1.6 times compared to the recipient (Doroshkov et al, 2016;Pshenichnikova et al, 2019). The lines are a convenient model for studying the role that pubescence genes play in wheat stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…The line i: S29 hl1, hl3 has a poor pubescence, while the leaves of the second line were densely pubescent. The origin, genetic characteristics and the quantitative charac teristics of the pubescence in leaves of the nearisogenic lines have been described in detail earlier (Doroshkov et al, 2016;Pshenichnikova et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…speltoides (Pshenichnikova et al, 2007). Hl1 and Hl3 affect to a greater extent on trichomes initiation and growth, while Hl2 regulates the length of trichomes (Doroshkov et al, 2016). Knowledge of the relationship of these genes with the physiological characteristics of drought tolerance and grain productivity is necessary for their including in the breeding process.…”

Section: Introductionmentioning

confidence: 99%

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Physiological responses to water deficiency in bread wheat (Triticum aestivum L.) lines with genetically different leaf pubescence

et al. 2020

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Studying the relationship between leaf pubescence and drought resistance is important for assessing Triticum aestivum L. genetic resources. The aim of the work was to assess resistance of common wheat genotypes with different composition and allelic state of genes that determine the leaf pubescence phenotype. We compared the drought resistance wheat variety Saratovskaya 29 (S29) with densely pubescent leaves, carrying the dominant alleles of the Hl1 and Hl3 genes, and two near isogenic lines, i: S29 hl1, hl3 and i: S29 Hl2aesp, with the introgression of the additional pubescence gene from diploid species Aegilops speltoides. Under controlled conditions of the climatic chamber, the photosynthetic pigments content, the activity of ascorbate-glutathione cycle enzymes and also the parameters of chlorophyll fluorescence used to assess the physiological state of the plants photosynthetic apparatus were studied in the leaves of S29 and the lines. Tolerance was evaluated using the comprehensive index D, calculated on the basis of the studied physiological characteristics. The recessive state of pubescence genes, as well as the introduction of the additional Hl2aesp gene, led to a 6-fold decrease in D. Under the water deficit influence, the fluorescence parameters profile changed in the lines, and the viability index decreased compared with S29. Under drought, the activity of ascorbate peroxidase, glutathione reductase and dehydroascorbate reductase in the line i: S29 hl1, hl3 decreased 1.9, 3.3 and 2.3 times, in the line i: S29 Hl2aesp it decreased 1.8, 3.6 and 1.8 times respectively, compared with S29. In a hydroponic greenhouse, line productivity was studied. Compared with S29, the thousand grains mass in the line i: S29 hl1, hl3 under water deficit was reduced. The productivity of the line i: S29 Hl2aesp was significantly reduced regardless of water supply conditions in comparison with S29. Presumably, the revealed effects are associated with violations of cross-regulatory interactions between the proteins of the trichome formation network and transcription factors that regulate plant growth and stress response.

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“…S29 possesses an increased tolerance to stress conditions and the cv. YP, on the contrary, is less tolerant (Doroshkov, Pshenichnikova & Afonnikov, 2011; Doroshkov et al, 2016). Therefore, this pair of genotypes was chosen to study stress-induced changes in the expression of the AOS genes in plant leaves.…”

Section: Methodsmentioning

confidence: 99%

Stress-induced changes in the expression of antioxidant system genes for rice (Oryza sativa L.) and bread wheat (Triticum aestivum L.)

Ermakov

Bobrovskikh

Зубаирова

et al. 2019

PeerJ

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BackgroundPlant cell metabolism inevitably forms reactive oxygen species (ROS), which can damage cells or lead to their death. The antioxidant system (AOS) evolved to eliminate a high concentration of ROS. For plants, this system consists of the seven classes of antioxidant enzymes and antioxidant compounds. Each enzymatic class contains a various number of genes which may vary from species to species. In such a multi-copy genetic system, the integration of evolutionary characteristics and expression data makes it possible to effectively predict promising breeding targets for the design of highly-yielding cultivars. In the plant cells, ROS production can increase as a result of abiotic stresses. Accordingly, AOS responds to stress by altering the expression of the genes of its components. Expression profiles of AOS enzymes, including their changes under stress, remains incomplete. A comprehensive study of the system behavior in response to stress for different species gives the key to identify the general mechanisms of AOS regulation. In this article, we studied stress-induced changes in the expression of AOS genes in photosynthetic tissues for rice and bread wheat.MethodsA meta-analysis of genome-wide transcriptome data on stress-induced changes in expression profiles of antioxidant genes using microarray and next generation sequencing (NGS) experiments from the GEO NCBI database for rice and bread wheat was carried out. Experimental study of expression changes in short (6 h) and prolonged (24 h) cold stress responses for selected AOS genes of bread wheat cultivars Saratovskaya29 and Yanetzkis Probat was conducted using qPCR.ResultsThe large-scale meta-transcriptome and complementary experimental analysis revealed a summary of fold changes in the AOS gene expression in response to cold and water deficiency for rice and bread wheat.

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Interactions between leaf pubescence genes in bread wheat as assessed by high throughput phenotyping

Cited by 16 publications

References 28 publications

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Physiological responses to water deficiency in bread wheat (Triticum aestivum L.) lines with genetically different leaf pubescence

Stress-induced changes in the expression of antioxidant system genes for rice (Oryza sativa L.) and bread wheat (Triticum aestivum L.)

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