Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring

Wang, Xiaoyan; Wang, Peipei; Song, Junlong; Jin, Yongcan; Rojas, Orlando J.

doi:10.1007/s10570-020-03070-4

Cited by 33 publications

(7 citation statements)

References 58 publications

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“…The interactions between PSS cellulase were monitored on an E4 module of quartz crystal microbalance with dissipation monitoring (QCM-D, Biolin Scienti c Co. Ltd, Gothenburg, Sweden) and a multi-parametric surface plasmon resonance (MP-SPR, BioNavis Co. Ltd., Tampere, Finland). The procedures for QCM and SPR sensors' cleaning, cellulase immobilization were described in detail elsewhere [33,55,56]. After cellulase immobilization and once the frequency and dissipation curves of QCM-D/SPR stabilized, the PSS solutions (with the concentration of 4.5 mg/mL) were loaded into the chambers until they reached another equilibrium state.…”

Section: Interactions Between Pss and Cellulase Monitored By Qcm-d And Mp-sprmentioning

confidence: 99%

Polystyrene Sulfonate is Effective for Enhancing Biomass Enzymatic Saccharification Under Green Liquor Pretreatment in Bioenergy Poplar

Liu

Wang

Tian

et al. 2021

Preprint

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Background: Water-soluble lignin (particularly lignosulfonate, LS) has been well documented for its significance on enzymatic saccharification of lignocellulose, though the promotion mechanism has not been fully understood. Much attention has been paid to natural lignin or its derivatives. The disadvantage of using natural lignin-based polymers as promoting agents lies in the difficulty in tailor-incorporating functional groups due to their complex 3D structures. To further improve our understanding on the promotion mechanism of water-soluble lignin in the bio-conversion of lignocellulose and to pursue better alternatives with different skeleton structures other than natural lignin, herein we reported a synthetic soluble linear aromatic polymer, sodium polystyrene sulfonate (PSS), to mimic LS for enhancing the efficiency of enzymatic saccharification. Results: The role of PSS played in enzymatic saccharification of pure cellulose and green liquor pretreated poplar (GL-P) was explored by analyzing substrate enzymatic digestibility (SED) under different addition dosages and various pH media, along with LS for comparison. At the cellulase loading of 13.3 FPU/g-glucan, the glucose yield of GL-P increased from 53% for the control to 81.5% with PSS addition of 0.1 g/g-substrate. It outperformed LS with the addition of 0.2 g/g-substrate by 6.3%. In the pH range from 4.5 to 6, PSS showed a positive effect on lignocellulose saccharification with the optimum pH at 4.8, where the most pronounced SED of GL-P was achieved. The underlying mechanism was unveiled by measuring zeta potential and using Quartz Crystal Microbalance (QCM) and Multi-parametric Surface Plasmon Resonance (MP-SPR). The results confirmed that the complexes of cellulase and PSS were conjugated and the negatively superchanged complexes reduced non-productive binding effectively along with the improved saccharification efficiency. The thickness of PSS required to block the binding sites of cellulase film was less than half of that of LS, and the PSS adlayer on cellulase film is also more hydrated and with a much lower shear modulus than LS adlayer. Conclusions: PSS as LS analogue is effective for enhancing the biomass enzymatic saccharification of GL-pretreated poplar. PSS exhibited a severer inhibition on the enzymatic saccharification of pure cellulose, whilst a more positive effect on bioconversion of lignocellulose (GL-P) than LS. In addition, a much lower dosage is required by PSS. The dynamic enzymatic hydrolysis indicated PSS could prolong the processive activity of cellulase. The valid data stemmed from QCM and SPR expressed that PSS bound to cellulases and the as-formed complexes reduced the nonproductive adsorption of cellulase onto substrate lignin more efficiently than LS due to its flexible skeleton and highly hydrated structure. Therefore, PSS is a promising alternative promoting agent for lignocellulose saccharification. From another perspective, the synthetic lignin mimics with controllable structures enable us to reach an in-depth understanding of the promotion mechanism of soluble lignins on enzymatic saccharification.

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Section: Interactions Between Pss and Cellulase Monitored By Qcm-d And Mp-sprmentioning

confidence: 99%

Polystyrene Sulfonate is Effective for Enhancing Biomass Enzymatic Saccharification Under Green Liquor Pretreatment in Bioenergy Poplar

Liu

Wang

Tian

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

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“…The van’t Hoff equation was used to analyze the standard entropy change (Δ S ) and enthalpy change (Δ H ) of BH ht and BH ae at different temperatures where the corresponding Δ H and Δ S were assessed by a plot of ln K a versus 1/ T , which yields a straight line with an intercept of Δ S / R and a slope of −Δ H / R . Figure shows the van’t Hoff plot for the determination of the thermodynamic parameters that are presented in Table .…”

Section: Results and Discussionmentioning

confidence: 99%

Kinetics and Thermodynamics of Hemicellulose Adsorption onto Nanofibril Cellulose Surfaces by QCM-D

et al. 2021

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The adsorption of hemicellulose derived from bagasse onto nanofibril cellulose has been studied in terms of kinetics and thermodynamics. In situ monitoring of bagasse hemicellulose with different molecular weights onto the nanofibril cellulose surfaces has been investigated using quartz crystal microbalance and dissipation. Then, the adsorption kinetics and thermodynamic properties were analyzed. Also, the sorption behavior and the adsorption layer properties were quantified in aqueous solutions. The maximum adsorption mass was 2.8314 mg/m2 at a concentration of 200 mg/L. Also, compared with that of the low-molecular-weight hemicellulose, the adsorption capacity of the high-molecular-weight hemicellulose was higher, and the adsorption rate changed faster and could reach an equilibrium in a shorter time. The intraparticle diffusion kinetic model represented the experimental data very well. Therefore, the kinetics of hemicellulose on the fiber adsorption was commonly described by a three-stage process: mass to transfer, diffusion, and equilibrium. The Gibbs energy change of the adsorption of hemicellulose was found to range from −20.04 to −49.75 kJ/mol at 25 °C. The entropy change was >0. It was found that the adsorption was spontaneous, and the adsorbed mass increased with the increase in temperature. This strengthened the conclusion that the adsorption process of the bagasse hemicellulose on the NFC was driven by the increase in entropy caused by the release of water molecules due to hydrophobic interaction or solvent reorganization.

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“…The interactions between PSS and cellulase were monitored on an E4 module of quartz crystal microbalance with dissipation monitoring (QCM-D, Biolin Scientific Co. Ltd, Gothenburg, Sweden) and a multi-parametric surface plasmon resonance (MP-SPR, BioNavis Co. Ltd., Tampere, Finland). The procedures for QCM and SPR sensors’ cleaning, cellulase immobilization were described in detail elsewhere [ 33 , 62 , 63 ]. After cellulase immobilization and once the frequency and dissipation curves of QCM-D/SPR stabilized, the PSS solutions (with the concentration of 4.5 mg/mL) were loaded into the chambers until they reached another equilibrium state.…”

Section: Methodsmentioning

confidence: 99%

Polystyrene sulfonate is effective for enhancing biomass enzymatic saccharification under green liquor pretreatment in bioenergy poplar

Liu

Wang

Tian

et al. 2022

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

Background Water-soluble lignin (particularly lignosulfonate, LS) has been well documented for its significance on enzymatic saccharification of lignocellulose, though the promotion mechanism has not been fully understood. Much attention has been paid to natural lignin or its derivatives. The disadvantage of using natural lignin-based polymers as promoting agents lies in the difficulty in tailor-incorporating functional groups due to their complex 3D structures. To further improve our understanding on the promotion mechanism of water-soluble lignin in the bioconversion of lignocellulose and to pursue better alternatives with different skeleton structures other than natural lignin, herein we reported a synthetic soluble linear aromatic polymer, sodium polystyrene sulfonate (PSS), to mimic LS for enhancing the efficiency of enzymatic saccharification. Results The role of PSS in enzymatic saccharification of pure cellulose and green liquor-pretreated poplar (GL-P) was explored by analyzing substrate enzymatic digestibility (SED) under different addition dosages and various pH media, along with LS for comparison. At the cellulase loading of 13.3 FPU/g-glucan, the glucose yield of GL-P increased from 53% for the control to 81.5% with PSS addition of 0.1 g/g-substrate. It outperformed LS with the addition of 0.2 g/g-substrate by 6.3%. In the pH range from 4.5 to 6, PSS showed a positive effect on lignocellulose saccharification with the optimum pH at 4.8, where the most pronounced SED of GL-P was achieved. The underlying mechanism was unveiled by measuring zeta potential and using Quartz Crystal Microbalance (QCM) and Multi-parametric Surface Plasmon Resonance (MP-SPR). The results confirmed that the complexes of cellulase and PSS were conjugated and the negatively supercharged complexes reduced non-productive binding effectively along with the improved saccharification efficiency. The thickness of PSS required to block the binding sites of cellulase film was less than half of that of LS, and the PSS adlayer on cellulase film is also more hydrated and with a much lower shear modulus than LS adlayer. Conclusions PSS as LS analogue is effective for enhancing the biomass enzymatic saccharification of GL-pretreated poplar. PSS exhibited a severer inhibition on the enzymatic saccharification of pure cellulose, while a more positive effect on bioconversion of lignocellulose (GL-P) than LS. In addition, a much lower dosage is required by PSS. The dynamic enzymatic hydrolysis indicated PSS could prolong the processive activity of cellulase. The valid data stemmed from QCM and SPR expressed that PSS bound to cellulases and the as-formed complexes reduced the non-productive adsorption of cellulase onto substrate lignin more efficiently than LS due to its flexible skeleton and highly hydrated structure. Therefore, PSS is a promising alternative promoting agent for lignocellulose saccharification. From another perspective, the synthetic lignin mimics with controllable structures enable us to reach an in-depth understanding of the promotion mechanism of soluble lignins on enzymatic saccharification.

show abstract

Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring

Cited by 33 publications

References 58 publications

Polystyrene Sulfonate is Effective for Enhancing Biomass Enzymatic Saccharification Under Green Liquor Pretreatment in Bioenergy Poplar

Polystyrene Sulfonate is Effective for Enhancing Biomass Enzymatic Saccharification Under Green Liquor Pretreatment in Bioenergy Poplar

Kinetics and Thermodynamics of Hemicellulose Adsorption onto Nanofibril Cellulose Surfaces by QCM-D

Polystyrene sulfonate is effective for enhancing biomass enzymatic saccharification under green liquor pretreatment in bioenergy poplar

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