Interactions of Chemicals and Metal Ions with Proteins and Role for Immune Responses

Martin, Stefan F.; Merfort, Irmgard; Thierse, Hermann‐Josef

doi:10.2174/138955706776073493

Cited by 28 publications

(28 citation statements)

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“…Allergic contact dermatitis (ACD), a common occupational and environmental health problem, is a delayed-type hypersensitivity reaction induced by small reactive chemicals following skin contact [19,35]. A critical step in the induction of ACD is the activation and subsequent migration of immature dendritic cells (DC) of the skin, namely the Langerhans cells (LC).…”

Section: Introductionmentioning

confidence: 99%

Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 expression in dendritic cells: relevance of different signalling pathways

et al. 2009

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Thioredoxin-1 is a ubiquitous protein involved in phenotypical and functional changes in dendritic cells (DC). We investigated the eVect of lipopolysaccharide (LPS), skin sensitizers, and irritants on thioredoxin-1 by Western blot and immunoXuorescence and on mRNA by real-time PCR. As DC models, we used a skin DC line and DC derived from human blood monocytes. We observed that all tested chemicals increased thioredoxin-1 expression, which is only transient for irritants, being the strongest eVect observed for LPS (63 § 15%). To address the involvement of thioredoxin-1 in DC maturation, we analysed the eVect of an activator of thioredoxin-1 expression, hydrogen peroxide, on CD86 expression, a marker of DC maturation. We found that hydrogen peroxide increases thioredoxin-1 and CD86 expression reinforcing thioredoxin-1 involvement in DC maturation. Because mitogen-activated protein kinases and PI3K are activated upon DC maturation, we also analysed their involvement in thioredoxin-1 modulation. We veriWed that LPS-induced upregulation of thioredoxin-1 expression was dependent on PI3K pathway.

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Section: Introductionmentioning

confidence: 99%

Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 expression in dendritic cells: relevance of different signalling pathways

et al. 2009

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“…Surprisingly, only a limited number of proteins seem susceptible to hapten modification. Among the identified proteins are many that are associated with innate immune and stress responses as well as with antigen processing such as heat shock proteins [64][65][66]. Moreover, by applying recently established quantitative proteomic technologies to human skin cells, novel chemically regulated protein patterns have been discovered, which also may support the development of allergen-specific biomarkers in in vitro testing [64].…”

Section: Topic 1: Chemical-reactive T Cellsmentioning

confidence: 99%

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

Martin

Esser

Schmucker

et al. 2010

Cell. Mol. Life Sci.

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133

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Chemicals can elicit T-cell-mediated diseases such as allergic contact dermatitis and adverse drug reactions. Therefore, testing of chemicals, drugs and protein allergens for hazard identification and risk assessment is essential in regulatory toxicology. The seventh amendment of the EU Cosmetics Directive now prohibits the testing of cosmetic ingredients in mice, guinea pigs and other animal species to assess their sensitizing potential. In addition, the EU Chemicals Directive REACh requires the retesting of more than 30,000 chemicals for different toxicological endpoints, including sensitization, requiring vast numbers of animals. Therefore, alternative methods are urgently needed to eventually replace animal testing. Here, we summarize the outcome of an expert meeting in Rome on 7 November 2009 on the development of T-cell-based in vitro assays as tools in immunotoxicology to identify hazardous chemicals and drugs. In addition, we provide an This article is dedicated to the memory of our colleague and friend Reinhard Wanner, died 2 April 2010. Cellular and Molecular Life Sciences overview of the development of the field over the last two decades.

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“…Therefore, the new singular dye protein-labeling approach has been chosen, which allows a complete protein matching by using Delta2D software. Human keratinocytes were stimulated with nickel, representing the most common human contact allergen [29][30][31], and singular DY-maleimide-labeled (DY-505-MAL) 2-DE gel-to-gel analyses (stimulated versus control) were performed (n 5 3). Comparative Delta2D matching of SDPL samples -preferentially compared between different biological samples in one 2-DE run (Ettan chamber for 12 gels; GE) -demonstrated a qualitatively (Fig.…”

Section: Matching Of Differentially/singular Dymaleimide Dye-labeled mentioning

confidence: 99%

Quantitative DY‐maleimide‐based proteomic 2‐DE‐labeling strategies using human skin proteins

et al. 2009

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Sensitive differential proteomic analysis is challenging and often limited by distinct labeling or tagging strategies. In this study, we have examined the sensitivity, linearity, and photophysical properties of novel protein labeling DY-maleimide dyes (DY-505-MAL, DY-555-MAL and DY-635-MAL). All MS compatible DY-maleimide dyes exhibited excellent emission spectra, high sensitivity, and high linearity, when applied to standard 1-DE protein analysis. Correspondingly, 2-DE analysis of DY-635-MAL or DY-505-MAL maximal-labeled human keratinocyte proteins displayed remarkably high sensitivity. Compared with a standard fluorescent protein stain, DY-635-MAL or DY-505-MAL 2-DE analysis demonstrated equally high spot quality with an overall increase in the number of spots detectable (up to threefold higher;41000 spots/gel). However, as determined with a FLA-5100 imaging system, comparative MultiGauge, and Delta2D analysis, not all DY-maleimide dyes possessed DIGE compatible fluorescent emission properties. However, DY-505-MAL and DY-635-MAL were found to be suitable for more complex, time and gel intensive, focused multiplexing analyses. Notably -as demonstrated with allergen-stimulated human skin proteins -defined, singular DY-maleimide dye protein labeling (SDPL) allows high quality, time saving, simple, and reliable differential proteomic examination.

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Interactions of Chemicals and Metal Ions with Proteins and Role for Immune Responses

Cited by 28 publications

References 100 publications

Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 expression in dendritic cells: relevance of different signalling pathways

Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 expression in dendritic cells: relevance of different signalling pathways

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

Quantitative DY‐maleimide‐based proteomic 2‐DE‐labeling strategies using human skin proteins

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