Interactions of graphene and graphene oxide with proteins and peptides

Abstract

This review selectively describes the recent progress in the interactions of proteins (enzymes) and shortchain peptides with graphene and graphene oxide (GO). Particularly, the advances of the immobilization mechanisms of enzymes on graphene and GO, the catalytic properties of the immobilized enzymes, and their applications are summarized in detail. The interfacings of the peptides with graphene and GO, the as assembled conjugates, and their potential applications are discussed briefly. The possible ongoing development for the assembly of conjugates of graphene and GO with proteins and peptides in a controlled manner is speculated upon.

Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing

“…The interconnected two-dimensional (2D) substrates could be served as a platform for enzyme immobilization through non-covalent adsorption and covalent binding [26]. Zeta potential measurements showed that after the surface modification with Fe 3 O 4 nanoparticles, the Zeta potential value of GO dramatically changed from −43.7 mV to +31.5 mV.…”

Increased thermal stability of cold-adapted esterase at ambient temperatures by immobilization on graphene oxide

“…Several studies have reported the immobilization of peptides and enzymes on GO via covalent bonding or non-covalent interactions (Pavlidis et al, 2012;Su et al, 2012;Zhang et al, 2010;Zhang et al, 2011;Zhang et al, 2013). Alkaline protease immobilized on GO exhibited enhanced thermal stability at its optimum temperature of 60°C (Su et al, 2012).…”

In situ synthesized rGO–Fe3O4 nanocomposites as enzyme immobilization support for achieving high activity recovery and easy recycling

“…When the pH value of the initial GO suspension increases via the ammonia addition, the Fe 2+ ions co−precipitate with the acquired Fe 3+ ions into Fe3O4, leading to the formation of rGO−Fe3O4 nanocomposites. Subsequently, the CAT is directly adsorbed on the surface of as-prepared rGO−Fe3O4 nanocomposites via the multiple interactions (hydrogen bonding and hydrophobic interaction) between CAT and rGO−Fe3O4 surface to acquire the rGO−Fe3O4−CAT[17,36]. As for the biomimetic adhesion, a polydopamine (pDA) layer was firstly functionalized and wrapped on the surface of rGO−Fe3O4 nanocomposites (the preparation process of rGO−Fe3O4−pDA is presented in the Supplementary data).…”