Interactions of herbal extract combinations against free radical scavenging activity

Prakash, Om; Singh, Gyanendra; Singh, Rohit; Madan, Swati; Mathur, Satish C.

doi:10.1080/13880200902939267

“…This study showed that the E225 group exhibited the best results for improving all parameters. This is in accordance with Prakash et al [35], who proved that the four extracts together were more effective than a single extract or combination of two extracts against free radicals. Even though the components of the extract are the same, different amounts of each extract produce different results in reducing the levels of lipids, lipoproteins, and oxidative stress [36].…”

Section: Discussionsupporting

confidence: 92%

Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

Susilowati

¹

,

Setiawan

²

2020

Vet World

3

0

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Background and Aim: Hyperlipidemia is an important risk factor for cardiovascular disease. The use of statins has adverse side effects that result in oxidative stress disorders. The objective of this study was to investigate the antihyperlipidemic effect of a combination of Cinnamomum burmannii and Eleutherine palmifolia extract in high-fat diet (HFD)-induced hyperlipidemia mice. Materials and Methods: Mice were divided into eight groups (n=4): Control group or healthy mice (normal), HFD-induced hyperlipidemic mice without any treatment (CE0), HFD-induced hyperlipidemic mice treated with 3.6 mg/kg body weight (BW) atorvastatin (atorvastatin), and HFD-induced hyperlipidemic mice treated with a combination of C. burmannii and E. palmifolia in the following ratios: 300:0 (C300), 225:75 (C225), 150:150 (CE150), 75:225 (E225), and 0:300 (E300). Mice were fed a HFD for 4 months to induce hyperlipidemia. Total cholesterol, cholesterol oxidase-peroxidase aminophenazone (CHOD-PAP), triglyceride-glycerine, and fat serum were analyzed with colorimetric method. The measurement of superoxide dismutase was done with the xanthine oxidase method and malondialdehyde measurement was done with the thiobarbituric acid method. Results: Results showed an increase in antihyperlipidemic characteristics as the concentration of E. palmifolia extract (p<0.05) increased. Duncan's multiple range test also showed an increase in anti-stress oxidation as the concentration of C. burmannii extract (p<0.05) increased. Conclusion: The E225 group showed the most potential as a safe, antihyperlipidemic agent characterized by improvement in lipid profile and antioxidant balance.

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“…This is in accordance with Prakash et al . [ 35 ], who proved that the four extracts together were more effective than a single extract or combination of two extracts against free radicals. Even though the components of the extract are the same, different amounts of each extract produce different results in reducing the levels of lipids, lipoproteins, and oxidative stress [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

Susilowati

¹

,

Setiawan

²

2020

View full text Add to dashboard Cite

Background and Aim: Hyperlipidemia is an important risk factor for cardiovascular disease. The use of statins has adverse side effects that result in oxidative stress disorders. The objective of this study was to investigate the antihyperlipidemic effect of a combination of Cinnamomum burmannii and Eleutherine palmifolia extract in high-fat diet (HFD)-induced hyperlipidemia mice. Materials and Methods: Mice were divided into eight groups (n=4): Control group or healthy mice (normal), HFD-induced hyperlipidemic mice without any treatment (CE0), HFD-induced hyperlipidemic mice treated with 3.6 mg/kg body weight (BW) atorvastatin (atorvastatin), and HFD-induced hyperlipidemic mice treated with a combination of C. burmannii and E. palmifolia in the following ratios: 300:0 (C300), 225:75 (C225), 150:150 (CE150), 75:225 (E225), and 0:300 (E300). Mice were fed a HFD for 4 months to induce hyperlipidemia. Total cholesterol, cholesterol oxidase-peroxidase aminophenazone (CHOD-PAP), triglyceride-glycerine, and fat serum were analyzed with colorimetric method. The measurement of superoxide dismutase was done with the xanthine oxidase method and malondialdehyde measurement was done with the thiobarbituric acid method. Results: Results showed an increase in antihyperlipidemic characteristics as the concentration of E. palmifolia extract (p<0.05) increased. Duncan's multiple range test also showed an increase in anti-stress oxidation as the concentration of C. burmannii extract (p<0.05) increased. Conclusion: The E225 group showed the most potential as a safe, antihyperlipidemic agent characterized by improvement in lipid profile and antioxidant balance.

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“…The scavenging rate (DPPH%) values and IC50 values of a single combined herb system were determined according to the methods mentioned earlier. Antagonism, additive or synergistic effect were assessed with the interaction index from the data derived experimentally according to the method by Tallarida (2002) and Prakash et al (2009) with some changes.…”

Section: Dpph Assay Of Two-two Combined System Of Extracts Of Fsdmentioning

confidence: 99%

“…The interaction index was originated from the literature (Prakash et al, 2009) and was modified by the consideration that the interaction effect is not same as the ratio of each herb is different. Consequently, the mass percent and IC50 were added into the formula and it was denoted by γ.…”

Section: Interaction Indexmentioning

confidence: 99%

Free radical scavenging activity of Chinese traditional four substances decoction (FSD) extracts

Wang¹

2012

Afr. J. Pharm. Pharmacol.

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This article examined the interactive effect among herbs extracts in combined use, compared with the effect of individual herbal extracts on 1,1-diphenyl picryl hydrazyl (DPPH). The Chinese traditional prescription, four substances decoction (FSD) consisted of 25 g of Angelica sinensis (Umbelliferae, AS), 6 g of Ligusticum chuanxiong Hort. (Umbelliferae, LCH), 10 g of Paeonia loactiflora Pall. (Paeoniaceae, PCP), and 15 g of Radix rehmanniae preparata (Scrophulariaceae, RRP). Each herb was quantitatively mixed according to the FSD prescription ratio. The interaction between the herbal extract in two-two combination way were estimated using a spectrophotometer. The results showed the strongest synergistic effect in LCH and PLP system, with IC 50 0.25 mg/ml, and the additive effect was obvious in the AS and LCH combined system. The other combined system showed additive effect or sub-additive effect. This implied that the combined system effect have a close relationship with the single component activity.

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Interactions of herbal extract combinations against free radical scavenging activity

Cited by 6 publications

References 18 publications

Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

Free radical scavenging activity of Chinese traditional four substances decoction (FSD) extracts

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