Interactions of Human Endothelial Cells with Gold Nanoparticles of Different Morphologies

Bartczak, Dorota; Muskens, Otto L.; Nitti, Simone; Sánchez-Elsner, Tilman; Millar, Timothy M.; Kanaras, Antonios G.

doi:10.1002/smll.201101422

Cited by 120 publications

(113 citation statements)

References 53 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…A number of studies with AuNPs have shown that size, shape, concentration, and surface modification of the NPs affect the interaction with cells and may lead to varying degrees of internalization and cytotoxicity. [3][4][5][6] Modifying the surface of AuNPs with polyethylene glycol (PEG) has been demonstrated to prolong the circulation time in vivo, 7,8 this being explained by a reduced renal filtration 9 In addition, it has been demonstrated that 15 nm PEGylated AuNPs do not affect platelet aggregation, nor do they induce pro-inflammatory cytokines in endothelial cells. 10 In previous studies it was also shown that PEGylated AuNPs of different sizes did not reduce cell viability or induce cellular stress and were internalized in low amounts by various microvascular endothelial cells compared to the same AuNPs functionalized with various surface polymers.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

et al. 2017

View full text Add to dashboard Cite

a PEGylated gold nanoparticles (AuNPs) have an extended circulation time after intravenous injection in vivo and exhibit favorable properties for biosensing, diagnostic imaging, and cancer treatment. No impact of PEGylated AuNPs on the barrier forming properties of endothelial cells (ECs) has been reported, but recent studies demonstrated that unexpected effects on erythrocytes are observed. Almost all studies to date have been with static-cultured ECs. Herein, ECs maintained under physiological cyclic stretch and flow conditions and used to generate a blood-brain barrier model were exposed to 20 nm PEGylated AuNPs. An evaluation of toxic effects, cell stress, the release profile of pro-inflammatory cytokines, and blood-brain barrier properties showed that even under physiological conditions no obvious effects of PEGylated AuNPs on ECs were observed. These findings suggest that 20 nm-sized, PEGylated AuNPs may be a useful tool for biomedical applications, as they do not affect the normal function of healthy ECs after entering the blood stream.

show abstract

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Comparison with TEM showed the capability of measuring individual hollow gold NP as small as 50 nm with a short integration time per image of 0.2 s. The system also allows identification of these individual hollow gold NP in fixed cell preparations, at illumination intensities < 0.5 W/cm 2 . Such intensities have been shown to result in minimal toxicity for cells incubated with metal NPs 18 . This advantage of using a scanned illumination source will be important for future live cell studies of nanoparticle uptake and processing in cells, where phototoxicity is currently a limitation.…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, the application of a broad spectrum of irradiation at once can produce a significant heating of the particle, which is especially unfavorable for in-vitro studies of sensitive biological systems. 17,18 Further, applications involving NP counting and/or tracking favor a wide-field approach in combination with spectroscopic characterization. Various different approaches have been presented based on hyperspectral imaging where individual spectral components are filtered.…”

Section: Introductionmentioning

confidence: 99%

“…Hollow gold particles are of particular interest for biomedical applications because their LSPR falls in the biological near IR window. 18 Additionally biological molecules can be selectively encapsulated within and released from the hollow particle. 24 In our current work, scattering spectra of colloidal hollow gold nanoparticles and particle clusters were measured and correlated with TEM images, and theoretical spectra were produced using finite element analysis to interpret the observed resonances.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Fairbairn

Christofidou

Kanaras

et al. 2013

Phys. Chem. Chem. Phys.

Self Cite

View full text Add to dashboard Cite

Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 µm 2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments.

show abstract

“…GALA's transition from random coil to an amphiphilic α-helix roughly occurs when the pH is decreased from 7 to 5, the pH of inflection between the two conformational states was reported to be near pH 6. [9][10][11][12] At low pHs the Glu sites are protonated and uncharged. The hydrophobic periodicity of the EALA repeats allows the peptide to fold into a stable amphiphilic helix with hydrophobic leucine and alanine sites on one, and hydrophilic glutamic acids on the other side of the helix (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

Sticky water surfaces: Helix–coil transitions suppressed in a cell-penetrating peptide at the air-water interface

Schach

Globisch

Roeters

et al. 2014

The Journal of Chemical Physics

View full text Add to dashboard Cite

Sticky water surfaces: Helix-coil transitions suppressed in a cell-penetrating peptide at the air-water interface Schach, D.; Globisch, C.; Roeters, S.J.; Woutersen, S.; Fuchs, A.; Weiss, C.K.; Backus, E.H.G.; Landfester, K.; Bonn, M.; Peter, C.; Weidner, T. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. GALA is a 30 amino acid synthetic peptide consisting of a Glu-Ala-Leu-Ala repeat and is known to undergo a reversible structural transition from a disordered to an α-helical structure when changing the pH from basic to acidic values. In its helical state GALA can insert into and disintegrate lipid membranes. This effect has generated much interest in GALA as a candidate for pH triggered, targeted drug delivery. GALA also serves as a well-defined model system to understand cell penetration mechanisms and protein folding triggered by external stimuli. Structural transitions of GALA in solution have been studied extensively. However, cell penetration is an interfacial effect and potential biomedical applications of GALA would involve a variety of surfaces, e.g., nanoparticles, lipid membranes, tubing, and liquid-gas interfaces. Despite the apparent importance of interfaces in the functioning of GALA, the effect of surfaces on the reversible folding of GALA has not yet been studied. Here, we use sum frequency generation vibrational spectroscopy (SFG) to probe the structural response of GALA at the air-water interface and IR spectroscopy to follow GALA folding in bulk solution. We combine the SFG data with molecular dynamics simulations to obtain a molecularlevel picture of the interaction of GALA with the air-water interface. Surprisingly, while the fully reversible structural transition was observed in solution, at the water-air interface, a large fraction of the GALA population remained helical at high pH. This "stickiness" of the air-water interface can be explained by the stabilizing interactions of hydrophobic leucine and alanine side chains with the water surface. © 2014 AIP Publishing LLC. [http://dx

show abstract

Interactions of Human Endothelial Cells with Gold Nanoparticles of Different Morphologies

Cited by 120 publications

References 53 publications

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Sticky water surfaces: Helix–coil transitions suppressed in a cell-penetrating peptide at the air-water interface

Contact Info

Product

Resources

About