Interactions of hydroxy-s-triazines with sodium dodecyl sulfate-micelles investigated by micellar capillary electrophoresis

Freitag, D.; Schmitt‐Kopplin, Philippe; Simon, Rupert; Kaune, A.; Kettrup, A.

doi:10.1002/(sici)1522-2683(19990601)20:7<1568::aid-elps1568>3.0.co;2-w

Cited by 15 publications

(4 citation statements)

References 51 publications

(52 reference statements)

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“…At low micelle concentrations there is a linear relationship between k and SDS concentration ( C sf ): where ν is surfactant molar volume (0.25 l mol −1 for SDS),14 P mw is the partition coefficient of a solute between aqueous phase and micelles, V mc is the micellar volume and V aq is the aqueous phase volume.…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of the MECC experimental conditions showed that different degrees of ionization and a keto‐enol tautomerism of each xanthine were taking place. In fact, dissociation equilibria and keto‐enol tautomerism by micellar capillary electrophoresis of another family of compounds, the 4,6‐diamino‐ s ‐triazines, have been previously reported 14…”

Section: Introductionmentioning

confidence: 99%

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Micellar electrokinetic capillary chromatography of methylxanthines‐containing beverages: discussion of the molecular species involved

2004

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Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mg l −1 , the limit of quantitation was 2.5 mg l −1 and the work range was 2.5-300 mg l −1 . This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic capillary chromatography of methylxanthines‐containing beverages: discussion of the molecular species involved

2004

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“…Sudan III has been widely used as micellar marker because it is a very hydrophobic solute and it was assumed to be completely solubilized into the micelle [22,23,[33][34][35][36]. Other compounds used as micellar markers are phenanthrene [37], anthracene [38], octylbenzene [10,11], and dodecanophenone [8,[12][13][14]39]. The complete solubilization of these compounds is based on their interaction with the micelle.…”

Section: Determination Of the Micelle Migration Timementioning

confidence: 99%

Solute-solvent interactions in micellar electrokinetic chromatography: IV. Characterization of electroosmotic flow and micellar markers

et al. 2002

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A wide study of the compounds and procedures mostly used to determine the electroosmotic flow (EOF) and micelle elution times has been done in seven different micellar electrokinetic chromatography (MEKC) systems. These systems are formed from mixtures of an aqueous buffer with the surfactants sodium dodecyl sulfate, lithium dodecyl sulfate, lithium perfluorooctane sulfonate, sodium cholate, sodium deoxycholate, tetradecyltrimethylammonium bromide and hexadecyltrimethylammonium bromide. The solvation parameter model has been used to evaluate the usefulness of the compounds studied as EOF or micellar markers in each of the seven MEKC systems. It is demonstrated that methanol, acetonitrile and formamide are the best EOF markers, and that dodecanophenone is the best micellar marker.

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“…The electrophoretic mobility of charged substances is fundamentally important and is affected by variable experimental conditions (dissociation constants p K , isoelectric points p I s, hydrophobicities log( p ), charge, binding constants) . Since the introduction of CE in the 1960s, different models for better understanding of CZE were developed to evaluate physical–chemical parameters . Semi‐empirical models have been described to predict the electrophoretic mobilities of peptides and to obtain information on their amide groups .…”

Section: Introductionmentioning

confidence: 99%

Capillary electrokinetic fractionation mass spectrometry (CEkF/MS): Technology setup and application to metabolite fractionation from complex samples coupled at‐line with ultrahigh‐resolution mass spectrometry

Harir

Gougeon

et al. 2014

Electrophoresis

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Capillary electrokinetic fractionation (CEkF) is investigated as a new, simple, and robust approach for semipreparative and analytical sample analysis based on pKa -dependant pH-driven electrophoretic mobility. CEkF was optimized with contactless conductivity detection and conducted with 10 kV reverse voltage for 10 min, then coupled on/at-line to ESI/MS. We propose a semi-empirical model with 14 representative compounds based on the correlation between sample/medium pH regulating the partial charge, the electrokinetic loading of the capillary and intensity (I) of analytes. According to the model, an empirical function (I = f (pH)) could be derived to calculate the acid dissociation constant (pKa ) of various model compounds based on their pH-dependant MS intensity profiles with the RSD < 4.05. Using the ultrahigh-resolution of ion cyclotron resonance Fourier transform MS, the pKa model was further illustrated in real samples into the structure prediction of important compounds in wine over two vintages. The established CEkF was successfully used to selectively fractionate sulfur compounds from the complex wine samples at pH 1.66. The proposed CEkF approach should allow in the future the simultaneous pKa evaluation of multiple constituents without complicated separation out of a complex mixture in metabolomics or environmental chemistry.

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Interactions of hydroxy-s-triazines with sodium dodecyl sulfate-micelles investigated by micellar capillary electrophoresis

Cited by 15 publications

References 51 publications

Micellar electrokinetic capillary chromatography of methylxanthines‐containing beverages: discussion of the molecular species involved

Micellar electrokinetic capillary chromatography of methylxanthines‐containing beverages: discussion of the molecular species involved

Solute-solvent interactions in micellar electrokinetic chromatography: IV. Characterization of electroosmotic flow and micellar markers

Capillary electrokinetic fractionation mass spectrometry (CEkF/MS): Technology setup and application to metabolite fractionation from complex samples coupled at‐line with ultrahigh‐resolution mass spectrometry

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