Interactions of hydroxyapatite and fluorapatite particles on human osteoarthritis type B synoviocytes: Effects on interleukin-1? levels and lipoxygenase pathways

Abstract

We investigated the effects of the biomaterials hydroxyapatite (HAP) and fluorapatite (FAP) on cultured human osteoarthritis type B synoviocytes by analyzing interleukin-1 alpha (IL-1 alpha) production and arachidonic acid metabolism via lipoxygenase pathways. A portion of opsonized particles was endocytosed and was found in numerous phagolysosomes in human synoviocyte cytoplasms. The present study demonstrates that HAP and FAP calcined at 700 degrees C induced a decrease in IL-1 alpha production but markedly decreased the synthesis of lipoxygenase products after 1-month incubation with the particles. This model will allow us to study the possible inflammatory response (arachidonic acid metabolism, proinflammatory cytokines) that can be induced by any biomaterials used in orthopedics.

Synoviocytes are more sensitive than cartilage to the effects of minocycline and doxycycline on IL-1α and MMP-13-induced catabolic gene responses

“…To the authors' knowledge, there are no studies comparing the biological responsiveness of type A to type B synoviocytes to any treatment, and therefore the impact of losing type A synoviocytes on the results of the present study cannot be determined. In contrast, there are many studies elucidating the role of isolated type B synoviocytes in the pathogenesis of RA and OA through their secretion of degratory enzymes, inflammatory mediators, and innate immune responses23–25 to which this study can be compared.…”

The inflammatory potential of biphasic calcium phosphate granules in osteoblast/macrophage co-culture

“…These two cell phenotypes have been established as the major components of the cellular response that result in the formation of a fibrous sheath in the place of viable bone tissue [17]. These studies have also highlighted numerous growth factors and cytokines, which control the rate of osteoclast and macrophage resorption of bone tissue [16,18,19]. Interleukin-1 (IL-1) stimulates both osteoclast formation and the associated bone resorbing capacity of these cells.…”

Effects of alumina and zirconium dioxide particles on arachidonic acid metabolism and proinflammatory interleukin production in osteoarthritis and rheumatoid synovial cells

“…By contrast, RA is characterised by chronic joint inflammation and concomitant destruction of cartilage and bone. By comparing recent results with hydroxyapatite and fluorapatite, 27 we sought to determine if alumina (Al 2 O 3 )…”