Interactions of Immunoglobulins G and M in the Detection of the Mammalian C-Type Virus Cross-Reactive Antigen

Oroszlan, Stephen; Bova, D.; Toni, Robert; Gilden, Raymond V.

doi:10.1126/science.176.4033.420

Cited by 21 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent work (10,12,(23)(24)(25)(26) has indicated that mammalian (27). It is reasonable to suppose that rabbits immunized with such viral preparations may produce an additional antibody against cell-surface antigens that contaminate the density-purified virus preparation used for immunization.…”

Section: Methodsmentioning

confidence: 99%

Common Cell-Surface Antigen Associated with Murine and Feline C-Type RNA Leukemia Viruses

Yoshiki

Mellors

Hardy

1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A new common cell-surface antigen associated with murine and feline C-type RNA leukemia viruses was demonstrated by the use of rabbit antiserum against feline leukemia virus and the indirect membrane immunofluorescence test. Common cell-surface antigen was found in all leukemias of all strains of mice tested, in normal lymphoid tissues of Gross-positive (high incidence of leukemia) mouse strains AKR, AKR * H-2b, C58, and NZB, in cultured rat fibroblasts infected with Rauscher virus, in cultured feline fibroblasts infected with feline leukemia virus, and in spontaneous feline lymphosarcoma. The antigen was not demonstrable in normal adult and fetal tissues of Gross-negative mouse strains or in tissues and cultured fibroblasts derived from normal rats and normal cats. The immunoferritin study of murine leukemia cells revealed that the antigen was located on the cell surface in discrete areas; budding and C-type RNA viral envelope was not labeled as antigen site. The distribution of common cell-surface antigen on murine and feline leukemias, as well as on normal lymphoid tissues of Gross-positive mouse strains, indicates the presence of an antigen distinct from any cell-surface antigen heretofore shown to be associated with, or specified by, mammalian C-type RNA viruses.

show abstract

Section: Methodsmentioning

confidence: 99%

Common Cell-Surface Antigen Associated with Murine and Feline C-Type RNA Leukemia Viruses

Yoshiki

Mellors

Hardy

1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…The virus isolated from MSB-1 cells, previously designated as M-MSV(RaLV) based on immunologic (26) and molecular hybridization methodology (5), appears to synthesize at least three distinct virus-related RNA subunits; 35S of RaLV, 30 S related to KiSV or RaLV, and 30 S of M-MSV. However, the possibility that a 30S RNA molecule with both KiSV-and M-MSV-related sequences exists has not yet been excluded.…”

mentioning

confidence: 99%

Sarcoma-Virus-Related RNA Sequences in Normal Rat Cells

Tsuchida

Gilden

Hatanaka

1974

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

A rat type C virus spontaneously activated from the NRK (normal rat kidney) cell line was found to have two major size classes of viral RNA subunits sedimenting at 35 and 30 S. Virus-producing cells contained both RNA species, while normal "virus-free" rat cells contained primarily virus-specific 30S RNA species. A DNA transcript, specific for Kirsten sarcoma virus, pre- Three isolates, previously considered to be murine sarcoma viruses, have been studied by nucleic acid hybridization techniques during the past 2 years. Two of these isolates, the Harvey (1) and Kirsten (2) strains, arose on rat passage of mouse leukemia viruses (Moloney and Kirsten strains, respectively), and the third, the Moloney strain (3), arose on mouse passage of the Moloney leukemia virus (M-MuLV). Based on minimal hybridization between rat and mouse type C viruses (4, 5), it was possible to show that the Kirsten and Harvey sarcoma viruses derived their sarcoma-specific nucleic acid sequences from the rat type C virus (4, 6), while the Moloney strain (M-MSV) was exclusively of mouse origin (4, 5).We have previously reported preparation of KiSV-specific DNA transcripts that did not hybridize with RNA of helper mouse virus (7,27 spontaneously virus-producing rat cells has now shown KiSVspecific sequences associated with a 30S RNA in both cells, whereas virus-producing rat cells produce an additional 35S RNA subunit that is not related to KiSV. MATERIALS AND METHODSCells and Media. The following rat cell lines were used: NRK-9 (RaLV-NRK), a normal rat kidney (NRK) cell line producing endogenous rat type C virus (RaLV) (8); MSB-1 (9), a rat cell transformed by M-MSV producing M-MSV-(RaLV) (5); a Fisher rat embryo cell (5053) The following mouse cells were used: BALB/3T3 (A-31), and a derivative K-234, a BALB/3T3 cell line transformed by KiSV (NP cell) (12, 13); M-58-2, a mutant cell line derived from the NP cell after short-term treatment with 5'-bromodeoxyuridine (BrdU) (14, 15); 58-2T, a cell line established from a slowly growing tumor in a BALB/c mouse that was inoculated with M-58-2 cells (7); Ki-MuLV-producing NIH/ 3T3 cell (Ki-MuLV-NJH/3T3), obtained from Dr. V. Klement (Ki-MuLV had not been passaged in rat); and JLS-V9 infected with Rauscher strain of MuLV (R-MuLV) (16).A nonproducer hamster cell transformed by M-MSV (HT-1) was also used (17).All cells were grown in Eagle's medium with 10% (v/v) fetal bovine serum and antibiotics.Preparation of RNA. Cell RNA was prepared as described (18), by the hot phenol method. The concentration was determined by the orcinol reaction (19). Synthesis of Virus-Specific DNA. Viral [3H]DNA was prepared by the endogenous RNA-directed DNA polymerase reaction with RaLV, R-MuLV, M-MSV(MuLV), Ki-MuLV, and the virus produced by 58-2T cells, 58-2T virus. These viruses were purified as described (11). Reaction mixtures, generally 5 ml, contained 1-2 ml of purified virus solution (0.1-1.0 mg/ml of protein), 0.1 M glycine-NaOH buffer, (pH 8.0), 10 mM dithiothreitol, 0.05 mM each of dATP, dGTP, and d...

show abstract

“…1 1]. In some experiments sera were separated into IgG-and IgM-containing fractions or treated with 2-mercaptoethanol as previously described [12].…”

Section: Methodsmentioning

confidence: 99%

“…These polypeptides react with rat anti-MuLV IEFP serum indicating that these four, and probably all, species p30s share an antigenically similar core not readily digested by trypsin. In addition, this study presents data demonstrating that some of the unusual immunological prop erties of mammalian p30s [12] are retained on the MuLV IEFP.…”

mentioning

confidence: 99%

An Antigenically Related Tryptic Polypeptide from Several Mammalian Type C RNA Virus p30s

Davis¹,

Gilden²,

Oroszlan³

1975

Intervirology

Self Cite

View full text Add to dashboard Cite

Limited tryptic digestion of p30 antigen (the major internal viral protein) of type C viruses isolated from hamster, rat, and gibbon ape yielded a polypeptide fragment with a molecular weight of approximately 10,000 daltons. Some antigenic determinants with interspecies specificity were retained on these polypeptides, which in serological tests cross-reacted with antibody produced previously against a similar fragment obtained from mouse p30. Exhaustive trypsinization resulted in further fragmentation with concomitant loss of serologic activity. The results suggest that all mammalian type C virus p30s have an antigenically related polypeptide core which is not readily digested by trypsin.

show abstract

Interactions of Immunoglobulins G and M in the Detection of the Mammalian C-Type Virus Cross-Reactive Antigen

Cited by 21 publications

References 10 publications

Common Cell-Surface Antigen Associated with Murine and Feline C-Type RNA Leukemia Viruses

Common Cell-Surface Antigen Associated with Murine and Feline C-Type RNA Leukemia Viruses

Sarcoma-Virus-Related RNA Sequences in Normal Rat Cells

An Antigenically Related Tryptic Polypeptide from Several Mammalian Type C RNA Virus p30s

Contact Info

Product

Resources

About