Interactions of Lecithinized Superoxide Dismutase with Serum Proteins and Cells

Ishihara, Tsutomu; Nara, Shunsuke; Mizushima, Tohru

doi:10.1002/jps.24031

Cited by 10 publications

(12 citation statements)

References 44 publications

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“…Some methods, such as binding to lecithin (Ishihara, Nara, & Mizushima, 2014), liposomal trapping (Rengel et al, 2002), or pegylation (Veronese, Caliceti, Schiavon, & Sergi, 2002) have been proved to increase the efficacy of some antioxidant enzymes like SOD. Although these improvements seem to increase the enzyme activity over their natural counterparts, there are still some shortcomings.…”

Section: Nano Formulated Antioxidant Enzymesmentioning

confidence: 99%

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Panahi

Rahimi

et al. 2020

Journal Cellular Physiology

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Mesenchymal stem cells (MSCs) are earmarked as perfect candidates for cell therapy and tissue engineering due to their capacity to differentiate into different cell types. However, their potential for application in regenerative medicine declines when the levels of the reactive oxygen and nitrogen species (RONS) increase from the physiological levels, a phenomenon which is at least inevitable in ex vivo cultures and air-exposed damaged tissues. Increased levels of RONS can alter the

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Section: Nano Formulated Antioxidant Enzymesmentioning

confidence: 99%

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Panahi

Rahimi

et al. 2020

Journal Cellular Physiology

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“…PC-SOD forms a complex with serum proteins such as albumin, whereas unmodified SOD does not. The cellular content, found in lysosomes, of PC-SOD is markedly higher when compared to unmodified SOD [17] . It has a low superoxide anion-scavenging activity into the mitochondria, but it accumulates in lymphocytes with a significant effect in scavenging extracellular superoxide anions [18] .…”

Section: Pc-sodmentioning

confidence: 81%

Lecithinized superoxide dismutase in the past and in the present: Any role in the actual pandemia of COVID-19?

Farella

Panza

Capozza

et al. 2021

Biomedicine & Pharmacotherapy

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The Coronavirus disease 19 (Covid-19) pandemic is devasting the public health: it is urgent to find a viable therapy to reduce the multiorgan damage of the disease. A validated therapeutic protocol is still missing. The most severe forms of the disease are related to an exaggerated inflammatory response. The pivotal role of reactive oxygen species (ROS) in the amplification of inflammation makes the antioxidants a potential therapy, but clinical trials are needed. The lecitinized superoxide dismutase (PC-SOD) could represent a possibility because of bioaviability, safety, and its modulatory effect on the innate immune response in reducing the harmful consequences of oxidative stress. In this review we summarize the evidence on lecitinized superoxide dismutase in animal and human studies, to highlight the rationale for using the PC-SOD to treat COVID-19.

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“…However, the MITC NPs, HACE/NPs and HACE/MITC NPs groups had significant effects. This may be due to the SOD activity of lecithin in liposome preparation material (Ishihara et al., 2014 ). Therefore, when MITC was encapsulated, its can effectively restore antioxidant enzyme activities reduced by UVB.…”

Section: Resultsmentioning

confidence: 99%

Anti-photoaging effects of flexible nanoliposomes encapsulated Moringa oleifera Lam. isothiocyanate in UVB-induced cell damage in HaCaT cells

et al. 2022

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Skin photoaging is premature skin aging damage that occurs after repeated exposure to ultraviolet (UV) radiation. Although isothiocyanates extracted from the moringa tree ( Moringa oleifera Lam. ) (MITC) exhibit excellent effects against skin photoaging, its application is restricted because of its characteristics, such as extremely low water solubility, bioavailability, and easy degradation. Currently, flexible nanoliposomes have gained increasing interest as a biocompatible polymer for applications such as transdermal drug delivery. We prepare amphiphilic hyaluronic acid (HA) conjugated with ceramide (CE) to modify nanoliposomes for MITC (HACE/MITC NPs) delivery. The HACE/MITC nanoparticles (NPs) are prepared and characterized for entrapment efficiency, particle size, polydispersity index, zeta potential, in vitro release, in vivo skin permeation, and in vitro protective effect of photoaging. The zeta potential of MITC NPs and HACE/MITC NPs is −24.46 mV and −24.93 mV, respectively. After modification of HACE, the entrapment efficient of MITC liposome increased from 62.54% to 70.67%, and the particle size decreased from 266.1 nm to 192.8 nm. In vivo skin permeation, permeated drug increased from 49.42 to 71.40%. Moreover, the results showed that the entrapment of MITC in nanoliposomes improves its stability, efficacy, and skin permeation. Further, HACE/MITC NPs are favorable for uptake by HaCaT cells without requiring changes in cell morphology, which significantly improves the activities of antioxidant enzymes, scavenges UVB-induced reactive oxygen species, protects skin from damage, and reduces MMP-1, MMP-3, and MMP-9 expression caused by radiation-induced photoaging. Our results strongly suggest that flexible nanoliposomes successfully improved the cell membrane permeation of MITC, and that anti-photoaging and HACE/MITC NPs can potentially be used as candidates for photoaging therapy.

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Interactions of Lecithinized Superoxide Dismutase with Serum Proteins and Cells

Cited by 10 publications

References 44 publications

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Lecithinized superoxide dismutase in the past and in the present: Any role in the actual pandemia of COVID-19?

Anti-photoaging effects of flexible nanoliposomes encapsulated Moringa oleifera Lam. isothiocyanate in UVB-induced cell damage in HaCaT cells

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