Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: Characterization of their biochemical and immunological properties

Hurk, Sylvia van Drunen Littel‐van den; Hurk, J V van den; Gilchrist, J. E.; Misra, Vikram; Babiuki, L.A.

doi:10.1016/0042-6822(84)90201-0

Cited by 135 publications

(106 citation statements)

References 28 publications

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“…These proteins are located on the surface of the virus and have both functional and immunological characteristics that render them attractive for vaccine development. They are the immunodominant antigens of BoHV-1 since they are the target of most neutralising antibodies [1,37,51] and the major antigens of the cellular immune response mounted after infection [11,19,26,39]. Glycoprotein gD is an essential protein of BoHV-1 that plays critical functions for the viral entry process.…”

Section: Introductionmentioning

confidence: 99%

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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-Bovine herpesvirus 1 (BoHV-1) has frequently been used as a model for testing parameters affecting DNA immunisation in large animals like cattle. However, the selection of target antigens has been poorly studied, and most of the experiments have been conducted in mice. In the present study, we demonstrated in cattle that a DNA vaccine encoding BoHV-1 glycoprotein gD induces higher neutralising antibody titres than vaccines encoding BoHV-1 gC. Additionally, we show that a DNA vaccine encoding a secreted form of gD induces a higher immune response than a vaccine encoding full-length gD. However, the enhanced immunogenicity associated with the secretion of gD could not be extended to the glycoprotein gC. The current study also describes for the first time the development and the evaluation of a DNA vaccine encoding the major tegument protein VP8. This construct, which is the first BoHV-1 plasmid vaccine candidate that is not directed against a surface glycoprotein, induced a high BoHV-1 specific cellular immunity but no humoral immune response. The calves vaccinated with the constructs encoding full-length and truncated gD showed a non-significant tenfold reduction of virus excretion after challenge. Those calves also excreted virus for significantly (p < 0.05) shorter periods (1.5 days) than the nonvaccinated controls. The other constructs encoding gC and VP8 antigens induced no virological protection as compared to controls. Altogether the DNA vaccines induced weaker immunity and protection than conventional marker vaccines tested previously, confirming the difficulty to develop efficient DNA vaccines in large species.BoHV-1 / DNA immunisation / gC / gD / VP8

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Section: Introductionmentioning

confidence: 99%

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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“…COS-7 cell supernatants containing tgD or tgD-CD154 were separated on 10% polyacrylamide gels under reducing or nonreducing conditions (27), and the proteins were transferred to nitrocellulose membrane (Millipore). The membrane was washed in TBST (0.15 M Tris, 0.02 M NaCl (pH 7.5), and 0.1% Tween 20), and incubated overnight with TBST containing 5% skim milk powder.…”

Section: Western Blotmentioning

confidence: 99%

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

Manoj

Griebel²,

Babiuk³

et al. 2003

The Journal of Immunology

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CD40-CD154 interactions play an important role in regulating humoral and cell-mediated immune responses. Recently, these interactions have been exploited for the development of therapeutic and preventive treatments. The objective of this study was to test the ability of bovine CD154 to target a plasmid-encoded Ag to CD40-expressing APCs. To achieve this, a plasmid coding for bovine CD154 fused to a truncated secreted form of bovine herpesvirus 1 glycoprotein D (tgD), pSLIAtgD-CD154, was constructed. The chimeric tgD-CD154 was expressed in vitro in COS-7 cells and reacted with both glycoprotein D- and CD154-specific Abs. Both tgD and tgD-CD154 were capable of binding to epithelial cells, whereas only tgD-CD154 bound to B cells. Furthermore, dual-labeling of ovine PBMCs revealed that tgD-CD154 was bound by primarily B cells. The functional integrity of the tgD-CD154 chimera was confirmed by the induction of both IL-4-dependent B cell proliferation and tgD-specific lymphoproliferative responses in vitro. Finally, sheep immunized with pSLIAtgD-CD154 developed a more rapid primary tgD-specific Ab response and a significantly stronger tgD-specific secondary response when compared with animals immunized with pSLIAtgD and control animals. Similarly, virus-neutralizing Ab titers were significantly higher after secondary immunization with pSLIAtgD-CD154. These results demonstrate that using CD154 to target plasmid-expressed Ag can significantly enhance immune responses induced by a DNA vaccine.

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“…Bajo estas circunstancias, una prueba diagnóstica alternativa tiene que ser comparada con un test estándar. La concordancia o conformidad entre ambas pruebas se expresa por el valor kappa (18). En los resultados del presente trabajo, usando los sueros en dilución 1:50, el valor kappa fue de 0.94 con un nivel de confianza del 95%.…”

Section: Resultsunclassified

“…Por lo tanto, en la práctica los clínicos tienen que utilizar con frecuencia reacciones en las que no hay estimación cuantitativa de sensibilidad y especificidad; admitiendo tácitamente que el valor predictivo puede ser suficientemente aceptable con fines prác-ticos (18).…”

Section: Discussionunclassified

Evaluación de la prueba ELISA usando una cepa de campo como antígeno, para detectar anticuerpos contra el virus de la Rinotraqueitis Bovina Infecciosa

Navarrete

Vera

Ramírez

et al. 2004

nova

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ResumenEl siguiente estudio estableció un inmunoensayo enzimático ELISA, usando partículas virales fraccionadas de una cepa de campo del virus IBR, este mostró una alta sensibilidad y especificidad y una baja tasa de falsos negativos y positivos. Los sueros bovinos positivos y negativos, con los cuales se normalizó la técnica de ELISA, fueron analizados por seroneutralización para determinar anticuerpos anti IBR; se recolectaron sueros negativos y positivos con títulos 1:4 a 1:128 y se consideró la dilución del suero bovino 1:50, como la dilución óptima para la técnica de ELISA. Por otra parte, se utilizó el coeficiente de variación BR, para analizar el grado de repetitibilidad de las absorbancias obtenidas de los sueros positivos y negativos, se obtuvo resultados menores del 20%, lo que indico una adecuada repetitibilidad y un bajo coeficiente de variación. La sensibilidad y especificidad encontrada fue de 96 y 98% respectivamente, teniendo como prueba de referencia la seroneutralización. Los resultados obtenidos, determinan que la prueba de ELISA con partículas virales fraccionadas de una cepa de campo, detecta anticuerpos anti IBR con alta sensibilidad y especificidad, siendo una herramienta para el mejoramiento diagnóstico de la enfermedad.Palabras Claves: ELISA, seroneutralización, rinotraqueitis bovina infecciosa, anticuerpos, mejoramiento diagnóstico. Abstract Evaluation of ELISA test use of field strain (antigen) to detect antibodies against InfectiousBovine Rhinotracheitis virus.The following study set up an enzymatic immune test (ELISA) using viral particles broke up of field strain of IBR virus; it showed a high sensibility and specificity and low rate of false negatives and positives. Positive and negative bovine serum which ELISA technique was normalized, were analyzed by seroneutralization to determine anti IBR antibodies; It was collected positive and negative serum with titles 1:4 to 1:128 and was considered dilution of bovine serum 1:50 as optimal dilution to ELISA technique.On the other hand, coefficient of variation BR was used to analyze the rehearsing of absorbance obtained by positive and negative serum. The results obtained were less than 20%, which indicated an appropriate

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Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: Characterization of their biochemical and immunological properties

Cited by 135 publications

References 28 publications

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

Evaluación de la prueba ELISA usando una cepa de campo como antígeno, para detectar anticuerpos contra el virus de la Rinotraqueitis Bovina Infecciosa

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