Interactions of mussel-inspired polymeric nanoparticles with gastric mucin: Implications for gastro-retentive drug delivery

Sunoqrot, Suhair; Hasan, Lina; Alsadi, Aya; Hamed, Rania; Tarawneh, Ola

doi:10.1016/j.colsurfb.2017.05.005

Cited by 38 publications

(17 citation statements)

References 45 publications

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“…In this work, commercially available mucin solutions have been prepared at concentrations between 0.15 and 5 mg/mL, as at this concentration, mucin is present as fully dissolved polymer (free mucin molecules). Moreover, similar concentration ranges have been previously reported in the literature on mucin interactions in binary systems [26,39].…”

Section: Discussionsupporting

confidence: 86%

“…Sunoqrot and colleagues reported that methoxy polyethylene glycol-b-poly(ε-caprolactone) nanoparticles (NPs) showed interaction with mucin molecules at any mucin:NP ratio (in w/w), from 0.25 (w/w) to 4.0 (w/w) via Schiff base and Michael addition reactions instead of electrostatic interactions, as the ζ potential of the mucin and NPs in the working conditions was close to neutrality. In such conditions, only bigger aggregates were formed at a mucin:NP ratio higher than 1.0 (w/w) [39]. Boya and colleagues also reported that the hydrodynamic size of metal NP coated with pluronic 127 increased 3 to 4 times after the interaction with mucin.…”

Section: Discussionmentioning

confidence: 98%

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Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin

et al. 2020

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Mucins are glycoproteins present in all mucosal surfaces and in secretions such as saliva. Mucins are involved in the mucoadhesion of nanodevices carrying bioactive molecules to their target sites in vivo. Oil-in-water nanocapsules (NCs) have been synthesised for carrying N,N′-(di-m-methylphenyl)urea (DMTU), a quorum-sensing inhibitor, to the oral cavity. DMTU-loaded NCs constitute an alternative for the treatment of plaque (bacterial biofilm). In this work, the stability of the NCs after their interaction with mucin is analysed. Mucin type III from Sigma-Aldrich has been used as the mucin model. Mucin and NCs were characterised by the multi-detection asymmetrical flow field-flow fractionation technique (AF4). Dynamic light scattering (DLS) and ζ-potential analyses were carried out to characterise the interaction between mucin and NCs. According to the results, loading DMTU changes the conformation of the NC. It was also found that the synergistic interaction between mucin and NCs was favoured within a specific range of the mucin:NC ratio within the first 24 h. Studies on the release of DMTU in vitro and the microbial activity of such NCs are ongoing in our lab.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin

et al. 2020

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“…mPEG-PCL copolymers were synthesized by ring-opening polymerization of CL as previously described [ 24 , 26 ], where mPEG served as the macroinitiator and stannous octoate as the catalyst to form mPEG-PCL block copolymers. PCL chain length was varied by controlling the mPEG:CL feed ratio ( Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

“…mPEG-PCL can be synthesized with varying chain lengths of the biodegradable block (i.e., PCL) in order to modulate its physicochemical properties. It can also be used to prepare NPs with different architectures such as matrix-type nanospheres (NS) [ 24 ], reservoir-type (core/shell) nanocapsules (NC) [ 25 ], as well as polymeric micelles [ 26 ]. In this study, in order to reach an optimized NP formulation for TQ, mPEG-PCL NPs were fabricated using different PCL chain lengths in the form of matrix-type NS or reservoir-type NC.…”

Section: Introductionmentioning

confidence: 99%

Development of a Thymoquinone Polymeric Anticancer Nanomedicine through Optimization of Polymer Molecular Weight and Nanoparticle Architecture

et al. 2020

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Thymoquinone (TQ) is a water-insoluble natural compound isolated from Nigella sativa that has demonstrated promising chemotherapeutic activity. The purpose of this study was to develop a polymeric nanoscale formulation for TQ to circumvent its delivery challenges. TQ-encapsulated nanoparticles (NPs) were fabricated using methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-PCL) copolymers by the nanoprecipitation technique. Formulation variables included PCL chain length and NP architecture (matrix-type nanospheres or reservoir-type nanocapsules). The formulations were characterized in terms of their particle size, polydispersity index (PDI), drug loading efficiency, and drug release. An optimized TQ NP formulation in the form of oil-filled nanocapsules (F2-NC) was obtained with a mean hydrodynamic diameter of 117 nm, PDI of 0.16, about 60% loading efficiency, and sustained in vitro drug release. The formulation was then tested in cultured human cancer cell lines to verify its antiproliferative efficacy as a potential anticancer nanomedicine. A pilot pharmacokinetic study was also carried out in healthy mice to evaluate the oral bioavailability of the optimized formulation, which revealed a significant increase in the maximum plasma concentration (Cmax) and a 1.3-fold increase in bioavailability compared to free TQ. Our findings demonstrate that the versatility of polymeric NPs can be effectively applied to design a nanoscale delivery platform for TQ that can overcome its biopharmaceutical limitations.

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“…4d), which confirmed that bare PLGA and PLGA-PVA NPs are more likely to bonds with mucin. Likewise, turbidity measurements and mucin absorption percentage were also performed to investigate the interaction between NPs and mucin [41][42][43] ( Supplementary Fig. 5a and 5b), which altogether pointed to the fact that PLGA-PEG and PLGA-PDA NPs interacted much less with mucin than those of bare PLGA and PLGA-PVA NPs.…”

Section: Exploration Of the Interaction Mechanisms Between Pva-dopa Fmentioning

confidence: 98%

A mussel-inspired film for adhesion of wet buccal tissue and efficient buccal drug delivery

Pei

Duan

et al. 2020

Preprint

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Administration of drug via the buccal route has attracted much attention in recent years. However, developing system with satisfactory adhesion in wet conditions and drug bioavailability still remains a challenge. Here, we propose a mussel inspired mucoadhesive film. Ex vivo porcine and in vivo rat models show that the film can achieve strong adhesion with wet buccal tissues. We also demonstrate that the film exhibits tunable mucoadhesion strength and erosion rate. The adhesion mechanism of this film relies on both physical association and covalent bonding between the film and mucus. Then, polydopamine (PDA) modified nanoparticles (NPs) are incorporated into the film and the PDA NPs loaded films show superior advantages to transport across multiple barriers of buccal mucosa with improved drug bioavailability and therapeutic efficacy in oral mucositis models. We anticipate that this platform might aid the development of tissue adhesives and inspire the design of nanoparticle-based buccal delivery systems.

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Interactions of mussel-inspired polymeric nanoparticles with gastric mucin: Implications for gastro-retentive drug delivery

Cited by 38 publications

References 45 publications

Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin

Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin

Development of a Thymoquinone Polymeric Anticancer Nanomedicine through Optimization of Polymer Molecular Weight and Nanoparticle Architecture

A mussel-inspired film for adhesion of wet buccal tissue and efficient buccal drug delivery

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