Interactions of phospholipid- and poly(ethylene glycol)-modified surfaces with biological systems: relation to physico-chemical properties and mechanisms

Vermette, Patrick; Meagher, Laurence

doi:10.1016/s0927-7765(02)00160-1

Cited by 218 publications

(259 citation statements)

References 257 publications

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“…In the current study, the PEG molecules could extend enoxacin away from the Ti surface, allowing enoxacin to enter the bacterial cell wall and bind to the bacterium's DNA gyrase and topoisomerase IV. In addition, after implantation plasma proteins are recruited to the implant surface and form a plasma layer, which can disable the antibacterial coating surface (36); however, PEG has the ability to inhibit protein binding and avoids the adverse effects described above (37)(38)(39), and this function has also been proved by the current study. Our previous study demonstrated that the covalent cross-linking under the action of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) enhanced the stability of the coating, in contrast to that of the controlled-release system (40).…”

Section: Discussionmentioning

confidence: 73%

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Nie

Long

et al. 2017

Antimicrob Agents Chemother

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Infection is one of the most important causes of titanium implant failure in vivo. A developing prophylactic method involves the immobilization of antibiotics, especially vancomycin, onto the surface of the titanium implant. However, these methods have a limited effect in curbing multiple bacterial infections due to antibiotic specificity. In the current study, enoxacin was covalently bound to an aminefunctionalized Ti surface by use of a polyethylene glycol (PEG) spacer, and the bactericidal effectiveness was investigated in vitro and in vivo. The titanium surface was amine functionalized with 3-aminopropyltriethoxysilane (APTES), through which PEG spacer molecules were covalently immobilized onto the titanium, and then the enoxacin was covalently bound to the PEG, which was confirmed by X-ray photoelectron spectrometry (XPS). A spread plate assay, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to characterize the antimicrobial activity. For the in vivo study, Ti implants were inoculated with methicillin-resistant Staphylococcus aureus (MRSA) and implanted into the femoral medullary cavity of rats. The degree of infection was assessed by radiography, micro-computed tomography, and determination of the counts of adherent bacteria 3 weeks after surgery. Our data demonstrate that the enoxacin-modified PEGylated Ti surface effectively prevented bacterial colonization without compromising cell viability, adhesion, or proliferation in vitro. Furthermore, it prevented MRSA infection of the Ti implants in vivo. Taken together, our results demonstrate that the use of enoxacin-modified Ti is a potential approach to the alleviation of infections of Ti implants by multiple bacterial species.

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Section: Discussionmentioning

confidence: 73%

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Nie

Long

et al. 2017

Antimicrob Agents Chemother

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“…[22][23][24][25][26][27] The covalent grafting of PEG onto a variety of substrates, including silicon, 24,28 polyurethane-urea, 29 glass, 30,31 fluorinated ethylene-propylene copolymers, 32 and polysulfone membranes, 33 has been reported along with a quite satisfactory protein-repellent effect. It may be anticipated that the proteinrepellent properties of PEG would prevent an extracellular matrix from being formed and, thus, cells from adhering.…”

Section: Introductionmentioning

confidence: 99%

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

et al. 2007

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Cataract surgery is a routine ophthalmologic intervention resulting in replacement of the opacified natural lens by a polymeric intraocular lens (IOL). A main postoperative complication, as a result of protein adsorption and lens epithelial cell (LEC) adhesion, growth, and proliferation, is the secondary cataract, referred to as posterior capsular opacification (PCO). To avoid PCO formation, a poly(ethylene glycol) (PEG) chemical coating was created on the surface of hydrogel IOLs. Attenuated total reflectance Fourier transform infrared spectroscopy, “captive bubble” and “water droplet” contact angle measurements, and atomic force microscopy analyses proved the covalent grafting of the PEG chains on the IOL surface while keeping unchanged the optical properties of the initial material. A strong decrease of protein adsorption and cell adhesion depending on the molar mass of the grafted PEG (1100, 2000, and 5000 g/mol) was observed by performing the relevant in vitro tests with green fluorescent protein and LECs, respectively. Thus, the study provides a facile method for developing materials with nonfouling properties, particularly IOLs.

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“…For this purpose, the modification of biointerfaces a͒ Author to whom correspondence should be addressed; electronic mail: brashjl@mcmaster.ca with poly͑ethylene oxide͒ ͑PEO͒ ͑Refs. 6 and 7͒ or with polymers based on phosphorylcholine [8][9][10] ͑PC͒ has been found to be effective.…”

Section: Introductionmentioning

confidence: 99%

Characterization of protein resistant, grafted methacrylate polymer layers bearing oligo(ethylene glycol) and phosphorylcholine side chains by neutron reflectometry

Feng

Nieh²,

Zhu

et al. 2007

Biointerphases

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Neutron reflectometry was used to investigate the structures of end-tethered protein resistant polymer layers based on poly(oligo(ethylene glycol) methyl ether methacrylate) [poly(OEGMA)] and poly(2-methacryloyloxyethyl phosphorylcholine) [poly(MPC)]. Layers having different graft densities were studied in both the dry and wet states. A stretched parabolic model was used to fit the neutron data, resulting in a one-dimensional scattering length density profile of the polymer volume fraction normal to the film. Measured in D2O, the cutoff thicknesses of OEGMA and MPC layers at high graft density (0.39 chains/nm2 for OEGMA and 0.30 chains/nm2 for MPC) and a chain length of 200 repeat units were 450 and 470 Å, respectively, close to their contour length of 500 Å, suggesting that the grafts become highly hydrated when exposed to water. It was also found that at similar graft density and chain length, the volume fraction profiles of poly(OEGMA) and poly(MPC) layers are similar, in line with the authors’ previous results showing that these surfaces have similar protein resistance [W. Feng et al., BioInterphases 1, 50 (2006)]. The possible correlation of protein resistance to water content as indicated by the average number of water molecules per ethylene oxide (Nw,EO) or phosphorylcholine (Nw,PC) moiety was investigated. Nw,EO and Nw,PC, estimated from the volume fraction data, increased with decreasing graft density, and when compared to the reported number of water molecules in the hydration layers of EO and PC residues, led to the conclusion that water content slightly greater than the water of hydration resulted in protein resistant surfaces, whereas water content either less than or greatly in excess of the water of hydration resulted in layers of reduced protein resistance.

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Interactions of phospholipid- and poly(ethylene glycol)-modified surfaces with biological systems: relation to physico-chemical properties and mechanisms

Cited by 218 publications

References 257 publications

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

Characterization of protein resistant, grafted methacrylate polymer layers bearing oligo(ethylene glycol) and phosphorylcholine side chains by neutron reflectometry

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