Interactions of Pluronics with phospholipid monolayers at the air–water interface

Novel mucus-penetrating liposomes as a potential oral drug delivery system: preparation, in vitro characterization, and enhanced cellular uptake

“…19 It is widely accepted that Pluronic polymers can stabilize and seal damaged lipid bilayer membranes. [34][35][36] Considering that the PF127 is adsorbed on the outer leaflet of the bilayer (PF127-adsorbed liposomes), it may be easily lost by simple diffusion. It might then interact with the cell membrane, altering the membrane fluidity and membrane interaction, and finally stabilizing the membrane structure.…”

Interfacial properties of Pluronics and the interactions between Pluronics and cholesterol/DPPC mixed monolayers

“…Pluronic F68 was demonstrated to serve as a membrane sealant which only interacts with compromised bilayers. After the membrane integrity is restored, Pluronic F68 would be "squeezed out" of the membrane [8][9][10][11][12]. The "squeezed out" phenomenon was previously mentioned by Weingarten et al [13], and this characteristic was generally perceived to be suitable for medical purpose [12].…”

“…In the present study, phospholipid monolayer system at the air-water interface was utilized to represent the outer leaflet of the membrane as demonstrated before [11,23,24]. In order to elucidate the structural effects of Pluronics, Pluronics L61, P65, F68, P84, P123, L35, and P105 were selected so as to gain insight into the influence of PEO chain, PPO chain, and molecular weight, respectively.…”

Interaction of poloxamine block copolymers with lipid membranes: Role of copolymer structure and membrane cholesterol content

“…It should be noticed that at low values (2.5-10 mN/m), both PEO and PPO blocks are close to the interface, which means that interactions between PEO and the polar region of DPPC, and between PPO and the apolar region of DPPC are feasible. In the case of Pluronics, it has been previously reported that intercalation of the copolymer in the lipid film causes its disordering, which leads to an increase in the surface area occupied by the molecules [15]. This was observed for Pluronic P85 (Fig.…”

“…In addition to healthy cell membrane. From a practical point of view, copolymer insertion can be useful for selective sealing of porated or permeabilized regions of cell membranes [13][14][15]. Once the membrane recovers the lipid ordering, the copolymer unimers are expected to be squeezed out.…”