Interactions of Polycomb and trithorax with cis regulatory regions of Ultrabithorax during the development of Drosophila melanogaster.

Castelli-Gair, James; Garcı́a-Bellido, Antonio

doi:10.1002/j.1460-2075.1990.tb07875.x

Cited by 40 publications

(22 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5, B and C). This repression-at-a-distance is similar to that observed with M33 (41) and is consistent with other experiments showing that several mammalian and Drosophila PcG proteins can exert their repressive effects over several kilobases (4,59,67).…”

Section: Hpc3 Is a Human Orthologue Of Drosophila Polycomb-supporting

confidence: 91%

HPC3 Is a New Human Polycomb Orthologue That Interacts and Associates with RING1 and Bmi1 and Has Transcriptional Repression Properties

Bárdos

Saurin

Tissot

et al. 2000

Journal of Biological Chemistry

View full text Add to dashboard Cite

Polycomb group (PcG) proteins were first described in Drosophila as factors responsible for maintaining the transcriptionally repressed state of Hox/homeotic genes in a stable and heritable manner throughout development. A growing number of vertebrate genes related to the Drosophila PcG proteins have recently been identified, including two Polycomb orthologues, Pc2 and M33. PcG proteins form multiprotein complexes, termed PcG bodies, that are thought to repress transcription by altering chromatin structure. Here we report the identification and characterization of HPC3 (human Polycomb 3), a novel PcG protein isolated in a yeast two-hybrid screen using human RING1 as bait. HPC3 shows strong sequence similarity to Drosophila Pc and also to vertebrate Pc2 and M33, particularly within the chromodomain and C-box. Previous studies indicate that M33 and human Pc2 (HPC2) can interact with RING1, and we show here that HPC3 also binds to RING1. This interaction is dependent upon the HPC3 C-box but, only partially on the RING finger of RING1. In contrast to HPC2, HPC3 interactions with RING1 are only observed in vivo with covalently modified forms of RING1. HPC3 also colocalizes with other PcG proteins in human PcG bodies. Consistent with its role as a PcG member, HPC3 is able to act as a long range transcriptional silencer when targeted to a reporter gene by a heterologous DNA-binding domain. Taken together, these data suggest that HPC3 is part of a large multiprotein complex that also contains other PcG proteins and is involved in repression of transcriptional activity.

show abstract

Section: Hpc3 Is a Human Orthologue Of Drosophila Polycomb-supporting

confidence: 91%

HPC3 Is a New Human Polycomb Orthologue That Interacts and Associates with RING1 and Bmi1 and Has Transcriptional Repression Properties

Bárdos

Saurin

Tissot

et al. 2000

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…This VOL. 22,2002 Polycomb BINDING TO GRH TARGET GENES 1941 on May 9, 2018 by guest http://mcb.asm.org/ regulation is mediated in part by PcG response elements (PREs), cis-acting DNA elements which maintain the expression boundaries of homeotic genes (12,14,24,43,60). More recently, PcG binding has also been demonstrated outside PREs, in core promoters (44).…”

Section: Discussionmentioning

confidence: 99%

Binding of the RING Polycomb Proteins to Specific Target Genes in Complex with the grainyhead-Like Family of Developmental Transcription Factors

Tuckfield

Clouston

Wilanowski

et al. 2002

Molecular and Cellular Biology

View full text Add to dashboard Cite

The Polycomb group (PcG) of proteins represses homeotic gene expression through the assembly of multiprotein complexes on key regulatory elements. The mechanisms mediating complex assembly have remained enigmatic since most PcG proteins fail to bind DNA. We now demonstrate that the human PcG protein dinG interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription. The CP2-dinG interaction is conserved in evolution with the Drosophila factor grainyhead binding to dring, the fly homologue of dinG. Electrophoretic mobility shift assays demonstrate that the grh-dring complex forms on regulatory elements of genes whose expression is repressed by grh but not on elements where grh plays an activator role. These observations reveal a novel mechanism by which PcG proteins may be anchored to specific regulatory elements in developmental genes.

show abstract

“…It appears that Pc-G proteins can exert their repressive effects over several kilobases in D. melanogaster (6,43). We therefore analyzed the effect of changing the distance between the LexA sites and the activator site on repression by the LexA fusions.…”

Section: Methodsmentioning

confidence: 99%

Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells.

1994

View full text Add to dashboard Cite

The Polycomb group (Pc-G) genes are essential for maintaining the proper spatially restricted expression pattern of the homeotic loci during Drosophila development. The Pc-G proteins appear to function at target loci to maintain a state of transcriptional repression. The murine oncogene bmi-1 has significant homology to the Pc-G gene Posterior sex combs (Psc) and a highly related gene, Suppressor two of zeste [Su(z)2]. We show here that the proteins encoded by bmi-1 and the Pc-G genes Polycomb (Pc) and Psc as well as Su(z)2 mediate repression in mammalian cells when targeted to a promoter by LexA in a cotransfection system. These fusion proteins repress activator function by as much as 30-fold, and the effect on different activation domains is distinct for each Pc-G protein. Repression is observed when the LexA fusion proteins are bound directly adjacent to activator binding sites and also when bound 1,700 bases from the promoter. These data demonstrate that the products of the Pc-G genes can significantly repress activator function on transiently introduced DNA. We suggest that this function contributes to the stable repression of targeted loci during development.

show abstract

Interactions of Polycomb and trithorax with cis regulatory regions of Ultrabithorax during the development of Drosophila melanogaster.

Cited by 40 publications

References 48 publications

HPC3 Is a New Human Polycomb Orthologue That Interacts and Associates with RING1 and Bmi1 and Has Transcriptional Repression Properties

HPC3 Is a New Human Polycomb Orthologue That Interacts and Associates with RING1 and Bmi1 and Has Transcriptional Repression Properties

Binding of the RING Polycomb Proteins to Specific Target Genes in Complex with the grainyhead-Like Family of Developmental Transcription Factors

Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells.

Contact Info

Product

Resources

About