Interactive effects of aging and aerobic capacity on energy metabolism–related metabolites of serum, skeletal muscle, and white adipose tissue

Abstract

Aerobic capacity is a strong predictor of longevity. With aging, aerobic capacity decreases concomitantly with changes in whole body metabolism leading to increased disease risk. To address the role of aerobic capacity, aging, and their interaction on metabolism, we utilized rat models selectively bred for low and high intrinsic aerobic capacity (LCRs/HCRs) and compared the metabolomics of serum, muscle, and white adipose tissue (WAT) at two time points: Young rats were sacrificed at 9 months of age, and old rats were sacrificed at 21 months of age. Targeted and semi-quantitative metabolomics analysis was performed on the ultra-pressure liquid chromatography tandem mass spectrometry (UPLC-MS) platform. The effects of aerobic capacity, aging, and their interaction were studied via regression analysis. Our results showed that high aerobic capacity is associated with an accumulation of isovalerylcarnitine in muscle and serum at rest, which is likely due to more efficient leucine catabolism in muscle. With aging, several amino acids were downregulated in muscle, indicating more efficient amino acid metabolism, whereas in WAT less efficient amino acid metabolism and decreased mitochondrial β-oxidation were observed. Our results further revealed that high aerobic capacity and aging interactively affect lipid metabolism in muscle and WAT, possibly combating unfavorable aging-related changes in whole body metabolism. Our results highlight the significant role of WAT metabolism for healthy aging.

The sex-specific metabolic signature of C57BL/6NRj mice during aging

“…6 H), were steadily decreasing in female and male skeletal muscle tissues during aging. Reduced levels of amino acids might be due to impaired amino acid uptake 58 or resulting from enhanced amino acid metabolism 59 with aging. The level of lactic acid (Fig.…”

Replicative ageing perturbs the metabolic signature of murine C2C12 skeletal myotubes

“…Overall, the metabolic signatures of control and aged myotubes were profoundly different, which was largely due to lower levels of aminoacyl-tRNA and BCAA biosynthesis-related metabolites in aged myotubes. Reductions in the abundance of branched chain amino acids in ageing rodent muscle have been reported previously (Zhuang et al, 2021) and suggest a lowered potential for translation and protein synthesis, with potential implications for the fusion capacity of aged muscle cells (Brown et al, 2017). Furthermore, given that branched chain amino acids can be metabolised and can participate in the TCA cycle (Ye et al, 2020), these metabolites may have been sequestered to support metabolic flux in aged myotubes.…”

Clustering Disease of Clostridioides Difficile Infection: Implication for the Management in Internal Medicine

“…Elements not of a physiological nature such as economic difficulties, poor functional autonomy, social isolation, neurodegenerative diseases, and senile psychoses could affect the nutritional status of this segment of the population [136][137][138]. Constitutional factors affecting the composition of body mass, with an increase in fat mass at the expense of muscle and bone mass the decrease in plasma concentration and total body water content are added elements that can affect the sensitivity to infections, the immune response, and the response to antimicrobial therapy [139][140][141][142].…”