Interazione linfociti-macrofagi nella reazione di rigetto di tumori ascite

Bellelli, L; Nista, Anna; Russo, Matteo Antonio; Sezzi, Maria Luisa

doi:10.1177/030089167205800415

Cited by 3 publications

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“…However, this was not observed. Thus we must consider that in mice specifically immunized against L I210 leukemia and Ehrlich's adenocarcinoma the rejection reaction starts immediately, is well established 24-48 hours after the challenge, and in the following 24 hours the tumor cells are all phagocytized by the peritoneal macrophages [1]. In experiments here described the drastic depletion of C'3 caused by the CoF injections was obtained for at least five days after the tumor challenge, certainly longer than the time required for the rejection reaction in control immunized mice.…”

Section: Discussionmentioning

confidence: 99%

“…Immunization of DBA/2 and Swiss mice against LI 210 leukemia and Ehrlich's adenocarcinoma respectively (both in the ascites form) was obtained as previously described [1,2], Once every seven days for four weeks, mice were injected intraperitoneally with 3 x 107 heavily irradiated tumor cells (14,000 r from a 250 kV generator 1,000 r/min; Al 1 mm). Lyophilized CoF (Cordis) was reconstituted with sterile distilled water to 100 anticomplementary units per ml.…”

Section: Methodsmentioning

confidence: 99%

“…In previous papers we described the immune reaction that induces the rejection of some experimental tumors in specifically immunized mice [1,2], We observed that this rejection is the result of the active participation of lymphocytes and macrophages and that phagocytosis by macrophages of cellular debris and even of whole tumor cells (apparently intact and unmodified) is frequently found. There was evidence that IgM may take part in the reaction: in experiments of im munofluorescence with IgM antisera the cell membranes of lymphocytes, macrophages and tumor cells were all fluor escent.…”

Section: Introductionmentioning

confidence: 99%

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C’3 Participation in the Rejection of Some Experimental Tumors

Bellelli¹,

Sezzi²

1976

Oncology

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The possibility that C’3 participates in tumor rejection was investigated in DBA/2 mice previously immunized against L1210 leukemia and in Swiss mice previously immunized against Ehrlich’s adenocarcinoma. In both the cases, animals treated with an appropriate dose of cobra venom factor to produce a C’3 depletion for some days after the tumor challenge, developed the neoplasia and had a mortality rate analogous to that of non-immunized animals. Studies on the peritoneal washing cells obtained at different times after the challenge revealed that in C’3 depleted immunized mice IgM are present on lymphocytes, macrophages and tumor cells, as in the immunized controls, but no contact between the cells and no macrophage phagocytosis were observed and the number of tumor cells increased progressively. These findings indicate that C’3 is critically involved in the rejection of the experimental tumors considered.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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C’3 Participation in the Rejection of Some Experimental Tumors

Bellelli¹,

Sezzi²

1976

Oncology

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Lymphocyte-macrophage Interaction in the Rejection Reaction Against Isologous and Homologous Ascites Tumors.

Bellelli

Nista

Russo³

et al. 1974

Tumori

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The immunological rejection of two strains of Ehrlich adenocarcinoma ascites cells and of leukemia L1210 ascites cells was studied respectively in Swiss and DBA/2 mice previously immunized with irradiated tumor cells. During the first phases of the reaction cell contacts between lymphocytes and cancer cells, lymphocytes and macrophages and also between two lymphocytes were frequently observed. Afterwards macrophages surrounding tumor cells and macrophages loaded either with whole tumor cells or with cell debris were a usual finding. During the immunological rejection 7S globulin could not be detected on cancer cells by fluoresceine isothiocyanate labelled rabbit γ-globulin anti-7S mouse globulin. Cytotoxicity test performed on 51Cr-labelled tumor cells showed that peritoneal cells are more active than spleen and lymph node cells. Some hypotheses on the observed phenomena are discussed.

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IgM Participation in Tumor Rejection by Immunized MICE

Bellelli

Nista

Sezzi

1977

Tumori

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IgG and IgM participation in tumor rejection was studied in DBA/2 mice immunized against L1210 leukemia and in Swiss mice immunized against Ehrlich adenocarcinoma. In both the systems, IgM globulins seem to be implicated and are present on the cell surface of macrophages, lymphocytes and cancer cells, while IgG globulins are present only on some lymphocytes. IgM are also present in the peritoneal washing fluids (obtained 24 h of the control group. In the former group, protein content is about three times higher than that in the control group, while the relative amount of heavy proteins (18 S) and light proteins (7 S) is quite similar. These observations are discussed, as is the possibility that some complement components as C3 may participate in the reaction.

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Interazione linfociti-macrofagi nella reazione di rigetto di tumori ascite

Cited by 3 publications

References 0 publications

C’3 Participation in the Rejection of Some Experimental Tumors

C’3 Participation in the Rejection of Some Experimental Tumors

Lymphocyte-macrophage Interaction in the Rejection Reaction Against Isologous and Homologous Ascites Tumors.

IgM Participation in Tumor Rejection by Immunized MICE

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